LOCUS       Exported               10943 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    hIRE1a wt
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10943)
  AUTHORS   Lipson KL, Ghosh R, Urano F
  TITLE     The role of IRE1alpha in the degradation of insulin mRNA in 
            pancreatic beta-cells.
  JOURNAL   PLoS ONE. 2008  . 3(2):e1648.
  PUBMED    18286202
REFERENCE   2  (bases 1 to 10943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10943
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     240..260
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        242..260
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        288..514
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             515..695
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    742..867
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1360..1593
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1778..1822
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2089..2205
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2228..2531
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2532..2735
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2686..2706
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2737..2755
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2758..2776
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2817..2841
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5835..5859)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5890..6478
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5943..5963)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6361..6372)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6492..6991
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6527..6550)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6924..6943
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7012..7611
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7012..7031)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7508..7528
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7749..7982
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8054..8175
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8091..8110)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8145..8164
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8215..8350
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8277..8296
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(8370..8389)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8371..8389)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8399..8416)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8399..8415)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8408..8430)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8557..9012
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8644..8663)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8854..8875
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9038..9142
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9143..10003
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9361..9380)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10174..10762
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10663..10682
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10916..10933
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
      241 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
      301 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
      361 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
      421 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
      481 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
      541 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      601 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      661 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      721 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      781 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      841 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      901 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      961 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     1021 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1081 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1141 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1201 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1261 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1321 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1381 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1441 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1501 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1561 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1621 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1681 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1741 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1801 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1861 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1921 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1981 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     2041 agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt ttaaaagaaa
     2101 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2161 acaaactaaa gaattacaaa aacaaattac aaaattcaaa attttatcga taagcttggg
     2221 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     2281 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     2341 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     2401 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     2461 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     2521 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
     2581 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     2641 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
     2701 ggcgtgtacg gtgggaggtc tatataagca gagctctccc tatcagtgat agagatctcc
     2761 ctatcagtga tagagatcgt cgactagtcc agtgtggtgg aattctgcag atatcaacaa
     2821 gtttgtacaa aaaagcaggc tttaaaggaa ccaattcagt cgactggatc cggtaccgaa
     2881 ttcgccatgc cggcccggcg gctgctgctg ctgctgacgc tgctgctgcc cggcctcggg
     2941 atttttggaa gtaccagcac agtgacgctt cctgaaacct tgttgtttgt gtcaacgctg
     3001 gatggaagtt tgcatgctgt cagcaagagg acaggctcaa tcaaatggac tttaaaagaa
     3061 gatccagtcc tgcaggtccc aacacatgtg gaagagcctg cctttctccc agatcctaat
     3121 gatggcagcc tgtatacgct tggaagcaag aataatgaag gcctgacgaa acttcctttt
     3181 accatcccag aattggtgca ggcatcccca tgccgaagtt cagatggaat cctctacatg
     3241 ggtaaaaagc aggacatctg gtatgttatt gacctcctga ccggagagaa gcagcagact
     3301 ttgtcatcgg cctttgcaga tagtctctgc ccatcaacct ctcttctgta tcttgggcga
     3361 acagaataca ccatcaccat gtacgacacc aaaacccgag agctccggtg gaatgccacc
     3421 tactttgact atgcggcctc actgcctgag gacgacgtgg actacaagat gtcccacttt
     3481 gtgtccaatg gtgatgggct ggtggtgact gtggacagtg aatctgggga cgtcctgtgg
     3541 atccaaaact acgcctcccc tgtggtggcc ttttatgtct ggcagcggga gggtctgagg
     3601 aaggtgatgc acatcaatgt cgctgtggag accctgcgct atctgacctt catgtctggg
     3661 gaggtggggc gcatcacaaa gtggaagtac ccgttcccca aggagacaga ggccaagagc
     3721 aagctgacgc ccactctgta tgttgggaaa tactctacca gcctctatgc ctctccctca
     3781 atggtacacg agggggttgc tgtcgtgccc cgcggcagca cacttccttt gctggaaggg
     3841 ccccagactg atggcgtcac catcggggac aagggggagt gtgtgatcac gcccagcacg
     3901 gacgtcaagt ttgatcccgg actcaaaagc aagaacaagc tcaactactt gaggaattac
     3961 tggcttctga taggacacca tgaaacccca ctgtctgcgt ctaccaagat gctggagaga
     4021 tttcccaaca atctacccaa acatcgggaa aatgtgattc ctgctgattc agagaaaaag
