LOCUS Exported 4293 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gene Tagging vector HIS/RFP - Plasmid for monomeric Cherry (mCherry) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091). ACCESSION . VERSION . KEYWORDS GTH-r SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4293) AUTHORS Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S TITLE New families of single integration vectors and gene tagging plasmids for genetic manipulations in budding yeast. JOURNAL Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16. PUBMED 27637489 REFERENCE 2 (bases 1 to 4293) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4293 /organism="synthetic DNA construct" /mol_type="other DNA" promoter complement(88..151) /gene="bla" /label=AmpR promoter promoter 178..196 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(358..376) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 626..645 /label=mCherry-F /note="mCherry, forward primer" terminator 971..1158 /gene="S. cerevisiae ADH1" /label=ADH1 terminator /note="transcription terminator for the S. cerevisiae alcohol dehydrogenase 1 (ADH1) gene" primer_bind complement(1026..1050) /label=pHybLex-R /note="ADH terminator, reverse primer" primer_bind complement(1197..1213) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1197..1213) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" protein_bind complement(1214..1247) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." CDS 1749..2402 /codon_start=1 /gene="Schizosaccharomyces pombe his5" /product="imidazoleglycerol-phosphate dehydratase, required for histidine biosynthesis" /label=S. pombe his5 /note="yeast auxotrophic marker; corresponds to S. cerevisiae HIS3" /translation="MGRRAFVERNTNETKISVAIALDKAPLPEESNFIDELITSKHANQ KGEQVIQVDTGIGFLDHMYHALAKHAGWSLRLYSRGDLIIDDHHTAEDTAIALGIAFKQ AMGNFAGVKRFGHAYCPLDEALSRSVVDLSGRPYAVIDLGLKREKVGELSCEMIPHLLY SFSVAAGITLHVTCLYGSNDHHRAESAFKSLAVAMRAATSLTGSSEVPSTKGVL" terminator 2408..2605 /label=TEF terminator /note="Ashbya gossypii TEF terminator" protein_bind complement(2629..2662) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." promoter 2663..2681 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" promoter complement(2710..2728) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" rep_origin complement(2761..3349) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2841..2860) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(3520..87) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 4143..4162 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" ORIGIN 1 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 61 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 121 tcagggttat tgtctcatga gcggatacat atttgaagta tcgttcgaat taattaaaat 181 taaccctcac taaaggggca tgcgcggccg ctctagaact aaagggcgag gaggataaca 241 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc 301 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca 361 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt 421 tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc 481 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg 541 tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc 601 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg 661 cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc 721 tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga 781 agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca 841 acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg 901 gcatggacga gctgtacaag ggatcttaaa tgctagcatt ttaactcgag ggcgcgccac 961 ttctaaataa gcgaatttct tatgatttat gatttttatt attaaataag ttataaaaaa 1021 aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaaaat tcttattctt 1081 gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagtatgagg tcgctcttat 1141 tgaccacacc tctaccggca gatccgctag ggataacagg gtaatataga tccatggtca 1201 tagctgtttc ctgataactt cgtataatgt atgctatacg aagttatgtc tgtaggcgaa 1261 cctaaccggc caattttcca agaccgctct gccgctgagc ttgcacccgt gccgctacac 1321 aacacccaca catccgtaaa ccccccgcca tcggtatcac caaggttgtc tatgacgagg 1381 cgtacaagag gacgtggggg ccgcctctcc aatgtcacca ataggaaaaa agaaagaccg 1441 agtgcgagtt tctgacagaa gcctcaagaa ttttttttct ttcttcgact atgctggagg 1501 tagagatgat cgaggcagta gtcaagtata tataaattaa ttagttccac caccttcttt 1561 tcttgtgttg ctcctttcga tgctatttct ggctttttct aatttttttc atcgtttctt 1621 actttcttcc taatatataa attcttttgc attttcattc ttcctctcca tttgttcatt 1681 ctccttaaat tttttttagg tctcctttct ttcaagaaac acagtcatcg aactataatt 1741 aactaaacat gggtaggagg gcttttgtag aaagaaatac gaacgaaacg aaaatcagcg 1801 ttgctatcgc tttggacaaa gctcccttac ctgaagagtc gaattttatt gatgaactta 1861 taacttccaa acatgcaaac caaaagggag aacaagtaat ccaagtagac acgggaattg 1921 gattcttgga tcacatgtat catgcactgg ctaaacatgc aggctggagc ttacgacttt 1981 actcaagagg tgatttaatc attgatgatc atcacacagc agaagatact gctattgcac 2041 ttggtattgc attcaagcag gctatgggta actttgccgg cgttaaaaga tttggacatg 2101 cttattgtcc acttgacgaa gctctttcaa gaagcgtagt tgatttgtcg ggacggccct 2161 atgctgttat tgatttggga ttaaagcgtg aaaaggttgg ggaattgtcc tgtgaaatga 2221 tccctcactt actatattcc ttttcggtag ctgctggaat tactttgcat gttacctgct 2281 tatatggtag taatgaccat catcgtgctg aaagcgcttt taaatctctg gctgttgcca 2341 tgcgcgcggc tacaagtctt actggaagtt ctgaagtccc aagcacgaag ggagtgttgt 2401 aatcattact gacaataaaa agattcttgt tttcaagaac ttgtcatttg tatagttttt 2461 ttatattgta gttgttctat tttaatcaaa tgttagcgtg atttatattt tttttcgcct 2521 cgacatcatc tgcccagatg cgaagttaag tgcgcagaaa gtaatatcat gcgtcaatcg 2581 tatgtgaatg ctggtcgcta tactgctgtc gattcgatac taacgccgat aacttcgtat 2641 aatgtatgct atacgaagtt attaatacga ctcactatag ggcgaattgg agctccaatt 2701 cggacgtccc ctatagtgag tcgtattatt aattaattcg aaggccgcgt tgctggcgtt 2761 tttccatagg ctcggccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 2821 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 2881 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 2941 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 3001 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 3061 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 3121 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 3181 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac 3241 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 3301 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 3361 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 3421 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 3481 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 3541 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tgcccgtcgt 3601 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 3661 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 3721 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 3781 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 3841 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 3901 aaggcgagtt acatgatccc ccatgttgtg aaaaaaagcg gttagctcct tcggtcctcc 3961 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 4021 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 4081 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 4141 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 4201 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 4261 tgcacccaac tgatcttcag catcttttac ttt //