LOCUS       Exported               11381 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  piggyBac transposon for dox-inducible expression of the OKMS (Oct4, 
            Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette 
            (with mCherry).
ACCESSION   .
VERSION     .
KEYWORDS    PB-TAC-OKMS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11381)
  AUTHORS   Kim SI, Oceguera-Yanez F, Hirohata R, Linker S, Okita K, Yamada Y, 
            Yamamoto T, Yamanaka S, Woltjen K
  TITLE     KLF4 N-terminal variance modulates induced reprogramming to 
            pluripotency.
  JOURNAL   Stem Cell Reports. 2015 Apr 14;4(4):727-43. doi: 
            10.1016/j.stemcr.2015.02.004. Epub 2015 Mar 12.
  PUBMED    25772473
REFERENCE   2  (bases 1 to 11381)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11381
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     588..610
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     602..619
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   711..773
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    1145..1163
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1182..1200
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1219..1237
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1256..1274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1293..1311
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1330..1348
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1367..1385
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1404..1422
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1444..1468
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    1548..1572
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2725..2790
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             4231..4290
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             5686..5751
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     protein_bind    complement(6791..6815)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6857..7443
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7024..7041)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7251..7270
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7444..8154
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7589..7607)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7857..7876
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(8166..8185)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8231..8286
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8285..8304)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     repeat_region   9006..9040
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(9190..9208)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9229..9245)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9229..9245)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9242..9264)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9253..9269
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9277..9307)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9322..9343
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9460..9477)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9631..10219)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9711..9730)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10390..11250)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11013..11032
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11251..11355)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg cgccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcgg
      661 tattcacgac agcaggctga ataataaaaa aattagaaac tattatttaa ccctagaaag
      721 ataatcatat tgtgacgtac gttaaagata atcatgcgta aaattgacgc atgtgtttta
      781 tcggtctgta tatcgaggtt tatttattaa tttgaataga tattaagttt tattatattt
      841 acacttacat actaataata aattcaacaa acaatttatt tatgtttatt tatttattaa
      901 aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa aacttttaaa cattctctct
      961 tttacaaaaa taaacttatt ttgtacttta aaaacagtca tgttgtatta taaaataagt
     1021 aattagctta acctatacat aatagaaaca aattatactt attagtcagt cagaaacaac
