LOCUS Exported 7336 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pGL2-NOS2Promoter-Luciferase SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7336) AUTHORS Lowenstein CJ, Alley EW, Raval P, Snowman AM, Snyder SH, Russell SW, Murphy WJ TITLE Macrophage nitric oxide synthase gene: two upstream regions mediate induction by interferon gamma and lipopolysaccharide. JOURNAL Proc Natl Acad Sci U S A. 1993 Oct 15. 90(20):9730-4. PUBMED 7692452 REFERENCE 2 (bases 1 to 7336) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7336 /organism="synthetic DNA construct" /mol_type="other DNA" polyA_signal 119..240 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" CDS 2079..3731 /codon_start=1 /gene="luc" /product="firefly luciferase" /label=luciferase /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL" primer_bind complement(2145..2163) /label=LucNrev /note="Firefly luciferase, reverse primer" intron 3972..4037 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 4167..4187 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" polyA_signal 4612..4746 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4888..4905) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(5059..5647) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(5139..5158) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5818..6678) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 6441..6460 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6679..6783) /gene="bla" /label=AmpR promoter rep_origin 6810..7265 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6897..6916) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7107..7128 /label=F1ori-F /note="F1 origin, forward primer" ORIGIN 1 cccaagctac catgataagt aagtaatatt aaggtacgtg gaggttttac ttgctttaaa 61 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa 121 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 181 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 241 tggtactgta actgagctaa cataacccgg gaggtaccga gctcttacgc ggactttgat 301 atgctgaaat ccataagctg tgtgtgtgtg caagtgtgca tgcgcatgtg tgcacatgag 361 tgtgcaggta tatgtaggag ctagaagaca atctcagctc ttgtttccca ggttacccag 421 catctctcac cagcctggaa cctgcctagt aggctaggct ggctggccag caaaccctag 481 gcatattcct gtctttacct ccccagaact tattgcaagt gtgtgtcacc acacccagca 541 ttttatcatt gacctattga ctggtgtgtg tgtgtgtgtg tgtgtttgtg tgtgtgtgtg 601 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtaaatt ccttatctca ccaacccatg 661 cccagctttt gaacttaggt ccttgtacat gcaaggcaag cactttacca actgagccat 721 ctccccagcc caattacttg atttgtaatt catttattca atcaacaatt tatttgttct 781 cccaactatt gaggccacac actttttggg tgacttagtc tgtgtacctc agacaagggc 841 aaaacacgag gctgagctga ctttggggac catgcgaaga tgagtggacc ctggcaggat 901 gtgctagggg gattttccct ctctctgttt gttccttttc ccctaacact gtcaatattt 961 cactttcata atggaaaatt ccatgccatg tgtgaatgct ttattggaag cattgtaaga 1021 aattataatt tattcgtttt tgtttgtttc tcagaacagg gtttttctgt gtagtgttcc 1081 tggcttatcc tagaacttac tctgtagacc aggctagccc aaactcaggg atcagccttt 1141 ctctgtctcc tgaatcccgg gattaaaggc ttatgccacc acacccaggt aggacattat 1201 aatcctatat ataagaagtc acccacacat acaaacacac acacaccaca cacacacaca 1261 cacacacaca cacacacaca gagagagaga gagagagaga gagggagaga gagagagaga 1321 gagagaatgc cactgagaaa aaaaataaaa aggcttcact cagcacagcc catccactat 1381 tctgcccaag ctgacttact actagtgggg aaatgctggt cagacggcat ctgtgcccac 1441 agcttgcctt ccatcctttc tagaaaacct cctgatgaat gtgtcctggg cgtgttggaa 1501 tattggcacc atctaacctc actgagagaa cagacagaaa gccagagagc tccgtgccca 1561 gaacaaaatc cctcagcagc tgcaagccag ggtatgtggt ttagctaaga aaagccagcc 1621 tccctcccta gtgagtccca gttttgaagt gactacgtgc tgcctagggg ccactgcctt 1681 ggacgggcga ccaggaagag atggccttgc atgaggatac accacagagt gatgtaatca 1741 agcacacaga ctaggagtgt ccatcatgaa tgagctaact tgcacaccca actggggact 1801 ctccctttgg gaacagttat gcaaaatagc tctgcagagc ctggaggggt ataaatacct 1861 gatggctgct gccagggtca caactttaca gggagttgaa gactgagact ctggccccac 1921 gggacacagt gtcactggtt tgaaacttct cagccacctt ggtgaaggga ctgagctgtt 1981 agagacactt ctgaggctcc tcacgcttgg gtcttgttca ctccacggag tagcctagtc 2041 gatctaagta agcttggcat tccggtactg ttggtaaaat ggaagacgcc aaaaacataa 2101 agaaaggccc ggcgccattc tatcctctag aggatggaac cgctggagag caactgcata 2161 aggctatgaa gagatacgcc ctggttcctg gaacaattgc ttttacagat gcacatatcg 2221 aggtgaacat cacgtacgcg gaatacttcg aaatgtccgt tcggttggca gaagctatga 2281 aacgatatgg gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat 2341 tctttatgcc ggtgttgggc gcgttattta tcggagttgc agttgcgccc gcgaacgaca 2401 tttataatga acgtgaattg ctcaacagta tgaacatttc gcagcctacc gtagtgtttg 2461 tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa aaaattacca ataatccaga 2521 aaattattat catggattct aaaacggatt accagggatt tcagtcgatg tacacgttcg 2581 tcacatctca tctacctccc ggttttaatg aatacgattt tgtaccagag tcctttgatc 2641 gtgacaaaac aattgcactg ataatgaatt cctctggatc tactgggtta cctaagggtg 2701 tggcccttcc gcatagaact gcctgcgtca gattctcgca