LOCUS       Exported                5760 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Gateway-compatible zebrafish mnx1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    p5E-mnx1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5760)
  AUTHORS   Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne 
            P
  TITLE     A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate 
            Expression Constructs with Diverse Research Applications.
  JOURNAL   PLoS One. 2016 Aug 8;11(8):e0159277. doi: 
            10.1371/journal.pone.0159277. eCollection 2016.
  PUBMED    27500400
REFERENCE   2  (bases 1 to 5760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5760
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      268..295
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      387..473
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    593..688
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    3805..3929
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4001..4020)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4002..4020)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4025..4041)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4025..4041)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4143..4162)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             4154..4963
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4221..4240)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      5110..5698
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5599..5618
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccgc gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttggg ccggccaatt catttaaatt agcctggcat
      721 ctggacatcg tcaatcacgg gctcgtgaca ccgagagggg actcgtgggt catctcgttt
      781 aaagcgcatt ttaaattaca ccagccactg aattgtgtgt attcatcaca ttagtgtgta
      841 ccagtgtgtt cggttaagta acacctggtt ttcgggctgg ttcatcagtt tgtttggtcc
      901 agtgaatcac ttaagagata atcaggctta tcaaagcaaa ctgaggaaaa cgctttcgac
      961 tacagaagga ctttgtatta tgcaatgttt tcaaaaaaat atcttcatat tcagtgtggg
     1021 agataataag aaaggaaaaa gcatgaagca ctgaatcggt gtcctttaat gacttttata
     1081 gttgggaaca attagccgtt ctccacaaat taaattgata aaaagattac gagacccaaa
     1141 tgcgtttaaa agctgtggta cgcacggccc atttgtgcaa ttatgatcga aaatatttat
     1201 tgcaataaaa gagacagtgc aggtcccgtt aaattggctt ttcataatga acacatttaa
     1261 catggataat taatatataa actatttcga tttgtcacga tgatatgtgt tgcacttaat
     1321 tgtgaaagta attaccgagc gaatgaacat ctttgaggag agtccctggt ttcaggccct
     1381 cgtatcatcg ggtgcaataa taattttact ctctcctgat gtgatgaaaa agagccataa
     1441 atctctcaga tcgctgcagg tccacggctt catatttcac cgctgcttta atagaaatat
     1501 tcatgcgatc ccttaatgaa attaaaactg agaagcggac aaagcggccc taggctatgt
     1561 gcagcgtgta aagacacaag agcatttgat tgggaaaatg ttgcatcctt caaatatgag
     1621 gatgttgttg cactgtaaac cacgtaaaac attttttttc ttacagtcca gtattggcca
     1681 cacttgataa ttgttacatt tgttatcaat tacacgcgag gctcattgaa tgtcatttca
     1741 gaattggtca gtgcatgcgt cattacggca cgctgttatt ctcatccttt cggccggtga
     1801 cttgttgatt tccctcgagc aaataaacgc atgcgaccag tctctcgaga gtggttagct
     1861 gatgaattga ggaaaactaa tccgctttat tggtagacag gttaagggca acagagtgtc
     1921 aataattctc tccctgacct ctttctccat tagcttaaag aggcttataa gaggaacaga
     1981 ccccacaaca tgacaccgat cctccacatc accatctcac atgtagccaa cctaggcttg
     2041 gaaaatcatt tcgatttata aagctcaaga tccaacattt gccaaaaatt catgaaaaca
     2101 gggcacaaat agggcatcat tttctcacaa agtattatat aggcctaatt agatagatag
     2161 atagatagat agatagatag atagatagat agatagatag atagatagat agatagatag
     2221 atagatagat agatagatag atagatagat agatagatag atagatagat agatagatag
     2281 atagatagat agatagatag atagatagac agatagatag atagaaaaaa aacaagattt
     2341 tgcttgtgat tcattatgca taataatttg cctatgttta ttaataaaca tcgtgttagg
     2401 attataaatg tgttgtgatg aatgtgatta aatgtttaaa tatttactga cagttgttag
     2461 cctatattta cagatgtaaa aaatctacgt ctaattagta tgtgtttctt aaatctataa
     2521 taaggaaagg cttattaagt ttatctgtac aatttagaac aattttacga atttatcctt
     2581 tagagaaaaa aaatgtaata acaccatatt taaatatcct gttgagaaat tattaaatca
     2641 aatttttaca ttctaagagc aaggttaaat attgagtgaa aggttgaatt gtttttaaga
     2701 cagttgtttt atttttttaa gcatattgtg gtgggtgagt tttcaaagat ttacacattt
     2761 caaacaattt attaaaacaa tatttgttgc ataaaacact gatttggtca gttttaccac
     2821 tgactttaac ttatttattt aattaattat atttctattg ttcattgtta ttaaattttg
     2881 cctacctaat gctctctaac aaataaggaa taacttgaat atttcctctg cgcaaaatgt
     2941 tcatcgtaat gtaaaagatc cgtttgacat ttaaaatggt tctcttctca taaagatagt
     3001 aattctttta agaactgtcc tctagaaggt tctttaggga aacaaaaaat atacgtgtac
     3061 atcaatgtga aaatcaaata gtttttttta ttattgcagg ctgttcagat aaaaaataca
     3121 attgaataaa aaagtatgga cattaaatag caaaatgaca aatgcgtgta tagactatgt
     3181 tattttgtta attcatttga tgaaataaaa aaaaattata caaatgaaaa aatgtcatgc
     3241 ctctacatct atgcgaataa aaagcatggc tgtttttttt tttttttttt ttggtcaaac
     3301 aaactatttc gccaaaataa tattttcgag ttaacatgct ttatcaatct tcgtttatat
     3361 taagattata ggtcagtgtt taaataatga aatcttgata actttataaa tgtttcatgt
     3421 ttcaaagttg catgttaata attgccctta gactctacaa atctggaatt attcataaat
     3481 taagtagatg taaaatctat gtgtgcatgt tgtgtttccc acttaaaaag ctttcatttt
     3541 atgggagaac atacttatga caactcttga acttctacaa gcacaccgtg aacactggaa
     3601 tcactgtcca atcggaaatg cacaaggatg acgtgaatga ccaatccact ccctctcggg
     3661 cgcagttgac aagtggactt ctggcttgca caccttggcc aagatcataa ctttacgaag
     3721 aggagcggtg acactcctga cagcctttta aagttacgaa cgaggcgtta atctgtttgt
     3781 gaggtgggcc agggatcggc gcgcccaagt ttgtacaaaa aagttgaacg agaaacgtaa
     3841 aatgatataa atatcaatat attaaattag attttgcata aaaaacagac tacataatac
     3901 tgtaaaacac aacatatgca gtcactatga atcaactact tagatggtat tagtgacctg
     3961 tagaattcga gctctagagc tgcagggcgg ccgcgatatc ccctatagtg agtcgtatta
     4021 catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat
     4081 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa
     4141 tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa
     4201 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc
     4261 gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa
     4321 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt
     4381 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac
     4441 cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga
     4501 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa
     4561 ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa
     4621 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt
     4681 ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga
     4741 tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg
     4801 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga
     4861 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat
     4921 gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg
     4981 gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa
     5041 atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     5101 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     5161 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     5221 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     5281 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     5341 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     5401 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     5461 gcgaacgacc tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct
     5521 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     5581 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     5641 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     5701 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
//
