LOCUS Exported 5332 bp ds-DNA circular SYN 01-DEC-2023 DEFINITION Express TagBFP labeled human Rab7L1.. ACCESSION . VERSION . KEYWORDS pTag-BFP-C-h-Rab7L1-c-Myc SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5332) AUTHORS Boothe T, Lim GE, Cen H, Skovso S, Piske M, Li SN, Nabi IR, Gilon P, Johnson JD TITLE Inter-domain tagging implicates caveolin-1 in insulin receptor trafficking and Erk signaling bias in pancreatic beta-cells. JOURNAL Mol Metab. 2016 Feb 10;5(5):366-78. doi: 10.1016/j.molmet.2016.01.009. eCollection 2016 May. PUBMED 27110488 REFERENCE 2 (bases 1 to 5332) AUTHORS . TITLE Direct Submission JOURNAL Exported Dec 1, 2023 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5332 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 61..364 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 365..568 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 519..539 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" CDS 608..1306 /codon_start=1 /product="monomeric blue fluorescent protein" /label=TagBFP /note="mammalian codon-optimized" /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC DLPSKLGHKLN" CDS 1958..1987 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" polyA_signal 2120..2241 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2157..2176) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 2211..2230 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(2248..2703) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2385..2406) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2597..2616 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2730..2834 /gene="bla" /label=AmpR promoter promoter 2836..3193 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2859..2879) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 3044..3179 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3106..3125 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3228..4022 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3282..3301) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3892..3911 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(4210..4229) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 4254..4301 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4630..5218 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5119..5138 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagaatc cgctagcggt 601 cgccaccatg agcgagctga ttaaggagaa catgcacatg aagctgtaca tggagggcac 661 cgtggacaac catcacttca agtgcacatc cgagggcgaa ggcaagccct acgagggcac 721 ccagaccatg agaatcaagg tggtcgaggg cggccctctc cccttcgcct tcgacatcct 781 ggctactagc ttcctctacg gcagcaagac cttcatcaac cacacccagg gcatccccga 841 cttcttcaag cagtccttcc ctgagggctt cacatgggag agagtcacca catacgaaga 901 cgggggcgtg ctgaccgcta cccaggacac cagcctccag gacggctgcc tcatctacaa 961 cgtcaagatc agaggggtga acttcacatc caacggccct gtgatgcaga agaaaacact 1021 cggctgggag gccttcaccg agacgctgta ccccgctgac ggcggcctgg aaggcagaaa 1081 cgacatggcc ctgaagctcg tgggcgggag ccatctgatc gcaaacatca agaccacata 1141 tagatccaag aaacccgcta agaacctcaa gatgcctggc gtctactatg tggactacag 1201 actggaaaga atcaaggagg ccaacaacga gacctacgtc gagcagcacg aggtggcagt 1261 ggccagatac tgcgacctcc ctagcaaact ggggcacaag cttaattccg gactcagatc 1321 tcgagctcaa atgggcagcc gcgaccacct gttcaaagtg ctggtggtgg gggacgccgc 1381 agtgggcaag acgtcgctgg tgcagcgata ttcccaggac agcttcagca aacactacaa 1441 gtccacggtg ggagtggatt ttgctctgaa ggttctccag tggtctgact acgagatagt 1501 gcggcttcag ctgtgggata ttgcagggca ggagcgcttc acctctatga cacgattgta 1561 ttatcgggat gcctctgcct gtgttattat gtttgacgtt accaatgcca ctaccttcag 1621 caacagccag aggtggaaac aggacctaga cagcaagctc acactaccca atggagagcc 1681 ggtgccctgc ctgctcttgg ccaacaagtg tgatctgtcc ccttgggcag tgagccggga 1741 ccagattgac cggttcagta aagagaacgg tttcacaggt tggacagaaa catcagtcaa 1801 ggagaacaaa aatattaatg aggctatgag agtcctcatt gaaaagatga tgagaaattc 1861 cacagaagat atcatgtctt tgtccaccca aggggactac atcaatctac aaaccaagtc 1921 ctccagctgg tcctgctgca cgcgtacgcg gccgctcgag cagaaactca tctcagaaga 1981 ggatctgtaa ggatccaccg gatctagata actgatcata atcagccata ccacatttgt 2041 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat 2101 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa 2161 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 2221 caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg ttaaaattcg 2281 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc 2341 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga 2401 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg 2461 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag 2521 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga 2581 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg 2641 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg 2701 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa 2761 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt 2821 gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg 2881 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc 2941 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca 3001 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc 3061 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc 3121 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct 3181 aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag 3241 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg 3301 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc 3361 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag 3421 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca 3481 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat 3541 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata 3601 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac 3661 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc 3721 tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg 3781 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg 3841 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta 3901 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg 3961 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct 4021 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga 4081 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc 4141 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg 4201 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat 4261 aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc 4321 ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt 4381 ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa 4441 cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt 4501 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac 4561 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 4621 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 4681 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 4741 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg 4801 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 4861 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 4921 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 4981 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 5041 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 5101 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 5161 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 5221 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt 5281 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc at //