LOCUS       Exported               10190 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Gateway-compatible zebrafish gfap promoter.
ACCESSION   .
VERSION     .
KEYWORDS    p5E-gfap
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10190)
  AUTHORS   Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne 
            P
  TITLE     A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate 
            Expression Constructs with Diverse Research Applications.
  JOURNAL   PLoS One. 2016 Aug 8;11(8):e0159277. doi: 
            10.1371/journal.pone.0159277. eCollection 2016.
  PUBMED    27500400
REFERENCE   2  (bases 1 to 10190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10190
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      268..295
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      387..473
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    593..688
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        690..708
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8164..8180)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8164..8180)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(8217..8235)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    8235..8359
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(8432..8450)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8455..8471)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8455..8471)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8573..8592)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             8584..9393
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(8651..8670)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      9540..10128
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10029..10048
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccgc gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttggt aatacgactc actatagggc gaattgggta
      721 ccgggccccc cctcgaggta aggactgagg tgatgcacag tgttgggtat gttactttca
      781 ataagttatt ctttgctaat tattagtcat ttgaattgta ttgaaattac atttttagtt
      841 actttgccta aaaaaaaact tgataagtaa tcgaattaca tacttgaagt ccacataaat
      901 ctcattcatt cattccttct cttttcgact tagtcccttt attaatcagg ggtttctaca
      961 gcggaacgaa cctcatatgt ttcaatttag tgactatttt ttataaatta ttgacaatgt
     1021 ttttgttgct tgttcaaact acttatttaa aatgagctga aactgcacaa ttattgatat
     1081 ttcattggaa cagcttaatt ttttttatgt ttaatccact taaataaaat tccaattata
     1141 taatattcca attataaagt tacttaatca atttgtatta ggacaatatg aatgaattgt
     1201 gtggaaccct gcatttttta cagtgaacct ttccttgttt cttaaacatg ggtgattttc
     1261 ggagaaataa aatttgacaa tatctgaaat gtaaaaagaa aatattcagt tactctgcct
     1321 ttctaaatgc atagtttata ttatcatcag agttgtaaag taactagtta caaatactca
     1381 aattactgta attgagtagt ttttttcagg aattgtaatt tactaagtag ctaaaaaatg
     1441 tgtactttta ctttcccttg agtacattat taatgctgta acggtacttt tactccacta
     1501 ctttccttca gcctgcactc actactttgt tttatcttgt ctatgaggat tagaaaaatc
     1561 aatcctgtga ttccaatcaa atcgcacatc caaagtcttt acaaaagcat tttgtttgga
     1621 tgtgaaaatc gggttgatgt cagaactcaa tgtcagccca acgtcaatgt ccaacctaaa
     1681 atcaacctaa tatcaacgtc taatgatgtt acagcttgac gttgtgtggg cgttaccatt
     1741 atttgtcaga cattggattt tagttgccat acctgataaa taaatgtcag tatttgatgt
     1801 ccgtatgatc ttggatttag atgttggctc gacattggat tttggtcacc tgacataaca
     1861 acctaaatct atcctaataa taacgtctta tgacgttgtg tgccaaccat ttttgtttat