     4081 agctttgagg aagttatcaa cctggttgac cagacttcag aaaacgcacc taccaccgtg
     4141 tctcgggatg tggaggagaa gcccgcccat gcccctgccc ggcccgaggc ccccgtggac
     4201 tccatgctta aggacatggc taccatcatc ctgagcacct tcctgctgat tggctgggtg
     4261 gccttcatca tcacctatcc cctgagcatg catcagcagc agcagctcca gcaccagcag
     4321 ttccagaagg aactggagaa gatccagctc ctgcagcagc agcagcagca gctgcccttc
     4381 cacccacctg gagacacggc tcaggacggc gagctcctgg acacgtctgg cccgtactca
     4441 gagagctcgg gcaccagcag ccccagcacg tcccccaggg cctccaacca ctcgctctgc
     4501 tccggcagct ctgcctccaa ggctggcagc agcccctccc tggaacaaga cgatggagat
     4561 gaggaaacca gcgtggtgat agttgggaaa atttccttct gtcccaagga tgtcctgggc
     4621 catggagctg agggcacaat tgtgtaccgg ggcatgtttg acaaccgcga cgtggccgtg
     4681 aagaggatcc tccccgagtg ttttagcttc gcagaccgtg aggtccagct gttgcgagaa
     4741 tcggatgagc acccgaacgt gatccgctac ttctgcacgg agaaggaccg gcaattccag
     4801 tacattgcca tcgagctgtg tgcagccacc ctgcaagagt atgtggagca gaaggacttt
     4861 gcgcatctcg gcctggagcc catcaccttg ctgcagcaga ccacctcggg cctggcccac
     4921 ctccactccc tcaacatcgt tcacagagac ctaaagccac acaacatcct catatccatg
     4981 cccaatgcac acggcaagat caaggccatg atctccgact ttggcctctg caagaagctg
     5041 gcagtgggca gacacagttt cagccgccga tctggggtgc ctggcacaga aggctggatc
     5101 gctccagaga tgctgagcga agactgtaag gagaacccta cctacacggt ggacatcttt
     5161 tctgcaggct gcgtctttta ctacgtaatc tctgagggca gccacccttt tggcaagtcc
     5221 ctgcagcggc aggccaacat cctcctgggt gcctgcagcc ttgactgctt gcacccagag
     5281 aagcacgaag acgtcattgc acgtgaattg atagagaaga tgattgcgat ggatcctcag
     5341 aaacgcccct cagcgaagca cgtgctcaaa cacccgttct tctggagcct agagaagcag
     5401 ctccagttct tccaggacgt gagcgacaga atagaaaagg aatccctgga tggcccgatc
     5461 gtgaagcagt tagagagagg cgggagagcc gtggtgaaga tggactggcg ggagaacatc
     5521 actgtccccc tccagacaga cctgcgtaaa ttcaggacct ataaaggtgg ttctgtcaga
     5581 gatctcctcc gagccatgag aaataagaag caccactacc gggagctgcc tgcagaggtg
     5641 cgggagacgc tggggtccct ccccgacgac ttcgtgtgct acttcacgtc tcgcttcccc
     5701 cacctcctcg cacacaccta ccgggccatg gagctgtgca gccacgagag actcttccag
     5761 ccctactact tccacgagcc cccagagccc cagcccccag tgactccaga cgccctctga
     5821 ctcgagatat ctagacccag ctttcttgta caaagtggtt gatatccagc acagtggcgg
     5881 ccgctcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     5941 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     6001 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     6061 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     6121 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     6181 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     6241 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     6301 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     6361 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     6421 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     6481 ggaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc
     6541 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc
     6601 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc
     6661 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat
     6721 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg
     6781 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag
     6841 gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc
     6901 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct
     6961 gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac catgaccgag
     7021 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     7081 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     7141 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     7201 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     7261 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     7321 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     7381 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     7441 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     7501 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     7561 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc
     7621 cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atgcatctcg agggcccggt
     7681 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     7741 ggggggactg gaagggctag ctcactccca acgaagacaa gatctgcttt ttgcttgtac
     7801 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     7861 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     7921 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     7981 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     8041 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     8101 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     8161 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
     8221 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     8281 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     8341 tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
     8401 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     8461 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     8521 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
     8581 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     8641 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     8701 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     8761 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     8821 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     8881 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     8941 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     9001 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     9061 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     9121 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     9181 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     9241 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     9301 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     9361 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     9421 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     9481 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     9541 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     9601 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     9661 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     9721 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     9781 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     9841 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     9901 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     9961 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
    10021 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
    10081 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
    10141 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
    10201 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
    10261 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
    10321 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
    10381 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
    10441 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
    10501 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
    10561 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
    10621 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
    10681 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
    10741 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
    10801 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
    10861 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
    10921 gtcagtgagc gaggaagcgg aag
//
