LOCUS       Exported                 469 bp ds-DNA     linear   SYN 15-JUN-2021
DEFINITION  piggyBac transposon for dox-inducible expression of the MKOS 
            polycistronic reprogramming cassette (with mCherry).
ACCESSION   .
VERSION     .
KEYWORDS    PB-TAC-MKOS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Kim SI, Oceguera-Yanez F, Hirohata R, Linker S, Okita K, Yamada Y, 
            Yamamoto T, Yamanaka S, Woltjen K
  TITLE     KLF4 N-terminal variance modulates induced reprogramming to 
            pluripotency.
  JOURNAL   Stem Cell Reports. 2015 Apr 14;4(4):727-43. doi: 
            10.1016/j.stemcr.2015.02.004. Epub 2015 Mar 12.
  PUBMED    25772473
REFERENCE   2  (bases 1 to 469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 15, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(67..126)
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
ORIGIN
        1 gccgccgccc cccgaagctt gctgcgggcc cggcggcttc agctccgtct ccatcatgtt
       61 atacatagga ccggggttac tttcaacatc gccagcgagt ttcaacaaag cgtagttagt
      121 acattgaccc gacccgtttg aatgcatggg agagcccaga gcagtgacgg gaacagaggg
      181 aaaggcctcg ccctcaggaa aagggactga gtagagtgtg gtgaagtggg ggcttccata
      241 gcctggggtg ccaaagtggg gacctggggg cagaggaaag gatacagccc cccctgggaa
      301 aggtgtccct gtagcctcat actcttctcg ttgggaatac tcaatacttg atcttttgcc
      361 cttctggcgc cggttacaga accatactcg aaccacatcc ttctctagcc caagctgatt
      421 ggcgatgtga gtgatctgct gtagggaggg cttcgggcac ttcagaaac
//