     1921 taaaaattta tataaacatt tacaataata tattatccat gtgatttaat agaaatgtta
     1981 tttgtttttt ttctccccca tatttctgaa tgacttaaag cttttattat caggataagg
     2041 ttgtggtttt tattttttgc ttgctaactt caattgctat tgcatttaca tttatgcaaa
     2101 tggggagacg cggtgggtca gttggcgttt ctgtgtggag tttgcatgtt ctccccgtgt
     2161 tcgcgtgggt tttctctggg tgctctggtt tcccctaaag acatgtagta taggtgaatt
     2221 gggtaagcta aattgtccgt agtgtgtatg tgtgaatgta tggatgtttt cctgtgatgg
     2281 gttgcagctg aaagggcatc cgctgtgtaa aacatatgct ggataagttg gcaattcatt
     2341 tcgctgtggt aaccccagat taatatcgga actacgccaa aaagaaaatg aatgaatgag
     2401 tgaattaatg caaatggctc atccttccac ctaattccag tgccttgcat tgcattcaag
     2461 ctctacaatt aatcagttcc tttattttca ctttccaaac tgaatccaac aactcatgag
     2521 gaccccaggg tgccttgatt taccagtgtc atgatgaaca caatctaccc cactttaacc
     2581 cactcgtcaa cataaatact cccttaaagg gtgtacttgt gtctgagtag aagtgtctgc
     2641 tgggttggag gtgtcgcaca ttccaggaca cgtcaagaag aacgctaaga gcaacaggag
     2701 aacaatgtca caaaaaaggt caaagaaacg ccatccatta gccagcttta taaacagctg
     2761 tctcgtggct aaagaagttc tcaagaataa ccaaccttgc atttttccac attagtttta
     2821 caccgcagca aagtcaccat gtggatttat agcatccaaa ccccattttc aatgagaggc
     2881 ttcacatttc tcgctctcat tcaatcccac actgcctctc ttttcagact aaatcctgat
     2941 aggatgagtg gtatgggggc ggggaggggg cacaatacct cagtccagct tccacaaaga
     3001 ctcaacacac ccatgcagat gtcctgttgt tggggaagtg catttgtaac caaatttcaa
     3061 actttcaact ttcatttgaa gcagtggaga ctgctccatt cctattcaag atcaatttat
     3121 tgaaatatga caacatttag aataactttg ttatatgtta ttatgtttta taatgcaatt
     3181 ttgtttctgt gacatacatt tttttattta gtgcagtctt cagagccaca ggaaccttga
     3241 gaatcattct aattatgatt tgctactcga aaaacatttt tcatcatcaa taataagaaa
     3301 ttaaaacggt atacatatag atgtttagta taacagttct tagtagttta aaagaacatt
     3361 tgtaacaaca ttccaacctt ttaataactt tagccttgct tacatttcta aaggagagcc
     3421 ttccagctgt ttttctgaac ccagtgatga atgtaaagtt cttaaaacca agttctaaga
     3481 atgttttaat gattacattt aacccaattt caagccaaat ttcctatacc acgagtaaaa
     3541 tatagttata attaggatag atctttggta aaataagata caattttaag atttgaattt
     3601 aaaagtagct aaattgtttt tttatgacag aacttttaag tttatagaat attaatacag
     3661 gacaaaactt gtaaatgtat ttaatttcct atgaatattt gataaaatat aaatgacatc
     3721 tatatgtaaa ttcatgaacc atgactctta tgaattatca gtgcacaaat tatgtttgat
     3781 gcacacttaa ggtcaaaagt ttagggtcag tttatttttt tttttatttt taattttatt
     3841 taataattta aataaaatta ttctgttcat caaggcacat ttattaaatg taacaataaa
     3901 ttgttaaata cttattaaat atttattttt tacttattgt atgcagtttc aattgaagtc
     3961 attattgctt ttattactat taccattaat aaaaataaca ttaataataa taataataat
     4021 aataataatt gatattacag tgatttctga aggatcacgt gactgaagac tggagtaatg
     4081 aagctgaaaa ctcatcattt aaatcactgg agtaaatgat tcaattaaat gatcatttaa
     4141 tgatcattct tttgaacagt tattttatag tgcaataaca tttcacagtt ttaccatttg
     4201 ttttgtattt ttgattcaat aaatgcagcc ttggtgagca ggagaagctt aatttaaaac
     4261 atttaaaact cctactgacc ccaaactttt gaccggtagt gtacatgtat tactgtatgt
     4321 tcaaactata tgcttttatt cataagtgtt tctattgctg tacatttgac tgtctgcatc
     4381 aggtgaagtg tatgaaagag tgtgtgttta ctccaccccg tgggaaagag cagtagctgc
     4441 aatgtgtgtg tgtgagtagt tagtgatgca tgagtgatcc actgaaccct gtccagcccc
     4501 ctgctgcagc tctcaacaca tcatgatagg tcacctctac tgggaggctt tcagcacagc
