LOCUS Exported 17442 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION recipient plasmid [multiplexing]; pICU2:Cas9, FAST, nptII. ACCESSION . VERSION . KEYWORDS pDGE165 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 17442) AUTHORS Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, Boch J, Stuttmann J TITLE Generation of chromosomal deletions in dicotyledonous plants employing a user-friendly genome editing toolkit. JOURNAL Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319. PUBMED 27579989 REFERENCE 2 (bases 1 to 17442) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..17442 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 1062..1691 /codon_start=1 /product="stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 StaA /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" CDS 2125..3192 /codon_start=1 /product="replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 RepA /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV MRYRNLIEGEASAGS" rep_origin 3258..3452 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" primer_bind complement(3688..3710) /label=pGEX 3' /note="pGEX vectors, reverse primer" misc_feature 3796..3936 /label=bom /note="basis of mobility region from pBR322" primer_bind 3851..3870 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(3951..3968) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4122..4710) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4202..4221) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(4954..5745) /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" misc_feature 6266..6290 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" promoter 6436..6615 /label=NOS promoter /note="nopaline synthase promoter" misc_feature 6651..6706 /label=TMV Omega /note="translational enhancer from the tobacco mosaic virus 5'-leader sequence (Gallie et al., 1988)" CDS 6708..7502 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(6762..6781) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 7372..7391 /label=Neo-F /note="Neomycin resistance gene, forward primer" terminator 7515..7767 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" terminator 10138..10845 /label=OCS terminator /note="octopine synthase terminator" CDS complement(10856..10876) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS complement(10889..14992) /codon_start=1 /product="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9 /note="generates RNA-guided double strand breaks in DNA" /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" promoter 15619..15721 /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" CDS 15722..16381 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind complement(15788..15807) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" CDS 16723..17028 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" primer_bind 16907..16926 /label=ccdB-fwd /note="ccdB gene, forward primer" misc_feature 17149..17173 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" ORIGIN 1 ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag tagaatactt gcgactagaa 61 ccggagacat tacgccatga acaagagcgc cgccgctggc ctgctgggct atgcccgcgt 121 cagcaccgac gaccaggact tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac 181 caagctgttt tccgagaaga tcaccggcac caggcgcgac cgcccggagc tggccaggat 241 gcttgaccac ctacgccctg gcgacgttgt gacagtgacc aggctagacc gcctggcccg 301 cagcacccgc gacctactgg acattgccga gcgcatccag gaggccggcg cgggcctgcg 361 tagcctggca gagccgtggg ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt 421 gttcgccggc attgccgagt tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg 481 cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc cctaccctca ccccggcaca 541 gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag aggcggctgc 601 actgcttggc gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac 661 gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc 