LOCUS       Exported               14386 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  expresses mouse Piezo1 with GFP.
ACCESSION   .
VERSION     .
KEYWORDS    mPiezo1-IRES-eGFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14386)
  AUTHORS   Coste B, Mathur J, Schmidt M, Earley TJ, Ranade S, Petrus MJ, Dubin 
            AE, Patapoutian A
  TITLE     Piezo1 and Piezo2 are essential components of distinct mechanically 
            activated cation channels.
  JOURNAL   Science. 2010 Oct 1;330(6000):55-60. doi: 10.1126/science.1193270. 
            Epub 2010 Sep 2.
  PUBMED    20813920
REFERENCE   2  (bases 1 to 14386)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14386
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      934..943
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     misc_feature    8639..9225
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8806..8823)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9033..9052
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             9226..9945
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             9226..9942
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9271..9292)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9532..9551)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9879..9900
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(9980..9997)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9986..10210
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10256..10684
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10343..10362)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10553..10574
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10693..10713)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10698..11027
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10878..11013
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10940..10959
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             11094..11888
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(11148..11167)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11758..11777
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    12062..12183
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12099..12118)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12153..12172
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12232..12248)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12232..12248)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12245..12267)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12256..12272
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(12280..12310)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12325..12346
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12463..12480)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12634..13219)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12714..12733)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(13390..14250)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14013..14032
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14251..14355)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaacgg gccctctaga ctcgagcggc cgcgccacca tggagccgca cgtgctgggc
      961 gccgggctct actggctgtt gctgccctgc acgctcctgg cggcctccct gttacgcttc
     1021 aatgctctct cgctggtcta tttgctgttt ctactgctgc tgccctggct tccaggcccc
     1081 tcaagacaca gcataccagg tcacacaggt cgcctgctcc gtgcactact ctgcctcagc
     1141 ctcctcttcc tggtggccca ccttgccttt cagatatgcc tacacaccgt gcctcacctg
     1201 gaccagtttc tgggacaaaa cggtagcctt tgggtgaagg tgtctcaaca cataggggtt
     1261 acgaggctgg acctgaagga catctttaac accaccaggc tggtagcacc tgacctggga
     1321 gtgctgctgg cgtcctccct ttgccttggc ctctgtggac gcctcacgag gaaagccggg
     1381 cagagtcggc gcacccagga gctgcaggat gatgacgatg acgatgatga cgacgatgaa
     1441 gacatagatg ctgccccagc cgtggggctg aagggagccc ctgccctggc aaccaaacgc
     1501 aggctgtggc tggcctcccg cttccgggtc acggcccact ggctgctgat gacctctgga
     1561 cggacgctgg tcattgtgtt gctggccctg gcaggcatag cccacccttc ggccttctcc
     1621 agcatctacc tggtggtgtt cctggccatc tgcacctggt ggtcctgtca ctttcctctc
     1681 agccccctgg gctttaacac cctctgtgtc atggtgagct gttttggtgc cggccatctc
     1741 atttgcctat actgctatca gacaccattt atccaggaca tgttaccccc tgggaacatc
     1801 tgggccaggc tatttggtct caagaacttc gtagacctcc ctaactactc cagccccaac
     1861 gccctggtac tcaacactaa gcacgcctgg cccatctatg tgagtcccgg aatcctgctg
     1921 ctgctatatt acacagccac ctctctcctg aagctccaca agagctgtcc ctcagagctg
     1981 aggaaggaga cacccaggga ggatgaggag catgagctgg aactggacca cctggagcca
     2041 gagccccagg ctagggacgc cacccagggt gagatgccca tgaccacgga acctgacctt
     2101 gacaactgca ccgtgcatgt actaaccagc cagagccctg tccgccagcg tccagttcgc