tgccagagat cctatttttg 2761 gcaatcaaat cattccggat actgcgattt taagtgttgt tccattccat cacggttttg 2821 gaatgtttac tacactcgga tatttgatat gtggatttcg agtcgtctta atgtatagat 2881 ttgaagaaga gctgttttta cgatcccttc aggattacaa aattcaaagt gcgttgctag 2941 taccaaccct attttcattc ttcgccaaaa gcactctgat tgacaaatac gatttatcta 3001 atttacacga aattgcttct gggggcgcac ctctttcgaa agaagtcggg gaagcggttg 3061 caaaacgctt ccatcttcca gggatacgac aaggatatgg gctcactgag actacatcag 3121 ctattctgat tacacccgag ggggatgata aaccgggcgc ggtcggtaaa gttgttccat 3181 tttttgaagc gaaggttgtg gatctggata ccgggaaaac gctgggcgtt aatcagagag 3241 gcgaattatg tgtcagagga cctatgatta tgtccggtta tgtaaacaat ccggaagcga 3301 ccaacgcctt gattgacaag gatggatggc tacattctgg agacatagct tactgggacg 3361 aagacgaaca cttcttcata gttgaccgct tgaagtcttt aattaaatac aaaggatatc 3421 aggtggcccc cgctgaattg gaatcgatat tgttacaaca ccccaacatc ttcgacgcgg 3481 gcgtggcagg tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg 3541 agcacggaaa gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa 3601 ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg 3661 gaaaactcga cgcaagaaaa atcagagaga tcctcataaa ggccaagaag ggcggaaagt 3721 ccaaattgta aaatgtaact gtattcagcg atgacgaaat tcttagctat tgtaatactg 3781 cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt tattggtgcc cttaaacgcc 3841 tggttgctac gcctgaataa gtgataataa gcggatgaat ggcagaaatt cgccggatct 3901 ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct acagagattt 3961 aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa 4021 ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg 4081 cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat gatgaggcta 4141 ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa gaccccaagg 4201 actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat agaactcttg 4261 cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag aaaattatgg 4321 aaaaatattc tgtaaccttt ataagtaggc ataacagtta taatcataac atactgtttt 4381 ttcttactcc acacaggcat agagtgtctg ctattaataa ctatgctcaa aaattgtgta 4441 cctttagctt tttaatttgt aaaggggtta ataaggaata tttgatgtat agtgccttga 4501 ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc 4561 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt 4621 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 4681 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc 4741 tggatccgtc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg 4801 cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga 4861 caggtgccgg cagcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 4921 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 4981 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 5041 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 5101 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 5161 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 5221 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 5281 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 5341 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 5401 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 5461 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc 5521 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 5581 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 5641 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 5701 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 5761 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 5821 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 5881 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 5941 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 6001 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 6061 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 6121 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 6181 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 6241 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 6301 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 6361 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 6421 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 6481 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 6541 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 6601 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 6661 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 6721 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 6781 gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat taagcgcggc 6841 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc 6901 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa 6961 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact 7021 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt 7081 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa 7141 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt 7201 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac 7261 aatttgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc 7321 ttcgctatta cgccag //