LOCUS Exported 939 bp ds-DNA linear SYN 19-JUL-2021 DEFINITION Updated TALEN vector; compatible with the Golden Gate TALEN assembly protocol. More stable backbone with reduced recombination compared to pT3Ts-goldyTALEN due to the removal of a lacZ sequence. ACCESSION . VERSION . KEYWORDS pKT3Ts-goldyTALEN SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 939) AUTHORS Welker JM, Wierson WA, Wang Y, Poshusta TL, McNulty MS, Tisdale EE, Solin SL, Ekker SC, Clark KJ, McGrail M, Essner JJ TITLE GoldyTALEN Vectors with Improved Efficiency for Golden Gate TALEN Assembly. JOURNAL Hum Gene Ther. 2016 Jun;27(6):423-4. doi: 10.1089/hum.2016.012. Epub 2016 May 6. PUBMED 27094430 REFERENCE 2 (bases 1 to 939) AUTHORS . TITLE Direct Submission JOURNAL Exported Jul 19, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..939 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(30..437) /codon_start=1 /gene="lacZ fragment" /product="LacZ-alpha fragment of beta-galactosidase" /label=lacZ-alpha /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGARLQGG TRQCIARCMHCLHCGHAATFCNLHKLACVQL" primer_bind 287..309 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 301..318 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 302..318 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 328..347 /label=T7 /note="T7 promoter, forward primer" promoter 328..346 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(394..414) /label=T3 /note="T3 promoter, forward primer" promoter complement(394..412) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(433..449) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(433..449) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(446..468) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 457..473 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(481..511) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 526..547 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." ORIGIN 1 cggatgcggg ggagttgaga ggtccgccgt tacagttgga cacaggccaa cttgtgaaga 61 ttgcaaaacg tggcggcgtg accgcaatgg aggcagtgca tgcatcgcgc aatgcactga 121 cgggtgcccc cctggagacg ggcgccgcta cagggcgcgt cccattcgcc attcaggctg 181 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa 241 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt 301 tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac tatagggcga attgggtacc 361 gggccccccc tcgaggtcct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt 421 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 481 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 541 cacattaatt gcgttgcgct cactgcccgc tttccaccgg tcgtctccaa cgaccacctc 601 gtcgccttgg cctgcctcgg cggacgtcct gccatggatg cagtgaaaaa gggattgccg 661 cacgcgccgg aattgatcag aagagtcaat cgccgtattg gcgaacgcac gtcccatcgc 721 gttgcctcta gatcccagct agtgaaatct gaattggaag agaagaaatc tgaacttaga 781 cataaattga aatatgtgcc acatgaatat attgaattga ttgaaatcgc aagaaattca 841 actcaggata gaatccttga aatgaaggtg atggagttct ttatgaaggt ttatggttat 901 cgtggtaaac atttgggtgg atcaaggaaa ccagacgga //