LOCUS       Exported               11233 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  piggyBac transposon for dox-inducible expression of the MKOS 
            polycistronic reprogramming cassette (with mCherry).
ACCESSION   .
VERSION     .
KEYWORDS    PB-TAC-MKOS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11233)
  AUTHORS   Kim SI, Oceguera-Yanez F, Hirohata R, Linker S, Okita K, Yamada Y, 
            Yamamoto T, Yamanaka S, Woltjen K
  TITLE     KLF4 N-terminal variance modulates induced reprogramming to 
            pluripotency.
  JOURNAL   Stem Cell Reports. 2015 Apr 14;4(4):727-43. doi: 
            10.1016/j.stemcr.2015.02.004. Epub 2015 Mar 12.
  PUBMED    25772473
REFERENCE   2  (bases 1 to 11233)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11233
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     588..610
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     602..619
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   711..773
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    1145..1163
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1182..1200
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1219..1237
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1256..1274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1293..1311
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1330..1348
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1367..1385
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1404..1422
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1444..1468
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    1548..1572
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2945..3010
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             4469..4522
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5594..5653
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     protein_bind    complement(6643..6667)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6709..7295
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6876..6893)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7103..7122
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7296..8006
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7441..7459)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7709..7728
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(8018..8037)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8083..8138
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8137..8156)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     repeat_region   8858..8892
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(9042..9060)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9081..9097)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9081..9097)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9094..9116)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9105..9121
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9129..9159)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9174..9195
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9312..9329)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9483..10071)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9563..9582)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10242..11102)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10865..10884
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11103..11207)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg cgccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcgg
      661 tattcacgac agcaggctga ataataaaaa aattagaaac tattatttaa ccctagaaag
      721 ataatcatat tgtgacgtac gttaaagata atcatgcgta aaattgacgc atgtgtttta
      781 tcggtctgta tatcgaggtt tatttattaa tttgaataga tattaagttt tattatattt
      841 acacttacat actaataata aattcaacaa acaatttatt tatgtttatt tatttattaa
      901 aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa aacttttaaa cattctctct
      961 tttacaaaaa taaacttatt ttgtacttta aaaacagtca tgttgtatta taaaataagt