     4561 tctcacaact gatttatcag agagtactct gaaatggaga aattactttc atattaaaga
     4621 gcacacacac acacacacac acacacacac acacacacac acacacacac acacacacac
     4681 acacacctag ttcaccttat taacgtggtt aagcctttaa atgtcacttt aagctgtata
     4741 gaagtgtctt gaaaaatatc tattcaaata ttatttactg tcatcaagga aaagataaaa
     4801 taaatcagtt attagaaata agtttttaaa actattatac ttagaaatgt gctgaaacaa
     4861 tcttcctaga acagaacaga aattggggaa aaaataaaca aaaataaata aatctatcta
     4921 tctatagtgt gtataatgtg tgtagtgagt atgtttaaaa taggtcaact aatatgttca
     4981 taataaaaat acagcatcaa tggtttgggt gtttttagat ccaaaggtct taaacgtggt
     5041 tattgctggt tgttcagttt tttctaggct ctaagaaaaa tcattttaga aactgtaact
     5101 gtttccttat ccatctgaat tccacctttg ggatgtagtg gaacgggaga tcaactgcat
     5161 gatgctatca agtcaatatg gatcaaaatc tcaggaatat ttccaggacc ttattgaatc
     5221 tatgccatga aggattaagg cagttctgaa aggggaaatg cggtttaacc cggtaatagc
     5281 aaggtgtacc taataaagtg gccggtgagt gtatatattg ttattgtttc accttcagaa
     5341 atgacatatc tagacaatag tttccttttt cttttttaat tattgtactt ctgtaagatt
     5401 cttcaatgtt caatggtttt cttatgttaa aggcgcagag ctgtagtttt agtgaagtaa
     5461 cagcattccg ttgaccaaag ctgacagatg aaggagagtc agttctgacc ttgcagttgt
     5521 cagtcacaat taagcctggt ttggttacaa gttaaacgta gacctgcttg tggtcaggat
     5581 catcctttaa aagaactaga tttgttttta acaatttgta accggtgtgc tctgaagtgt
     5641 cttaaataaa gcatatagga agcaaagcag tgaaaaatgt tccttataat ctacgagcat
     5701 tagcttaaat gtataaacaa aataatatta attgtgtgtt cagatcaaat ataggtattg
     5761 ttacacctaa aataaaccca ttgctttaaa aaataattaa acacagtgta caatattagc
     5821 atacttcctc tgacatctct aagtaaaaca agagctttca cgatttactc actgacccat
     5881 cttacagcac ttctctgtga atttaggcca tcacaaatgc tgtgcccagt tctctgactg
     5941 ggtttgacag tcgcccatct gtgtccagca gtctgatgag ctcatcctct gtaaatctgg
     6001 ctggctgccg tgttgttttg tgtctgtccg tgagtctctc tggccttcat acactcctct
     6061 gctacttcat agactgctga tgagtgcaga ggccaaacca ttagtgcttt tctttttttt
     6121 tagacctcag taatgcacaa caatgcaaaa caagtagatt gagccttttg ttctttggac
     6181 tgagcatttg gttagaatag cttgaaggga tagttagaag atattttgaa gggaaactaa
     6241 aaacctgtta ccatttgaca tccaaagaag gaaaactaat atgattgagt ggttacagat
     6301 ttccaacact tttcaacata tattattttg tgttccacta aagaaacagt caaatattat
     6361 actgtcaacc aaagtactgc acttgactca aaaaagctct gcagcaagtt gcctcttatt
     6421 ctaggtcgag tcaacctttt tttctctctc tctgtcggtt cattcaaggt tcagtaaatg
     6481 atgacagaat tttcagcttt tattttgggt gaactaaccc tttatatatt tgttgtaagt
     6541 ttatttttct gtatattata aatatattag caacataatt ctaagctaca cttgtaccca
     6601 atcaagggtc acactttatt ttgatggtat gtttgtttga atttaagtta cattgcatct
     6661 acatgtcaac taattctcat tagattataa gaagactgtt aggttggggt tagtgtaagt
     6721 tgacatgtac ttgcaaagtt tctcagagtc agttaaatgt ctgttttgta gcagtattaa
     6781 cagatattaa gcacactgta tattaatact caaatgaacc gtcaaaataa agtgttaccg
     6841 taaaaattat ttccacatct caaggtagct ttatgaggaa agttttcttt aagattagtt
     6901 tcttcgaaca aacttgcatg ttaaatatgt gctcagatat ggggcacaca gacaaaatgt
     6961 ttattggacg aataacccct ttaacagtta accagcattt tttaaaggga tagtcagaag
     7021 atattttgaa ggaaactaaa aacctgtaac cattcgacaa tcgaagaagg taaaagaaat
     7081 atgatagagt agttacagtt tttcaacact ctttataata tattgtttag tattcaactg
     7141 aagaaatagt caaacacact gtaaaaaaag ttggtttaaa ttgactcaac tttcaactca
     7201 agtatagtac ttgacttgat ttaagttgag ttaactagac taaactaaaa gcactaaaag
