LOCUS Exported 993 bp ds-DNA linear SYN 23-MAY-2021 DEFINITION mammalian expression of Zdk1. ACCESSION . VERSION . KEYWORDS pTriEx-mCherry-Zdk1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 993) AUTHORS Wang H, Vilela M, Winkler A, Tarnawski M, Schlichting I, Yumerefendi H, Kuhlman B, Liu R, Danuser G, Hahn KM TITLE LOVTRAP: an optogenetic system for photoinduced protein dissociation. JOURNAL Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926. PUBMED 27427858 REFERENCE 2 (bases 1 to 993) AUTHORS . TITLE Direct Submission JOURNAL Exported May 23, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..993 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 1..708 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(146..164) /label=mCherry-R /note="mCherry, reverse primer" CDS 721..897 /codon_start=1 /product="""Zdark"" protein, derived from the Z subunit of protein A, that binds to the dark state of LOV2 and is released upon irradiation (Wang et al., 2016)" /label=Zdk1 /translation="MVDNKFNKEKTRAGAEIHSLPNLNVEQKFAFIVSLFDDPSQSANL LAEAKKLNDAQAPK" CDS 916..933 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 940..960 /codon_start=1 /product="tobacco etch virus (TEV) protease recognition and cleavage site" /label=TEV site /translation="ENLYFQG" CDS 967..990 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" ORIGIN 1 atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag 61 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc 121 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc 181 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac 241 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc 301 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac 361 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccctgta 421 atgcagaaga agactatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc 481 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct 541 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc 601 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa 661 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaaggg ttctggatcc 721 atggtggata acaaattcaa taaagaaaag acgcgtgccg gtgcggaaat ccattctctg 781 ccaaacctga atgttgagca gaagtttgcc ttcatcgtga gcctgtttga tgatccatct 841 cagagcgcca atctgctggc cgaagccaaa aaactgaacg atgcccaggc cccaaaaggt 901 ggttctggtg gtagccatca tcatcatcat catggcggtg aaaatctgta tttccagggc 961 ggccgcgact acaaggacga cgacgacaaa taa //