     1021 aattagctta acctatacat aatagaaaca aattatactt attagtcagt cagaaacaac
     1081 tttggcacat atcaatatta tgctctcgtt aattaactcg agggatccaa gctggccgca
     1141 cgtctcccta tcagtgatag agaagtcgac acgtctcgag ctccctatca gtgatagaga
     1201 aggtacgtct agaacgtctc cctatcagtg atagagaagt cgacacgtct cgagctccct
     1261 atcagtgata gagaaggtac gtctagaacg tctccctatc agtgatagag aagtcgacac
     1321 gtctcgagct ccctatcagt gatagagaag gtacgtctag aacgtctccc tatcagtgat
     1381 agagaagtcg acacgtctcg agctccctat cagtgataga gaaggtaccc cctatataag
     1441 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     1501 ccatagaaga caccgggacc gatccagcct ccgcggtggc ggccatcaca agtttgtaca
     1561 aaaaagcagg ctggcgccgg aaccaattca gtcgactgga tccggtaccg aattcaccat
     1621 gcccctcaac gtgaacttca ccaacaggaa ctatgacctc gactacgact ccgtacagcc
     1681 ctatttcatc tgcgacgagg aagagaattt ctatcaccag caacagcaga gcgagctgca
     1741 gccgcccgcg cccagtgagg atatctggaa gaaattcgag ctgcttccca ccccgcccct
     1801 gtccccgagc cgccgctccg ggctctgctc tccatcctat gttgcggtcg ctacgtcctt
     1861 ctccccaagg gaagacgatg acggcggcgg tggcaacttc tccaccgccg atcagctgga
     1921 gatgatgacc gagttacttg gaggagacat ggtgaaccag agcttcatct gcgatcctga
     1981 cgacgagacc ttcatcaaga acatcatcat ccaggactgt atgtggagcg gtttctcagc
     2041 cgctgccaag ctggtctcgg agaagctggc ctcctaccag gctgcgcgca aagacagcac
     2101 cagcctgagc cccgcccgcg ggcacagcgt ctgctccacc tccagcctgt acctgcagga
     2161 cctcaccgcc gccgcgtccg agtgcattga cccctcagtg gtctttccct acccgctcaa
     2221 cgacagcagc tcgcccaaat cctgtacctc gtccgattcc acggccttct ctccttcctc
     2281 ggactcgctg ctgtcctccg agtcctcccc acgggccagc cctgagcccc tagtgctgca
     2341 tgaggagaca ccgcccacca ccagcagcga ctctgaagaa gagcaagaag atgaggaaga
     2401 aattgatgtg gtgtctgtgg agaagaggca aacccctgcc aagaggtcgg agtcgggctc
     2461 atctccatcc cgaggccaca gcaaacctcc gcacagccca ctggtcctca agaggtgcca
     2521 cgtctccact caccagcaca actacgccgc acccccctcc acaaggaagg actatccagc
     2581 tgccaagagg gccaagttgg acagtggcag ggtcctgaag cagatcagca acaaccgcaa
     2641 gtgctccagc cccaggtcct cagacacgga ggaaaacgac aagaggcgga cacacaacgt
     2701 cttggaacgt cagaggagga acgagctgaa gcgcagcttt tttgccctgc gtgaccagat
     2761 ccctgaattg gaaaacaacg aaaaggcccc caaggtagtg atcctcaaaa aagccaccgc
     2821 ctacatcctg tccattcaag cagacgagca caagctcacc tctgaaaagg acttattgag
     2881 gaaacgacga gaacagttga aacacaaact cgaacagctt cgaaactctg gtgcaggttc
     2941 tggcgtgaaa cagactttga attttgacct tctcaagttg gcgggagacg tggagtccaa
     3001 cccagggccc atgaggcagc cacctggcga gtctgacatg gctgtcagcg acgctctgct
     3061 cccgtccttc tccacgttcg cgtccggccc ggcgggaagg gagaagacac tgcgtccagc
     3121 aggtgccccg actaaccgtt ggcgtgagga actctctcac atgaagcgac ttcccccact
     3181 tcccggccgc ccctacgacc tggcggcgac ggtggccaca gacctggaga gtggcggagc
     3241 tggtgcagct tgcagcagta acaacccggc cctcctagcc cggagggaga ccgaggagtt
     3301 caacgacctc ctggacctag actttatcct ttccaactcg ctaacccacc aggaatcggt
     3361 ggccgccacc gtgaccacct cggcgtcagc ttcatcctcg tcttccccag cgagcagcgg
     3421 ccctgccagc gcgccctcca cctgcagctt cagctatccg atccgggccg ggggtgaccc
     3481 gggcgtggct gccagcaaca caggtggagg gctcctctac agccgagaat ctgcgccacc
     3541 tcccacggcc cccttcaacc tggcggacat caatgacgtg agcccctcgg gcggcttcgt
     3601 ggctgagctc ctgcggccgg agttggaccc agtatacatt ccgccacagc agcctcagcc
     3661 gccaggtggc gggctgatgg gcaagtttgt gctgaaggcg tctctgacca cccctggcag
     3721 cgagtacagc agcccttcgg tcatcagtgt tagcaaagga agcccagacg gcagccaccc
     3781 cgtggtagtg gcgccctaca gcggtggccc gccgcgcatg tgccccaaga ttaagcaaga
     3841 ggcggtcccg tcctgcacgg tcagccggtc cctagaggcc catttgagcg ctggacccca
     3901 gctcagcaac ggccaccggc ccaacacaca cgacttcccc ctggggcggc agctccccac
     3961 caggactacc cctacactga gtcccgagga actgctgaac agcagggact gtcaccctgg
     4021 cctgcctctt cccccaggat tccatcccca tccggggccc aactaccctc ctttcctgcc
     4081 agaccagatg cagtcacaag tcccctctct ccattatcaa gagctcatgc caccgggttc
     4141 ctgcctgcca gaggagccca agccaaagag gggaagaagg tcgtggcccc ggaaaagaac
     4201 agccacccac acttgtgact atgcaggctg tggcaaaacc tataccaaga gttctcatct