721 cctggcggcc gccgagaatg aacgccaaga ggaacaagca tgaaaccgca ccaggacggc 781 caggacgaac cgtttttcat taccgaagag atcgaggcgg agatgatcgc ggccgggtac 841 gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct ggccggtttg 901 tctgatgcca agctggcggc ctggccggcc agcttggccg ctgaagaaac cgagcgccgc 961 cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat 1021 atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt atcgctgtac 1081 ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc 1141 aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt gcccgcgatt 1201 gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg 1261 accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga gcgccccagg 1321 cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc 1381 caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag cagcgcattg 1441 aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc 1501 gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt cttgagtccc 1561 gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc gttcttgaat 1621 cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa 1681 aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc 1741 cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc 1801 agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc tgaagatgta 1861 cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg cgcagctacc 1921 agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa ggaggcggca 1981 tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg 2041 gcggttggcc aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc actggaaccc 2101 ccaagcccga ggaatcggcg tgacggtcgc aaaccatccg gcccggtaca aatcggcgcg 2161 gcgctgggtg atgacctggt ggagaagttg aaggccgcgc aggccgccca gcggcaacgc 2221 atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa 2281 gaatcccggc aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc caagggcgac 2341 gagcaaccag attttttcgt tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc 2401 atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc 2461 cgctacgagc ttccagacgg gcacgtagag gtttccgcag ggccggccgg catggccagt 2521 gtgtgggatt acgacctggt actgatggcg gtttcccatc taaccgaatc catgaaccga 2581 taccgggaag ggaagggaga caagcccggc cgcgtgttcc gtccacacgt tgcggacgta 2641 ctcaagttct gccggcgagc cgatggcgga aagcagaaag acgacctggt agaaacctgc 2701 attcggttaa acaccacgca cgttgccatg cagcgtacga agaaggccaa gaacggccgc 2761 ctggtgacgg tatccgaggg tgaagccttg attagccgct acaagatcgt aaagagcgaa 2821 accgggcggc cggagtacat cgagatcgag ctagctgatt ggatgtaccg cgagatcaca 2881 gaaggcaaga acccggacgt gctgacggtt caccccgatt actttttgat cgatcccggc 2941 atcggccgtt ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga agccagatgg 3001 ttgttcaaga cgatctacga acgcagtggc agcgccggag agttcaagaa gttctgtttc 3061 accgtgcgca agctgatcgg gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg 3121 gggcaggctg gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc 3181 gccggttcct aatgtacgga gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt 3241 cgaaaaggtg tctttcctgt ggatagcacg tacattggga acccaaagcc gtacattggg 3301 aaccggaacc cgtacattgg gaacccaaag ccgtacattg ggaaccggtc acacatgtaa 3361 gtgactgata taaaagagaa aaaaggcgat ttttccgcct aaaactcttt aaaacttatt 3421 aaaactctta aaacccgcct ggcctgtgca taactgtctg gccagcgcac agccgaagag 3481 ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg 3541 cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg 3601 cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg caccctgcct 3661 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac 3721 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt 3781 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg 3841 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata 3901 ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact 3961 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 4021 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 4081 