     2161 cccaggctgg ctgagctgaa agagatgtca ccgctacatg gcctgggcca cctcatcatg
     2221 gaccagagct atgtgtgtgc cctgattgcc atgatggtgt ggagcatcat gtaccacagt
     2281 tggctgacct tcgtcctgct gctctgggcc tgcctcatct ggacggtgcg tagccgtcac
     2341 cagctggcta tgctctgctc gccctgcatc ctgctgtatg ggctgacgct ctgctgcctg
     2401 aggtatgtgt gggccatgga acttcctgag ctgcccacca ccctgggccc tgtcagcctg
     2461 caccagttgg gactggaaca cacacgctac ccttgcctgg acctcggtgc catgctcctc
     2521 tatctgctca cattctggct ccttctgcgt cagttcgtga aggagaagct gctgaagaag
     2581 caaaaggtgc ctgcggcgct gctggaggtc acggtggccg acactgagcc cacacagacc
     2641 cagacgctgc tgcggagcct gggggagctg gtcaccggca tctacgtcaa atactggatc
     2701 tatgtgtgcg ccggcatgtt cattgtggtc agcttcgccg gccgcctggt ggtctacaaa
     2761 atcgtctaca tgttcctctt cctgctgtgc ctcaccttgt tccaggtcta ctacaccctg
     2821 tggaggaagc tgctacgtgt cttctggtgg ctcgtggtgg cctatacaat gctcgtgctc
     2881 atcgctgtgt acaccttcca gttccaggac ttccccacct attggcgcaa cctcacgggc
     2941 ttcacagacg agcagttggg cgacctgggc ctggagcagt tcagtgtgtc ggagctcttt
     3001 tccagtatcc tcatccctgg cttcttcctg ctggcctgca tcctgcagct gcactacttc
     3061 cacagaccgt tcatgcagct cactgacctg gagcacgtgc cgccaccagg cacccgccac
     3121 cctcgatggg ctcacaggca ggatgcagtg agcgaggccc ctctgcttga gcatcaggag
     3181 gaagaggaag tcttcaggga agatgggcag agcatggatg ggccccacca ggccacacag
     3241 gtccctgagg gtacggccag caagtggggc ctggtggctg accggctgct ggacctagcg
     3301 gccagcttct cggctgtcct cacccgaatc caggtgttcg ttcggcgctt gctagaactt
     3361 cacgtcttca agctggtggc cctctacact gtctgggtgg ccctgaaaga agtgtctgtg
     3421 atgaacctgc tgctggtggt gctatgggcc ttcgccttgc cctatccgcg cttccggccc
     3481 atggcttcct gcctgtccac cgtgtggacc tgtatcatca ttgtatgcaa gatgctctat
     3541 cagctcaaga ttgtcaaccc gcatgagtac tccagcaact gcactgagcc cttccccaac
     3601 aataccaact tgcagccttt ggagatcaac cagtctttgc tgtaccgtgg ccctgttgac
     3661 cctgccaact ggtttggggt gcggaagggt taccccaact tgggctatat ccagaaccac
     3721 ctgcagatcc ttctgttgct ggtgtttgag gccgtggtgt accggcgcca agagcactac
     3781 cgccggcagc accagcaggc ccctctgccc gcccaggctg tgtgcgcaga tggcacccgc
     3841 cagaggttgg accaggacct acttagctgc ctcaagtatt tcatcaactt cttcttctac
     3901 aaattcgggc tggagatctg cttcttgatg gccgtgaatg tgattgggca gcgtatgaac
     3961 ttcatggtga tcttacacgg ttgctggttg gtggccatcc ttacacgccg gcgccgtgag
     4021 gccatcgccc gcctctggcc taactactgt ctgttcctca cgctgttcct gctgtaccag
     4081 tacctgctgt gtttgggcat gccccctgca ctgtgcattg actatccatg gcgctggagc
     4141 aaggccatcc ccatgaattc cgccctcatc aagtggctgt acctacctga cttcttcaga
     4201 gcccccaact ccaccaacct tatcagtgac ttcctcctgc tgctttgcgc ctcccagcag
     4261 tggcaggtct tctcagcgga gcgaacggag gagtggcaac gcatggcggg catcaacact
     4321 gaccacctgg agcccctgcg tggggagccc aaccctatac ccaacttcat ccactgcagg
     4381 tcctatctgg atatgctgaa ggtggccgtc ttccgctacc tgttctggct ggtgctcgtt
     4441 gtggtgtttg ttgcgggggc cacccgcata agcatcttcg ggctggggta cctgctagcc
     4501 tgcttctacc tgctgctgtt tggcactacc ctgctgcaga aggacacgcg agcccagctc
     4561 gtgctgtggg actgcctcat cctctataat gtcactgtca tcatctctaa gaatatgctg
     4621 tcgctcctgt cctgtgtctt cgtggagcaa atgcagagca acttctgctg ggtcatccag
     4681 ctcttcagcc tcgtgtgcac agtcaaaggc tactatgatc ccaaagagat gatgaccagg
     4741 gaccgggact gcctgctgcc tgtggaggag gccgggatca tctgggacag tatctgcttc
     4801 ttcttcctgc tcttgcaacg gcgcatcttt ctcagccact acttcctgca tgtcagcgct
     4861 gacctgaaag ccacagccct gcaggcatcc aggggctttg ccctctacaa tgcagccaac
     4921 ctgaagagca tcaacttcca tcgccagatt gaggagaagt ccctggccca gctgaaaaga
     4981 cagatgaagc gcatccgtgc caaacaggag aagtacaggc agagccaggc aagtcgtggc
     5041 caactccagt ccaaagaccc tcaggatccc agccaggagc cagggcctga cagcccaggg
     5101 ggctcctccc cgccacggag acagtggtgg cgcccctggc tggaccacgc cacagtcatc
     5161 cactctggcg actacttcct gtttgagtca gatagcgagg aggaagagga ggccctacct
     5221 gaggacccca ggcctgcagc tcagagtgcc ttccagatgg cataccaggc atgggtaacc
     5281 aatgcccaga cagtgctgag gcagcgtcgg gagcgggcac ggcaggagcg ggcagagcag
     5341 ctggcttctg gaggtgactt gaacccagat gtggaaccag tagatgtccc agaagatgag
     5401 atggcaggcc gtagccacat gatgcagcgt gtgctaagca ccatgcagtt cctgtgggtg
     5461 ctgggccagg ccacggtaga cgggctgacg cgctggctgc gtgcattcac gaagcaccac
     5521 cgcaccatga gcgatgtgct gtgcgcagag cgctacctgc tcacccagga gcttcttcgg
     5581 gttggagagg tacgccgagg tgtgctggac cagctttatg tgggtgaaga tgaggccaca
     5641 ttgtcaggtc ccgtggagac ccgggatgga cccagcacag cctcaagtgg gctgggagcc
     5701 gaagagcctt tgagtagcat gacagacgac accagcagcc ccctgagcac aggctataac
     5761 acccgcagtg gcagtgagga gattgtcacc gacgctgggg acctccaggc tgggacctcc
     5821 ctgcacggct cccaagagct tttagccaat gctcgtaccc ggatgcgcac ggccagcgag
     5881 ctgctactgg ataggcgcct gcatatccct gagctggagg aggccgagcg gtttgaggca