     4261 caaggcacac ctgcgaactc acacaggcga gaaaccttac cactgtgact gggacggctg
     4321 tgggtggaaa ttcgcccgct ccgatgaact gaccaggcac taccgcaaac acacagggca
     4381 ccggcccttt cagtgccaga agtgcgacag ggccttttcc aggtcggacc accttgcctt
     4441 acacatgaag aggcactttg gctccggaga gggcagagga agtctgctaa catgcggtga
     4501 cgtcgaggag aatcctggcc cactcgagat ggctggacac ctggcttcag acttcgcctt
     4561 ctcaccccca ccaggtgggg gtgatgggtc agcagggctg gagccgggct gggtggattc
     4621 tcgaacctgg ctaagcttcc aagggcctcc aggtgggcct ggaatcggac caggctcaga
     4681 ggtattgggg atctccccat gtccgcccgc atacgagttc tgcggaggga tggcatactg
     4741 tggacctcag gttggactgg gcctagtccc ccaagttggc gtggagactt tgcagcctga
     4801 gggccaggca ggagcacgag tggaaagcaa ctcagaggga acctcctctg agccctgtgc
     4861 cgaccgcccc aatgccgtga agttggagaa ggtggaacca actcccgagg agtcccagga
     4921 catgaaagcc ctgcagaagg agctagaaca gtttgccaag ctgctgaagc agaagaggat
     4981 caccttgggg tacacccagg ccgacgtggg gctcaccctg ggcgttctct ttggaaaggt
     5041 gttcagccag accaccatct gtcgcttcga ggccttgcag ctcagcctta agaacatgtg
     5101 taagctgcgg cccctgctgg agaagtgggt ggaggaagcc gacaacaatg agaaccttca
     5161 ggagatatgc aaatcggaga ccctggtgca ggcccggaag agaaagcgaa ctagcattga
     5221 gaaccgtgtg aggtggagtc tggagaccat gtttctgaag tgcccgaagc cctccctaca
     5281 gcagatcact cacatcgcca atcagcttgg gctagagaag gatgtggttc gagtatggtt
     5341 ctgtaaccgg cgccagaagg gcaaaagatc aagtattgag tattcccaac gagaagagta
     5401 tgaggctaca gggacacctt tcccaggggg ggctgtatcc tttcctctgc ccccaggtcc
     5461 ccactttggc accccaggct atggaagccc ccacttcacc acactctact cagtcccttt
     5521 tcctgagggc gaggcctttc cctctgttcc cgtcactgct ctgggctctc ccatgcattc
     5581 aaacgggtcg ggtcaatgta ctaactacgc tttgttgaaa ctcgctggcg atgttgaaag
     5641 taaccccggt cctatgtata acatgatgga gacggagctg aagccgccgg gcccgcagca
     5701 agcttcgggg ggcggcggcg gaggaggcaa cgccacggcg gcggcgaccg gcggcaacca
     5761 gaagaacagc ccggaccgcg tcaagaggcc catgaacgcc ttcatggtat ggtcccgggg
     5821 gcagcggcgt aagatggccc aggagaaccc caagatgcac aactcggaga tcagcaagcg
     5881 cctgggcgcg gagtggaaac ttttgtccga gaccgagaag cggccgttca tcgacgaggc
     5941 caagcggctg cgcgctctgc acatgaagga gcacccggat tataaatacc ggccgcggcg
     6001 gaaaaccaag acgctcatga agaaggataa gtacacgctt cccggaggct tgctggcccc
     6061 cggcgggaac agcatggcga gcggggttgg ggtgggcgcc ggcctgggtg cgggcgtgaa
     6121 ccagcgcatg gacagctacg cgcacatgaa cggctggagc aacggcagct acagcatgat
     6181 gcaggagcag ctgggctacc cgcagcaccc gggcctcaac gctcacggcg cggcacagat
     6241 gcaaccgatg caccgctacg acgtcagcgc cctgcagtac aactccatga ccagctcgca
     6301 gacctacatg aacggctcgc ccacctacag catgtcctac tcgcagcagg gcacccccgg
     6361 tatggcgctg ggctccatgg gctctgtggt caagtccgag gccagctcca gcccccccgt
     6421 ggttacctct tcctcccact ccagggcgcc ctgccaggcc ggggacctcc gggacatgat
     6481 cagcatgtac ctccccggcg ccgaggtgcc ggagcccgct gcgcccagta gactgcacat
     6541 ggcccagcac taccagagcg gcccggtgcc cggcacggcc attaacggca cactgcccct
     6601 gtcgcacatg tgagaattcg cggccgcact cgagatatct agacccagct ttcttgtaca
     6661 aagtggtgat ctagctaaaa cgcggcctcg aatcgaattc cgcccccccc cctctccctc
     6721 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
     6781 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
     6841 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
     6901 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
     6961 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
     7021 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
     7081 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
     7141 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac
     7201 atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt
     7261 cctttgaaaa acacgatgat aatatggcca caaccatggt gagcaagggc gaggaggata