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 4141 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 4201 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 4261 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 4321 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 4381 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 4441 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 4501 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 4561 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 4621 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 4681 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 4741 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgcatga tatatctccc 4801 aatttgtgta gggcttatta tgcacgctta aaaataataa aagcagactt gacctgatag 4861 tttggctgtg agcaattatg tgcttagtgc atctaatcgc ttgagttaac gccggcgaag 4921 cggcgtcggc ttgaacgaat ttctagctag acattatttg ccgactacct tggtgatctc 4981 gcctttcacg tagtggacaa attcttccaa ctgatctgcg cgcgaggcca agcgatcttc 5041 ttcttgtcca agataagcct gtctagcttc aagtatgacg ggctgatact gggccggcag 5101 gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg ttactgcgct 5161 gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc agtcgggcgg 5221 cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt caggaaccgg 5281 atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc ttgcttttgt 5341 cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg caagaatgtc 5401 attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc acggaatgat 5461 gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct ctccagggga 5521 agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat caagccttac 5581 ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat ccactgcgga 5641 gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac 5701 ctctgatagt tgagtcgata cttcggcgat caccgcttcc cccatgatgt ttaactttgt 5761 tttagggcga ctgccctgct gcgtaacatc gttgctgctc cataacatca aacatcgacc 5821 cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtaccc caaaaaaaca 5881 gtcataacaa gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt 5941 tctggaccag ttgcgtgagc gcatacgcta cttgcattac agcttacgaa ccgaacaggc 6001 ttatgtccac tgggttcgtg cccgaattga tcacaggcag caacgctctg tcatcgttac 6061 aatcagcatg ctaccctccg cgagatcatc cgtgtttaaa acccggcagc ttagttgccg 6121 ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc 6181 tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc 6241 ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc 6301 ttagacaact taataacaca ttgcggatcc ctcggtcaca tgtgcatcct ctctgggaga 6361 gcggagaatt aagggagtca cgttatgacc cccgccgatg acgcgggaca agccgtttta 6421 cgtttggaac tgacagaacc gcaacgttga aggagccact cagccgcggg tttctggagt 6481 ttaatgagct aagcacatac gtcagaaacc attattgcgc gttcaaaagt cgcctaaggt 6541 cactatcagc tagcaaatat ttcttgtcaa aaatgctcca ctgacgttcc ataaattccc 6601 ctcggtatcc aattagagtc tcatattcac tctcatactg tatttttaca acaattacca 6661 acaacaacaa acaacaaaca acattacaat tactatttac aattacaatg attgaacaag 6721 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg 6781 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc 6841 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag 6901 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca 6961 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat 7021 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata 7081 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac 7141 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc 7201 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gatgatctcg 7261 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg 7321 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta 7381 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg 7441 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct 7501 gagcttgtca agcagatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg 7561 ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa 7621 ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat 7681 tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc 7741 gcgcggtgtc atctatgtta ctagatcgac gctccatggg aattcgtaaa gcgactaaat 7801 ggagcaacct actgtttttg aagtttgggt tgccatgtaa tcacttgcaa taaatatatg 7861 tactaatgac tgcatcggct tgcgtgtcta aatttgtagc ttattgaaaa ctttttgtgg 7921 ttaaagacga atccagttaa ctaacataag tttaacaaaa caagaccttt tccatatata 7981 gaccaccgtt accaacttac aaagaagcca aataaagtac aacacaacac ttgcaagtta 8041 caaaaagaga ggaaaaaaaa catggtacca tttgacaaca tatagaaaca cacataaaca 8101 ttacttataa caaaaagatt caaactcggc ttttattatc tccccctgat caaattctaa 8161 acactttatt cctcataaac taactccccg acattggagt tattggacta tgacgatgac 8221 atcagtgggg taagctcact tgtgccccag tttgctaggg aggtcgcagt atctggccac 8281 agccacctcg tgctgctcga cgtaggtttc tttgtcggcc tccttgattc tttccagtct 8341 gtggtccaca tagtagacgc cgggcatctt gaggttctta gcgggtttct tggatctgta 8401 tgtggtcttg aagttgcaga tcaggtggcc cccgcccacg agcttcaggg ccatgtcgct 8461 tctgccttcc aggccgccgt cagcggggta cagcatctcg gtgttggcct cccagccgag 8521 tgttttcttc tgcatcacag ggccgttgga tgggaagttc acccctctga tcttgacgtt 8581 gtagatgagg cagccgtcct ggaggctggt gtcctgggta gcggtcagca cgcccccatc 8641 ttcgtatgtg gtgactctct cccatgtgaa gccctcaggg aaggactgct taaagaagtc 8701 ggggatgccc tgggtgtggt tgatgaaggt tctgctgccg tacatgaagc tggtagccag 8761 gatgtcgaag gcgaagggga gagggccgcc ctcgaccacc ttgattctca tggtctgggt 8821 gccctcgtag ggcttgcctt cgccctcgga tgtgcacttg aagtggtggt tgttcacggt 8881 gccctccatg tacagcttca tgtgcatgtt ctccttaatc agctcgctca tagtagtgtg 8941 ctggccacca cgagtacggt cacggtcatg ttccccacca gtatgttgct gtccgtagta 9001 ctgagctctg tctttcagat cctgagcttt gcttcccaac ttcatccttg cactgtccaa 9061 cttgtctgat ccctgtgggt gctctcccgt tgcgtaccta ttcacaatca ataggtatca 9121 taccaagcat tttacctata tagatgatat acacagacac attcaagcta gctaggtcaa 9181 atttcgtatt tagttaacca aatgttccct agaatttgca atttcttatt gttacatttg 9241 ttattcgacc aaaaaaattg atccaaagct actggctcaa cacatgcatg cacatattgc 9301 acaaaattat gaagtaagat gataaatgtg tgcttacttg taaatccaag agaaaacggt 9361 tatagcggca atgccaaacc ctccagagga aagaaaaccg gtgatgagga gtgcaactgt 9421 gatgagagcc gggacaagga ttgggctgaa gataacgagc agaggtgttg caacagtcaa 9481 agctatgaca gttccaacaa gggtaaggct ggagagaaca aggagggaac caccagctgt 9541 gacagcagtt gcagctttag caatctgcct agacttggag tagtcagatc ctcgtccgga 9601 catctggtac tggtcacgat ctcggcccat catcgggtac tgatctctgc cgatgatatc 9661 gtgatgggtt cctctagctg tatccgccat ttttttgttc ttgtttacta gagagaatga 9721 gattttgaat gagagattga tcaaaaagat gtgtatatat tttggaagag gtaggagtgt 9781 ttgttagata tattaagaaa ggagacgaga agtcacgtgt caatggagac atcatgcaat 9841 tgtaggccac gtgttaatgg cgtcaccatg cagccgctgc atgtgttaac gagaggagtc 9901 tattcaaaga cacgtttgtt ttttgcctag ctcagaatgt ggcgttggca tcgaaccgaa 9961 acataaaacg caaaacgaac ctttgggctc gtcagtatgg gtttgggcct tcaatagatt 10021 ccttctatac ggcccacata tccactattg gcaacaaaat tagaaaaatt taggatttag 10081 ttttaaaatt tgttccgcga cattctagaa ctccgagctc gaattctagt agcggtcctg 10141 ctgagcctcg acatgttgtc gcaaaattcg ccctggaccc gcccaacgat ttgtcgtcac 10201 tgtcaaggtt tgacctgcac ttcatttggg gcccacatac accaaaaaaa tgctgcataa 10261 ttctcggggc agcaagtcgg ttacccggcc gccgtgctgg accgggttga atggtgcccg 10321 taactttcgg tagagcggac ggccaatact caacttcaag gaatctcacc catgcgcgcc 10381 ggcggggaac cggagttccc ttcagtgagc gttattagtt cgccgctcgg tgtgtcgtag 10441 atactagccc ctggggcact tttgaaattt gaataagatt tatgtaatca gtcttttagg 10501 tttgaccggt tctgccgctt tttttaaaat tggatttgta ataataaaac gcaattgttt 10561 gttattgtgg cgctctatca tagatgtcgc tataaaccta ttcagcacaa tatattgttt 10621 tcattttaat attgtacata taagtagtag ggtacaatca gtaaattgaa cggagaatat 10681 tattcataaa aatacgatag taacgggtga tatattcatt agaatgaacc gaaaccggcg 10741 gtaaggatct gagctacaca tgctcaggtt ttttacaacg tgcacaacag aattgaaagc 10801 aaatatcatg cgatcatagg cttctcgcat atctcattaa agcaggacaa gctcacacct 10861 tcctcttctt cttggggtca gccctgctgt