     7261 cactgcggca agtcgccttt caattttagg ttgagtaaat tattattatt tttttttaca
     7321 gttcaggttc agtaaatgat aacataattt tcagttttgg gtgaactatc cctttatata
     7381 tttgttgtaa gttaattttt ctgtaaatta taaattaatt agcaaaaaag ctgcacttct
     7441 tcaccattaa ttaattaata catctcaaag tagctttata aggaaagttt tctttaatta
     7501 tgtttctttg catgttaaaa ttgtgctcag atatggagca cacaagcaaa atgtttattg
     7561 gatgaataac ccctttaaca tttaaccagc atgagttcaa gtaaacaaat caattcctat
     7621 gatttttctg ctaaaccgat ataaatcagt gctgctgaag agcatgtgtg tgatgttctc
     7681 agatcagcac aaaagaggga gcgccagttt cttttaataa tgaagaatta cgtaatcacc
     7741 tcctgtgttc cctgaaacca ttaaactccc agacagaaca aaactgtgac tgagaaagca
     7801 gagcaactcc ggagtaactc agaagatctg caaaccaact ctttagcatg aggtaagaga
     7861 gaattaaagt ccaatatcag tgctgatgcc atctcagtct ctctatttct gtgttccagc
     7921 agggtatgaa taggattttc tctgactcac tgtgaaaaga ggcagtcagg agactggctg
     7981 gacaagcaca aacaattgcg ggctcacaaa caccccctcg gcctttgccc tccccatcag
     8041 accccccctc tctctttcac cccccacatc aactctataa aaacccagag ttcacccgca
     8101 ctcgatctca ttctcctcca ccatggagtc ccagcgttcc tgtcgacggt accgcgggcc
     8161 cgggatccac tagttctaga gcggccgcca ccgcggtgga gctccagctt ttgttccctt
     8221 tagtgagggt taattcaagt ttgtacaaaa aagttgaacg agaaacgtaa aatgatataa
     8281 atatcaatat attaaattag attttgcata aaaaacagac tacataatac tgtaaaacac
     8341 aacatatgca gtcactatga accaactact tagatggtat tagtgacctg tagaattcga
     8401 gctctagagc tgcagggcgg ccgcgatatc ccctatagtg agtcgtatta catggtcata
     8461 gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat
     8521 aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt
     8581 gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct
     8641 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat
     8701 cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
     8761 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
     8821 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
     8881 cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
     8941 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
     9001 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt
     9061 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa
     9121 atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt
     9181 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga
     9241 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct
     9301 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg
     9361 cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac
     9421 tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa atcccttaac
     9481 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     9541 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     9601 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     9661 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     9721 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     9781 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     9841 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     9901 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg
     9961 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
    10021 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
    10081 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
    10141 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
//