     5941 cagcagggcc ggactctgcg gctgctcagg gctgggtacc agtgcgtggc ggcacactcg
     6001 gagctgctct gttacttcat catcatcctt aaccacatgg tgacagcctc ggctgcctcc
     6061 ctggtgctgc ccgtgcttgt gttcctgtgg gccatgctga ccatcccgag gcctagcaag
     6121 cgcttttgga tgacagctat cgtcttcact gaggtcatgg tggtcaccaa atacctgttc
     6181 cagttcggct tcttcccctg gaacagctac gttgtgctgc ggcgctatga gaacaagccc
     6241 tacttccctc cgcgaatcct gggccttgag aaaacggaca gctacatcaa gtatgacctg
     6301 gtgcagctca tggccctctt cttccaccgc tcgcagctac tgtgttatgg cctctgggac
     6361 catgaggagg atcgctatcc caaggaccat tgcaggagta gtgtgaagga ccgggaggcc
     6421 aaggaagagc cagaagctaa gctggaatcg cagtctgaga caggcactgg gcatcccaag
     6481 gagccagtgt tggccggtac tcccagggac cacatccaag ggaaaggaag tattagatcc
     6541 aaggatgtta tccaagatcc cccagaggac cttaagcccc ggcacacgag gcacatcagc
     6601 atacgcttca ggaggcgcaa ggagactcca ggacccaaag gaacagcagt catggagact
     6661 gagcacgagg agggagaagg aaaagaaact acagagagaa agaggccgcg tcacactcaa
     6721 gaaaaatcga agtttcggga gagaatgaag gcagctgggc gccggctgca gagcttctgt
     6781 gtgtcactgg cccagagctt ctaccaaccc ttgcagcgtt tcttccatga cattctgcac
     6841 acaaagtacc gggcggccac cgacgtctac gccctcatgt tcctggccga tattgtcgac
     6901 atcatcatca tcatctttgg tttttgggct tttgggaagc actctgcagc cacagacatt
     6961 gcatcctcgc tgtcagatga ccaggtgcca caggccttcc tgttcatgct gctggtccag
     7021 tttggcacca tggtcatcga ccgtgccctc tacctgcgca agactgtcct gggaaagctg
     7081 gcctttcagg tggtcctggt ggtggcgatt cacatctgga tgttctttat cttaccggct
     7141 gtcactgaga ggatgttcag ccagaatgcg gtggcacagc tgtggtactt cgtcaagtgc
     7201 atttactttg ccctgtccgc ctaccagatc cgctgtggct accccacccg tatcttgggc
     7261 aacttcctca ccaagaaata caaccatcta aacctcttcc ttttccaggg gttccgtcta
     7321 gtgccgttcc tggtggagct gcgggccgtc atggactggg tgtggaccga caccacgctg
     7381 tccctgtcca actggatgtg tgtggaagac atctatgcca acatctttat catcaagtgc
     7441 agccgagaga cagagaagaa atacccccag cccaaggggc agaagaagaa gaaaattgtc
     7501 aagtatggta tgggaggcct cattatcctc ttcctcatcg ccatcatctg gttccctctg
     7561 ctcttcatgt cactgatccg ctctgtggtc ggggtcgtca accagcccat tgatgtcacc
     7621 gtcaccctca agctaggcgg ctatgagcca ctgttcacca tgagcgccca gcagccatcc
     7681 attgtgccat tcacacccca ggcctacgag gagctgtccc agcagtttga cccctatcca
     7741 ctagccatgc agttcattag ccagtacagt cctgaggaca tcgtcactgc acagatcgag
     7801 ggcagctcgg gggcgctgtg gcgcatcagc ccacccagcc gagcccagat gaagcaggag
     7861 ctgtacaacg gcacagccga cattacactg cgctttacct ggaatttcca aagggacctg
     7921 gccaagggtg gcactgtgga gtatactaat gagaagcaca ccttggagct ggcccccaac
     7981 agtacggcac gaaggcagct ggcccaactg ctcgagggca gacctgacca gtcagtggtc
     8041 attccccatc tcttccccaa gtacattcgt gctcccaatg ggcctgaagc caaccctgtg
     8101 aagcagctgc agccagatga ggaagaggac taccttggtg tgcgcatcca gctgcggagg
     8161 gagcaagtgg gcacaggggc ctctggggag caagcgggca ccaaggcctc cgacttcctc
     8221 gagtggtggg tcatcgagct gcaggactgc aaggctgact gcaacctgct gcccatggtc
     8281 atcttcagtg acaaggtcag cccacctagc ctgggcttcc tggccggcta cgggattgtg
     8341 gggctgtacg tctccatcgt gctggtggtt ggcaagtttg tgcggggctt cttcagcgag
     8401 atctctcact ccatcatgtt cgaggaactg ccgtgtgtgg accgcatcct caagctgtgc
     8461 caggacatct tcttggtgcg cgagacccgg gagctggagc tggaggagga gctatacgcc
     8521 aagctcatct tcctgtaccg atctccagag accatgatta agtggacacg tgagagggag
     8581 tagaagcttg gcgcgcctta attaactgca gtcgacggta ccgcgggccc gggatccgcc
     8641 cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
     8701 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
     8761 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
     8821 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
     8881 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
     8941 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
     9001 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg
     9061 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca
     9121 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg
     9181 acgtggtttt cctttgaaaa acacgatgat aatatggcca caaccatggt gagcaagggc
     9241 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     9301 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     9361 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     9421 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     9481 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     9541 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     9601 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     9661 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     9721 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     9781 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     9841 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     9901 accgccgccg ggatcactct cggcatggac gagctgtaca agtaaggccg gccaagctta
     9961 agtttaaacc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
    10021 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
    10081 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
    10141 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
    10201 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
    10261 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
    10321 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
    10381 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
    10441 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
    10501 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
    10561 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
    10621 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
    10681 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
    10741 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
    10801 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
    10861 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
    10921 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
    10981 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
    11041 cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg
    11101 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
    11161 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
    11221 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
    11281 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
    11341 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
    11401 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
    11461 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
    11521 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
    11581 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
    11641 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
    11701 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
    11761 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
    11821 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
    11881 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
    11941 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
    12001 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
    12061 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
    12121 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
    12181 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
    12241 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
    12301 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
    12361 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
    12421 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
    12481 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
    12541 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
    12601 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
    12661 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
    12721 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
    12781 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
    12841 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
    12901 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
    12961 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
    13021 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
    13081 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
    13141 atccggcaaa caaaccaccg ctggtagcgg tttttttgtt tgcaagcagc agattacgcg
    13201 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    13261 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    13321 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    13381 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    13441 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    13501 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
    13561 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    13621 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    13681 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    13741 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    13801 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    13861 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    13921 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    13981 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    14041 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    14101 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    14161 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    14221 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    14281 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    14341 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc
//