ctccaccgag ctgagagagg tcgattcttg 10921 tttcatagag ccccgtaatt gactgatgaa tcagtgtggc gtccaggacc tcctttgtag 10981 aggtgtaccg ctttctgtct atggtggtgt cgaagtactt gaaggctgca ggcgcgccca 11041 agttggtcag agtaaacaag tggataatgt tttctgcctg ctccctgatg ggcttatccc 11101 tgtgcttatt gtaagcagaa agcaccttat cgaggttagc gtcggcgagg atcactcttt 11161 tggagaattc gcttatttgc tcgatgatct catcaaggta gtgtttgtgt tgttccacga 11221 acagctgctt ctgctcatta tcttcgggag atcctttgag cttttcatag tggctggcca 11281 gatacaagaa attaacgtat ttagagggca gtgccagctc gttacctttc tgcagctcgc 11341 ccgcactagc gagcattcgt ttccggccgt tttcaagctc aaagagagag tacttgggaa 11401 gcttaatgat gaggtctttt ttgacctctt tatatccttt cgcctcgaga aagtcgatgg 11461 ggtttttttc gaagcttgat cgctccatga ttgtgatgcc cagcagttcc ttgacgcttt 11521 tgagtttttt agacttccct ttctccactt tggccacaac cagtacactg taagcgactg 11581 taggagaatc gaatccgccg tatttcttgg ggtcccaatc ttttttgcgt gcgatcagct 11641 tgtcgctgtt ccttttcggg aggatacttt ccttggagaa gcctccggtc tgtacttcgg 11701 tctttttaac gatgttcacc tgcggcatgg acaggacctt ccggactgtc gcgaaatccc 11761 tacccttgtc ccacacgatt tctcctgttt ctccgtttgt ttcgataagt ggtcgcttcc 11821 gaatctctcc attggccagt gtaatctcgg tcttgaaaaa attcataata ttgctgtaaa 11881 agaagtactt agcggtggcc ttgcctattt cctgctcaga ctttgcgatc attttcctaa 11941 catcgtacac tttatagtct ccgtaaacaa attcagattc aagcttggga tattttttga 12001 taagtgcagt gcctaccact gcattcaggt aggcatcatg cgcatggtgg taattgttga 12061 tctctctcac cttataaaac tgaaagtcct ttctgaaatc tgaaaccagc ttagacttca 12121 gagtaataac tttcacctct cgaatcagtt tgtcattttc atcgtacttg gtgttcatgc 12181 gtgaatcgag aatttgggcc acgtgcttgg tgatctggcg tgtctcaaca agctgccttt 12241 tgatgaagcc ggctttatcc aactcagaca ggccacctcg ttcagcctta gtcagattat 12301 cgaacttccg ttgtgtgatc agtttggcgt tcagcagctg ccgccaataa tttttcattt 12361 tcttgacaac ttcttctgag gggacgttat cactcttccc tctattttta tcggatcttg 12421 tcaacacttt attatcaata gaatcatctt tgagaaaaga ctggggcacg atatgatcca 12481 cgtcgtagtc ggagagccga ttgatgtcca gttcctgatc cacgtacatg tccctgccgt 12541 tctgcaggta gtacaggtag agcttctcat tctgaagctg ggtgttttca actgggtgtt 12601 ccttaaggat ttgggacccc agttctttta taccctcttc aatcctcttc atcctttccc 12661 tactgttctt ctgtcccttc tgggtagttt ggttctctcg ggccatctcg ataacgatat 12721 tctcgggctt atgccttccc attactttga cgagttcatc cacgacctta acggtctgca 12781 gtattccctt tttgatagct gggctacctg caagattagc gatgtgctcg tggagactgt 12841 ccccctggcc agaaacttgt gctttctgga tgtcctcctt aaaggtgaga gagtcatcat 12901 ggatcaactg catgaagttc cggttggcaa atccatcgga cttaagaaaa tccaggattg 12961 tctttccact ctgcttgtct cggatcccat tgatcagttt tcttgacagc cgcccccatc 13021 ctgtatatcg gcgcctcttg agctgtttca tgactttgtc gtcgaagaga tgagcgtaag 13081 ttttcaagcg ttcttcaatc atctccctat cttcaaacaa cgtaagggtg aggacaatgt 13141 cctcaagaat gtcctcgttc tcctcattgt ccaggaagtc cttgtcttta atgattttca 13201 ggagatcgtg atacgttccc agggatgcgt tgaagcgatc ctccactccg ctgatttcaa 13261 cagagtcgaa acattcaatc tttttgaaat aatcttcttt gagctgtttc acggtaactt 13321 tccggttcgt cttgaagagg aggtccacga tagctttctt ctgctctcca gacaggaatg 13381 ctggctttct catcccttct gtgacgtatt tgaccttggt gagctcgtta taaactgtga 13441 agtactcgta cagcagagag tgtttaggaa gcaccttttc gttaggcaga tttttatcaa 13501 agttagtcat cctttcgatg aaggactggg cagaggcccc cttatccacg acttcctcga 13561 agttccaggg agtgatagtc tcttctgatt tgcgagtcat ccacgcgaat ctggaatttc 13621 cccgggcgag ggggcctaca tagtagggta tccgaaatgt gaggattttc tcaatctttt 13681 ccctgttatc tttcaaaaag gggtagaaat cctcttgccg cctgaggata gcgtgcagtt 13741 cgcccaggtg aatctggtgg gggatgcttc cattgtcgaa agtgcgctgt ttgcgcaaca 13801 gatcttctct gttaagcttt accagcagct cctcggtgcc gtccattttt tccaagatgg 13861 gcttaataaa tttgtaaaat tcctcctggc ttgctccgcc gtcaatgtat ccggcgtagc 13921 catttttaga ctgatcgaag aaaatttcct tgtacttctc aggcagttgc tgtctgacaa 13981 gggccttcag caaagtcaag tcttggtggt gctcatcata gcgcttgatc atactagcgc 14041 tcagcggagc tttggtgatc tccgtgttca ctcgcagaat atcactcagc agaatggcgt 14101 ctgacaggtt ctttgccgcc