     1081 tttggcacat atcaatatta tgctctcgtt aattaactcg agggatccaa gctggccgca
     1141 cgtctcccta tcagtgatag agaagtcgac acgtctcgag ctccctatca gtgatagaga
     1201 aggtacgtct agaacgtctc cctatcagtg atagagaagt cgacacgtct cgagctccct
     1261 atcagtgata gagaaggtac gtctagaacg tctccctatc agtgatagag aagtcgacac
     1321 gtctcgagct ccctatcagt gatagagaag gtacgtctag aacgtctccc tatcagtgat
     1381 agagaagtcg acacgtctcg agctccctat cagtgataga gaaggtaccc cctatataag
     1441 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     1501 ccatagaaga caccgggacc gatccagcct ccgcggtggc ggccatcaca agtttgtaca
     1561 aaaaagcagg ctggcgccgg aaccaattca gtcgactgga tccggtaccg aattcgccct
     1621 taaaaagcag gctccacctt ccccatggct ggacacctgg cttcagactt cgccttctca
     1681 cccccaccag gtgggggtga tgggtcagca gggctggagc cgggctgggt ggattctcga
     1741 acctggctaa gcttccaagg gcctccaggt gggcctggaa tcggaccagg ctcagaggta
     1801 ttggggatct ccccatgtcc gcccgcatac gagttctgcg gagggatggc atactgtgga
     1861 cctcaggttg gactgggcct agtcccccaa gttggcgtgg agactttgca gcctgagggc
     1921 caggcaggag cacgagtgga aagcaactca gagggaacct cctctgagcc ctgtgccgac
     1981 cgccccaatg ccgtgaagtt ggagaaggtg gaaccaactc ccgaggagtc ccaggacatg
     2041 aaagccctgc agaaggagct agaacagttt gccaagctgc tgaagcagaa gaggatcacc
     2101 ttggggtaca cccaggccga cgtggggctc accctgggcg ttctctttgg aaaggtgttc
     2161 agccagacca ccatctgtcg cttcgaggcc ttgcagctca gccttaagaa catgtgtaag
     2221 ctgcggcccc tgctggagaa gtgggtggag gaagccgaca acaatgagaa ccttcaggag
     2281 atatgcaaat cggagaccct ggtgcaggcc cggaagagaa agcgaactag cattgagaac
     2341 cgtgtgaggt ggagtctgga gaccatgttt ctgaagtgcc cgaagccctc cctacagcag
     2401 atcactcaca tcgccaatca gcttgggcta gagaaggatg tggttcgagt atggttctgt
     2461 aaccggcgcc agaagggcaa aagatcaagt attgagtatt cccaacgaga agagtatgag
     2521 gctacaggga cacctttccc agggggggct gtatcctttc ctctgccccc aggtccccac
     2581 tttggcaccc caggctatgg aagcccccac ttcaccacac tctactcagt cccttttcct
     2641 gagggcgagg cctttccctc tgttcccgtc actgctctgg gctctcccat gcattcaaac
     2701 ggggatctca aaattgtcgc tcctgtcaaa caaactctta actttgattt actcaaactg
     2761 gctggggatg tagaaagcaa tccaggtcca ctcatggctg tcagcgacgc tctgctcccg
     2821 tccttctcca cgttcgcgtc cggcccggcg ggaagggaga agacactgcg tccagcaggt
     2881 gccccgacta accgttggcg tgaggaactc tctcacatga agcgacttcc cccacttccc
     2941 ggccgcccct acgacctggc ggcgacggtg gccacagacc tggagagtgg cggagctggt
     3001 gcagcttgca gcagtaacaa cccggccctc ctagcccgga gggagaccga ggagttcaac
     3061 gacctcctgg acctagactt tatcctttcc aactcgctaa cccaccagga atcggtggcc
     3121 gccaccgtga ccacctcggc gtcagcttca tcctcgtctt ccccagcgag cagcggccct
     3181 gccagcgcgc cctccacctg cagcttcagc tatccgatcc gggccggggg tgacccgggc
     3241 gtggctgcca gcaacacagg tggagggctc ctctacagcc gagaatctgc gccacctccc
     3301 acggccccct tcaacctggc ggacatcaat gacgtgagcc cctcgggcgg cttcgtggct
     3361 gagctcctgc ggccggagtt ggacccagta tacattccgc cacagcagcc tcagccgcca
     3421 ggtggcgggc tgatgggcaa gtttgtgctg aaggcgtctc tgaccacccc tggcagcgag
     3481 tacagcagcc cttcggtcat cagtgttagc aaaggaagcc cagacggcag ccaccccgtg
     3541 gtagtggcgc cctacagcgg tggcccgccg cgcatgtgcc ccaagattaa gcaagaggcg
     3601 gtcccgtcct gcacggtcag ccggtcccta gaggcccatt tgagcgctgg accccagctc
     3661 agcaacggcc accggcccaa cacacacgac ttccccctgg ggcggcagct ccccaccagg
     3721 actaccccta cactgagtcc cgaggaactg ctgaacagca gggactgtca ccctggcctg
     3781 cctcttcccc caggattcca tccccatccg gggcccaact accctccttt cctgccagac
     3841 cagatgcagt cacaagtccc ctctctccat tatcaagagc tcatgccacc gggttcctgc
     3901 ctgccagagg agcccaagcc aaagagggga agaaggtcgt ggccccggaa aagaacagcc
     3961 acccacactt gtgactatgc aggctgtggc aaaacctata ccaagagttc tcatctcaag
     4021 gcacacctgc gaactcacac aggcgagaaa ccttaccact gtgactggga cggctgtggg
     4081 tggaaattcg cccgctccga tgaactgacc aggcactacc gcaaacacac agggcaccgg
     4141 ccctttcagt gccagaagtg cgacagggcc ttttccaggt cggaccacct tgccttacac
     4201 atgaagaggc actttctcga gggctcgggc cagtgtacta attatgctct cttgaaattg
     4261 gctggagatg ttgagagcaa cccaggtccc ggcgcgccac tggatttcct ttgggcgttg
