LOCUS       Exported                 589 bp ds-DNA     linear   SYN 15-JUN-2021
DEFINITION  piggyBac transposon for dox-inducible expression of the MKOS 
            polycistronic reprogramming cassette (with mCherry).
ACCESSION   .
VERSION     .
KEYWORDS    PB-TAC-MKOS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Kim SI, Oceguera-Yanez F, Hirohata R, Linker S, Okita K, Yamada Y, 
            Yamamoto T, Yamanaka S, Woltjen K
  TITLE     KLF4 N-terminal variance modulates induced reprogramming to 
            pluripotency.
  JOURNAL   Stem Cell Reports. 2015 Apr 14;4(4):727-43. doi: 
            10.1016/j.stemcr.2015.02.004. Epub 2015 Mar 12.
  PUBMED    25772473
REFERENCE   2  (bases 1 to 589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 15, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(58..111)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 cccacctggt gggggtgaga aggcgaagtc tgaagccagg tgtccagcca tctcgagtgg
       61 gccaggattc tcctcgacgt caccgcatgt tagcagactt cctctgccct ctccggagcc
      121 aaagtgcctc ttcatgtgta aggcaaggtg gtccgacctg gaaaaggccc tgtcgcactt
      181 ctggcactga aagggccggt gccctgtgtg tttgcggtag tgcctggtca gttcatcgga
      241 gcgggcgaat ttccacccac agccgtccca gtcacagtgg taaggtttct cgcctgtgtg
      301 agttcgcagg tgtgccttga gatgagaact cttggtatag gttttgccac agcctgcata
      361 gtcacaagtg tgggtggctg ttcttttccg gggccacgac cttcttcccc tctttggctt
      421 gggctcctct ggcaggcagg aacccggtgg catgagctct tgataatgga gagaggggac
      481 ttgtgactgc atctggtctg gcaggaaagg agggtagttg ggccccggat ggggatggaa
      541 tcctggggga agaggcaggc cagggtgaca gtccctgctg ttcagcagt
//