aaaaaaaggt ctgcgtactg gtcgccgatc tgggccagca 14161 gattgtcgag atcatcatcg taggtgtctt tgctcagttg aagcttggca tcttcggcca 14221 ggtcgaagtt agatttaaag ttgggggtca gcccgagtga cagggcgata agattaccaa 14281 acaggccgtt cttcttctcc ccagggagct gtgcgatgag gttttcgagc cgccgggatt 14341 tggacagcct agcgctcagg attgctttgg cgtcaactcc ggatgcgttg atcgggttct 14401 cttcgaaaag ctgattgtaa gtctgaacca gttggataaa gagtttgtcg acatcgctgt 14461 tgtctgggtt caggtccccc tcgatgagga agtgtccccg aaatttgatc atatgcgcca 14521 gcgcgagata gatcaaccgc aagtcagcct tatcagtact gtctacaagc ttcttcctca 14581 gatgatatat ggttgggtac ttttcatggt acgccacctc gtccacgata ttgccaaaga 14641 ttgggtggcg ctcgtgcttt ttatcctcct ccaccaaaaa ggactcctcc agcctatgga 14701 agaaagagtc atccacctta gccatctcat tactaaagat ctcctgcagg tagcagatcc 14761 gattctttct gcgggtatat ctgcgccgtg ctgttctttt gagccgcgtg gcttcggccg 14821 tctccccgga gtcgaacagg agggcgccaa tgaggttctt ctttatgctg tggcgatcgg 14881 tattgcccag aactttgaat tttttgctcg gcaccttgta ctcgtccgta atgacggccc 14941 agccgacgct gtttgtgccg atatcgagcc caatggagta cttcttgtcc atttttacaa 15001 atccggtcaa tttggtggtt tggtgaagct gagtaactga tttaggttta agaacccaga 15061 ttaagaaatg gagatggcgg gaaaatcgac acgattttgg cgctatccat ttgccaaatc 15121 cgaatccaac aaacaaacgg gttctcttaa tgggccttac tcctctttaa taaagcccaa 15181 aaacaaaaac aaaaaagaag caattatgag acatctattt gaacgagatc aagcatcaga 15241 gagttgtagg agctacctcg gtctgtgaag taattgctga tggagtccac caagtgtaga 15301 atctagctaa atacagtaaa agaatcaaag tttcagcctg agttctaagt tactgatatt 15361 taccgattcg aaagtctaaa ctattgctga aactactaat aagttcatat ccaaaatcat 15421 tgttctctat gataaagccc aattgaaaga gaaaagaaaa aaaaacaatg atgagagatg 15481 atgtgttgcc gaccacctaa aaccacttta acaaaagcaa aagccaagta aaggttacat 15541 tctctgcaac ttttttgtca taataaaaac taaacatcta ttaactccaa gcttgaattc 15601 ttgcagagac cgagacgttg atcggcacgt aagaggttcc aactttcacc ataatgaaat 15661 aagatcacta ccgggcgtat tttttgagtt atcgagattt tcaggagcta aggaagctaa 15721 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga 15781 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga 15841 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat 15901 tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg 15961 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga 16021 aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata 16081 ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga 16141 gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt 16201 ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg 16261 cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtctgtg atggcttcca 16321 tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta 16381 atcgcgtgga tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt 16441 ttgcggtata agaatatata ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct 16501 atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct caaggcatat 16561 atgatgtcaa tatctccggt ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc 16621 gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc cggtttattg 16681 aaatgaacgg ctcttttgct gacgagaaca ggggctggtg aaatgcagtt taaggtttac 16741 acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac 16801 acgcccgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc 16861 tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc 16921 gatatggcca gtgtgccggt ttccgttatc ggggaagaag tggctgatct cagccaccgc 16981 gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggctcc 17041 cttatacaca gccagtcggt ctcagcttct gcaggcatgc gacgtcgagc ttagcttgag 17101 cttggatcag attgtcgttt cccgccttca gtttaaacta tcagtgtttg acaggatata 17161 ttggcgggta aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc 17221 gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg 17281 ggatcaaagt actttaaagt actttaaagt actttaaagt actttgatcc aacccctccg 17341 ctgctatagt gcagtcggct tctgacgttc agtgcagccg tgttctgaaa acgacatgtc 17401 gcacaagtcc taagttacgc gacaggctgc cgccctgccc tt //