LOCUS Exported 839 bp ds-DNA linear SYN 22-MAY-2021 DEFINITION Allows for expression of an sgRNA in Toxoplasma gondii and confers resistance to pyrimethamine. ACCESSION . VERSION . KEYWORDS pU6-DHFR SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 839) AUTHORS Sidik SM, Huet D, Ganesan SM, Huynh MH, Wang T, Nasamu AS, Thiru P, Saeij JP, Carruthers VB, Niles JC, Lourido S TITLE A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. JOURNAL Cell. 2016 Sep 8;166(6):1423-1435.e12. doi: 10.1016/j.cell.2016.08.019. Epub 2016 Sep 2. PUBMED 27594426 REFERENCE 2 (bases 1 to 839) AUTHORS . TITLE Direct Submission JOURNAL Exported May 22, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..839 /organism="synthetic DNA construct" /mol_type="other DNA" ORIGIN 1 cgggaggccg ttgttgatgc cgatgcccct cttgggggtc atcgcgacga ccagacacac 61 cggtttctgc atcttcccag acacgacaac gccccgtaga gcagaaacgc actactaaag 121 cgaaacttca cccgtccctg ctgcactcag agcagtgctc cgcactgccg tgtggtaaaa 181 tgaaaaggtt ctacgagaca cgcgtctccg gatcgacaag cgaaggatct gcacacctgc 241 tctcgatgtc gaacaaagca cggaggagag acggaaagtg cttacatcga acacggttat 301 caaacccgag aaaaagaaac gaacagaaga aaaaggaaac ctccgcatac ttttaaagaa 361 tgaagttccc cgattttccc aaaaatggcg tcattttcgc gcacggcagt cagataacag 421 gtgtagcggc tgcccaccaa cagagacggc gcggccgaca ggacgctact gggactgcga 481 acagcagcaa gatcggatct tccgcgggcg ggtttgaatg caaggtttcg tgctgtcgaa 541 aaagagaatg gaacttaatg tccacgtagt tcgcgcatga aaagcgtcct accactcaaa 601 aaagacaaaa accgcactcg cggcgttgaa tgtgcggggg aacggatggc tctctttttt 661 ggaaagcgaa tgcaaaaaga caagacgcag acgcatacaa cgttagtccc ctggcacgag 721 agatagaggt gctgggggcc attgcggtgt cgtggattta ccagtcatgg acgagatcgt 781 tccggagcct gtctttcctg aaagtctgaa aaaattgcgg gaaagtcacg catatggaa //