LOCUS Exported 796 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION 3rd generation lentiviral gRNA plasmid targeting EGFP. ACCESSION . VERSION . KEYWORDS EGFP gRNA (BRDN0000563266) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 796) AUTHORS Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, Root DE TITLE Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. JOURNAL Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437. PUBMED 26780180 REFERENCE 2 (bases 1 to 796) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..796 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 109..128 /label=LKO.1 5' /note="Human U6 promoter, forward primer" misc_RNA 208..283 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" misc_feature 339..456 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" ORIGIN 1 attagaatta atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag 61 taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg 121 cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga 181 aacaccggag ctggacggcg acgtaaagtt ttagagctag aaatagcaag ttaaaataag 241 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgctttttta agcttggcgt 301 aactagatct tgagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt 361 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa 421 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga 481 gatccacttt ggcgccggct cgagggggcc cgggtgcaaa gatggataaa gttttaaaca 541 gagaggaatc tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc 601 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg 661 gtcggcaatt gatccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc 721 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc 781 gccgtgaacg ttcttt //