LOCUS Exported 757 bp ds-DNA linear SYN 08-SEP-2021 DEFINITION This plasmid encodes the kinase domain of KC1G1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. . ACCESSION . VERSION . KEYWORDS KC1G1_HUMAN_D0 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 757) AUTHORS Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD TITLE An open library of human kinase domain constructs for automated bacterial expression. JOURNAL Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081. PUBMED 30004690 REFERENCE 2 (bases 1 to 757) AUTHORS . TITLE Direct Submission JOURNAL Exported Sep 8, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..757 /organism="synthetic DNA construct" /mol_type="other DNA" RBS 42..64 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" CDS 84..113 /codon_start=1 /product="10xHis affinity tag" /label=10xHis /translation="HHHHHHHHHH" CDS 114..134 /codon_start=1 /product="tobacco etch virus (TEV) protease recognition and cleavage site" /label=TEV site /translation="ENLYFQS" ORIGIN 1 attaagcggc cgccctgcag gactcgagtt ctagaaataa ttttgtttaa ctttaagaag 61 gagatataca tatgaaatct tctcaccatc accatcacca tcaccatcac catgaaaacc 121 tgtacttcca atccaatgca agggttggca agaagatagg atgtgggaac ttcggagagc 181 tcagattagg taaaaatctc tacaccaatg aatatgtagc aatcaaactg gaaccaataa 241 aatcacgtgc tccacagctt catttagagt acagatttta taaacagctt ggcagtgcag 301 gtgaaggtct cccacaggtg tattactttg gaccatgtgg gaaatataat gccatggtgc 361 tggagctcct tggccctagc ttggaggact tgtttgacct ctgtgaccga acatttactt 421 tgaagacggt gttaatgata gccatccagc tgctttctcg aatggaatac gtgcactcaa 481 agaacctcat ttaccgagat gtcaagccag agaacttcct gattggtcga caaggcaata 541 agaaagagca tgttatacac attatagact ttggactggc caaggaatac attgaccccg 601 aaaccaaaaa acacatacct tatagggaac acaaaagttt aactggaact gcaagatata 661 tgtctatcaa cacgcatctt ggcaaagagc aaagccggag agatgatttg gaagccctag 721 gccatatgtt catgtatttc cttcgaggca gcctccc //