LOCUS       Exported                 973 bp ds-DNA     linear   SYN 19-MAY-2021
DEFINITION  Used to construct AAV-based targeting vectors that carry an 
            IVS-2Aneo cassette for promoter-trapping.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-2Aneo
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Karnan S, Ota A, Konishi Y, Wahiduzzaman M, Hosokawa Y, Konishi H
  TITLE     Improved methods of AAV-mediated gene targeting for human cell lines
            using ribosome-skipping 2A peptide.
  JOURNAL   Nucleic Acids Res. 2015 Dec 10. pii: gkv1338.
  PUBMED    26657635
REFERENCE   2  (bases 1 to 973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    complement(33..66)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     polyA_signal    163..297
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(200..219)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     254..273
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(430..449)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 atacatatgc acagtggtac cttaaaattt ggataacttc gtataatgta tgctatacga
       61 agttattccc aactcatccc ggcctcgccg gggcccccta gaaaaaacct cccacacctc
      121 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
      181 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
      241 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcccc
      301 cgaaccccag agtcccgctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg
      361 cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag
      421 ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag
      481 ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca
      541 ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc atgcgcgcct tgagcctggc
      601 gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag
      661 accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg
      721 gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt
      781 ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag
      841 ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt
      901 ggccagccac gatagccgcg ctgcctcgtc ctgcagttca ttcagggcac cggacaggtc
      961 ggtcttgaca aaa
//