     7321 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     7381 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     7441 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     7501 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     7561 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     7621 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     7681 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     7741 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     7801 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     7861 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     7921 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     7981 gcggcatgga cgagctgtac aagtaaagcg gccgcgatct ttttccctct gccaaaaatt
     8041 atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt
     8101 tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg
     8161 caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgccatat
     8221 gctggctgcc atgaacaaag gtggctataa agaggtcatc agtatatgaa acagccccct
     8281 gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt
     8341 tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc
     8401 agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct cttatgaaga
     8461 tccctcgacc tgcaggaatt cgatatcaag ctctagccaa caagctcgtc atcgctttgc
     8521 agaagagcag agaggatatg ctcatcgtct aaagaactac ccattttatt atatattagt
     8581 cacgatatct ataacaagaa aatatatata taataagtta tcacgtaagt agaacatgaa
     8641 ataacaatat aattatcgta tgagttaaat cttaaaagtc acgtaaaaga taatcatgcg
     8701 tcattttgac tcacgcggtc gttatagttc aaaatcagtg acacttaccg cattgacaag
     8761 cacgcctcac gggagctcca agcggcgact gagatgtcct aaatgcacag cgacggattc
     8821 gcgctattta gaaagagaga gcaatatttc aagaatgcat gcgtcaattt tacgcagact
     8881 atctttctag ggttaaaaaa gatttgcgct ttactcgacc taaactttaa acacgtcata
     8941 gaatcttcgt ttgacaaaaa ccacattgtg gccaagctgt gtgacgcgac gcgcgctaaa
     9001 gaatggcaaa ccaagtcgcg cgaggtaccc agcttttgtt ccctttagtg agggttaatt
     9061 ccgagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     9121 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     9181 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     9241 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     9301 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     9361 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     9421 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     9481 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     9541 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     9601 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     9661 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     9721 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     9781 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     9841 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     9901 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     9961 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
    10021 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
    10081 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
    10141 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
    10201 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
    10261 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
    10321 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
    10381 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
    10441 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
    10501 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
    10561 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
    10621 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
    10681 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    10741 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    10801 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
    10861 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    10921 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    10981 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    11041 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    11101 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    11161 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    11221 aaagtgccac ctg
//