     4321 gaaaccccgc agacagccac gacgatgccc ctcaacgtga acttcaccaa caggaactat
     4381 gacctcgact acgactccgt acagccctat ttcatctgcg acgaggaaga gaatttctat
     4441 caccagcaac agcagagcga gctgcagccg cccgcgccca gtgaggatat ctggaagaaa
     4501 ttcgagctgc ttcccacccc gcccctgtcc ccgagccgcc gctccgggct ctgctctcca
     4561 tcctatgttg cggtcgctac gtccttctcc ccaagggaag acgatgacgg cggcggtggc
     4621 aacttctcca ccgccgatca gctggagatg atgaccgagt tacttggagg agacatggtg
     4681 aaccagagct tcatctgcga tcctgacgac gagaccttca tcaagaacat catcatccag
     4741 gactgtatgt ggagcggttt ctcagccgct gccaagctgg tctcggagaa gctggcctcc
     4801 taccaggctg cgcgcaaaga cagcaccagc ctgagccccg cccgcgggca cagcgtctgc
     4861 tccacctcca gcctgtacct gcaggacctc accgccgccg cgtccgagtg cattgacccc
     4921 tcagtggtct ttccctaccc gctcaacgac agcagctcgc ccaaatcctg tacctcgtcc
     4981 gattccacgg ccttctctcc ttcctcggac tcgctgctgt cctccgagtc ctccccacgg
     5041 gccagccctg agcccctagt gctgcatgag gagacaccgc ccaccaccag cagcgactct
     5101 gaagaagagc aagaagatga ggaagaaatt gatgtggtgt ctgtggagaa gaggcaaacc
     5161 cctgccaaga ggtcggagtc gggctcatct ccatcccgag gccacagcaa acctccgcac
     5221 agcccactgg tcctcaagag gtgccacgtc tccactcacc agcacaacta cgccgcaccc
     5281 ccctccacaa ggaaggacta tccagctgcc aagagggcca agttggacag tggcagggtc
     5341 ctgaagcaga tcagcaacaa ccgcaagtgc tccagcccca ggtcctcaga cacggaggaa
     5401 aacgacaaga ggcggacaca caacgtcttg gaacgtcaga ggaggaacga gctgaagcgc
     5461 agcttttttg ccctgcgtga ccagatccct gaattggaaa acaacgaaaa ggcccccaag
     5521 gtagtgatcc tcaaaaaagc caccgcctac atcctgtcca ttcaagcaga cgagcacaag
     5581 ctcacctctg aaaaggactt attgaggaaa cgacgagaac agttgaaaca caaactcgaa
     5641 cagcttcgaa actctggtgc aggggatctc aaaattgtcg ctcctgtcaa acaaactctt
     5701 aactttgatt tactcaaact ggctggggat gtagaaagca atccaggtcc actcatgaac
     5761 aattcgccct tcaccatgta taacatgatg gagacggagc tgaagccgcc gggcccgcag
     5821 caagcttcgg ggggcggcgg cggaggaggc aacgccacgg cggcggcgac cggcggcaac
     5881 cagaagaaca gcccggaccg cgtcaagagg cccatgaacg ccttcatggt atggtcccgg
     5941 gggcagcggc gtaagatggc ccaggagaac cccaagatgc acaactcgga gatcagcaag
     6001 cgcctgggcg cggagtggaa acttttgtcc gagaccgaga agcggccgtt catcgacgag
     6061 gccaagcggc tgcgcgctct gcacatgaag gagcacccgg attataaata ccggccgcgg
     6121 cggaaaacca agacgctcat gaagaaggat aagtacacgc ttcccggagg cttgctggcc
     6181 cccggcggga acagcatggc gagcggggtt ggggtgggcg ccggcctggg tgcgggcgtg
     6241 aaccagcgca tggacagcta cgcgcacatg aacggctgga gcaacggcag ctacagcatg
     6301 atgcaggagc agctgggcta cccgcagcac ccgggcctca acgctcacgg cgcggcacag
     6361 atgcaaccga tgcaccgcta cgacgtcagc gccctgcagt acaactccat gaccagctcg
     6421 cagacctaca tgaacggctc gcccacctac agcatgtcct actcgcagca gggcaccccc
     6481 ggtatggcgc tgggctccat gggctctgtg gtcaagtccg aggccagctc cagccccccc
     6541 gtggttacct cttcctccca ctccagggcg ccctgccagg ccggggacct ccgggacatg
     6601 atcagcatgt acctccccgg cgccgaggtg ccggagcccg ctgcgcccag tagactgcac
     6661 atggcccagc actaccagag cggcccggtg cccggcacgg ccattaacgg cacactgccc
     6721 ctgtcgcaca tgtgagggct ggaagggcga attgcctgca ggaattcgcg gccgcactcg
     6781 agatatctag acccagcttt cttgtacaaa gtggtgatct agctaaaacg cggcctcgaa
     6841 tcgaattccg cccccccccc tctccctccc ccccccctaa cgttactggc cgaagccgct
     6901 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg
     6961 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt
     7021 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg
     7081 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac
     7141 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg
     7201 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct
     7261 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg
     7321 atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag
     7381 gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca
     7441 accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
     7501 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
     7561 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
     7621 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
