LOCUS Exported 5225 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS dsRed-cent2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5225) AUTHORS Tanaka T, Serneo FF, Higgins C, Gambello MJ, Wynshaw-Boris A, Gleeson JG TITLE Lis1 and doublecortin function with dynein to mediate coupling of the nucleus to the centrosome in neuronal migration. JOURNAL J Cell Biol. 2004 Jun 7. 165(5):709-21. PUBMED 15173193 REFERENCE 2 (bases 1 to 5225) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5225 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 68..371 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 372..575 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 526..546 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 1213..1222 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1219..1899 /codon_start=1 /product="wild-type DsRed" /label=DsRed1 /note="mammalian codon-optimized" /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH HLFL" primer_bind complement(1402..1422) /label=DsRed1-N /note="DsRed1, reverse primer" primer_bind 1620..1639 /label=mCherry-F /note="mCherry, forward primer" primer_bind 1814..1837 /label=DsRed1-C /note="DsRed1, forward primer" polyA_signal 2020..2141 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2057..2076) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 2111..2130 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(2148..2603) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2285..2306) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2497..2516 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2630..2734 /gene="bla" /label=AmpR promoter promoter 2736..3093 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2759..2779) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 2944..3079 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3006..3025 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3128..3922 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3182..3201) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3792..3811 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(4110..4129) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 4154..4201 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4530..5118 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5019..5038 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg 61 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc 121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt 181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc 241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 541 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 601 agcgctaccg gactcagatc tcgagctcaa gcttcgaatt ctatggcctc ctccaacttt 661 aagaaggcaa acatggcatc aagttctcag cgaaaaagaa tgagccctaa gcctgagctt 721 actgaagagc aaaagcagga gatccgggaa gcttttgatc ttttcgatgc ggatggaact 781 ggcaccatag atgttaaaga actgaaggtg gcaatgaggg ccctgggctt tgaacccaag 841 aaagaagaaa ttaagaaaat gataagtgaa attgataagg aagggacagg aaaaatgaac 901 tttggtgact ttttaactgt gatgacccag aaaatgtctg agaaagatac taaagaagaa 961 atcctgaaag ctttcaagct ctttgatgat gatgaaactg ggaagatttc gttcaaaaat 1021 ctgaaacgcg tggccaagga gttgggtgag aacctgactg atgaggagct gcaggaaatg 1081 attgatgaag ctgatcgaga tggagatgga gaggtcagtg agcaagagtt cctgcgcatc 1141 atgaaaaaga ccagcctcta tcccgggatc attctgcagt cgacggtacc gcgggcccgg 1201 gatccaccgg tcgccaccat ggtgcgctcc tccaagaacg tcatcaagga gttcatgcgc 1261 ttcaaggtgc gcatggaggg caccgtgaac ggccacgagt tcgagatcga gggcgagggc 1321 gagggccgcc cctacgaggg ccacaacacc gtgaagctga aggtgaccaa gggcggcccc 1381 ctgcccttcg cctgggacat cctgtccccc cagttccagt acggctccaa ggtgtacgtg 1441 aagcaccccg ccgacatccc cgactacaag aagctgtcct tccccgaggg cttcaagtgg 1501 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg 1561 caggacggct gcttcatcta caaggtgaag ttcatcggcg tgaacttccc ctccgacggc 1621 cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc 1681 gacggcgtgc tgaagggcga gatccacaag gccctgaagc tgaaggacgg cggccactac 1741 ctggtggagt tcaagtccat ctacatggcc aagaagcccg tgcagctgcc cggctactac 1801 tacgtggact ccaagctgga catcacctcc cacaacgagg actacaccat cgtggagcag 1861 tacgagcgca ccgagggccg ccaccacctg ttcctgtagc ggccgcgact ctagatcata 1921 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc 1981 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat 2041 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg 2101 cattctagtt gtggtttgtc caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt 2161 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata 2221 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt 2281 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg 2341 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt 2401 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc 2461 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg 2521 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct 2581 taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc 2641 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg 2701 ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg 2761 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa 2821 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc 2881 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg 2941 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 3001 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga 3061 ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc 3121 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag 3181 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg 3241 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa 3301 tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc 3361 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc 3421 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga 3481 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa 3541 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct 3601 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat 3661 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt 3721 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta 3781 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga 3841 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg 3901 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg 3961 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc 4021 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag 4081 ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat accggaagga 4141 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt 4201 cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat 4261 tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa 4321 ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca 4381 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc 4441 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 4501 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 4561 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 4621 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt 4681 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 4741 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 4801 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 4861 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 4921 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 4981 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 5041 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 5101 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 5161 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt 5221 accgc //