     7681 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
     7741 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
     7801 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
     7861 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
     7921 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
     7981 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
     8041 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     8101 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaaagcggc
     8161 cgcgatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct tgagcatctg
     8221 acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt
     8281 ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa tgagtatttg
     8341 gtttagagtt tggcaacata tgccatatgc tggctgccat gaacaaaggt ggctataaag
     8401 aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt
     8461 gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct
     8521 aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt
     8581 catagctgtc cctcttctct tatgaagatc cctcgacctg caggaattcg atatcaagct
     8641 ctagccaaca agctcgtcat cgctttgcag aagagcagag aggatatgct catcgtctaa
     8701 agaactaccc attttattat atattagtca cgatatctat aacaagaaaa tatatatata
     8761 ataagttatc acgtaagtag aacatgaaat aacaatataa ttatcgtatg agttaaatct
     8821 taaaagtcac gtaaaagata atcatgcgtc attttgactc acgcggtcgt tatagttcaa
     8881 aatcagtgac acttaccgca ttgacaagca cgcctcacgg gagctccaag cggcgactga
     8941 gatgtcctaa atgcacagcg acggattcgc gctatttaga aagagagagc aatatttcaa
     9001 gaatgcatgc gtcaatttta cgcagactat ctttctaggg ttaaaaaaga tttgcgcttt
     9061 actcgaccta aactttaaac acgtcataga atcttcgttt gacaaaaacc acattgtggc
     9121 caagctgtgt gacgcgacgc gcgctaaaga atggcaaacc aagtcgcgcg aggtacccag
     9181 cttttgttcc ctttagtgag ggttaattcc gagcttggcg taatcatggt catagctgtt
     9241 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     9301 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     9361 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     9421 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     9481 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     9541 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     9601 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     9661 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     9721 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     9781 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     9841 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     9901 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     9961 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
    10021 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
    10081 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    10141 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    10201 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    10261 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    10321 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    10381 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    10441 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    10501 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
    10561 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    10621 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    10681 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    10741 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    10801 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    10861 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    10921 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    10981 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    11041 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    11101 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    11161 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    11221 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    11281 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    11341 aataggggtt ccgcgcacat ttccccgaaa agtgccacct g
//
