LOCUS       Exported               14363 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pRR-PR-5Z
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14363)
  AUTHORS   Miller CA, Tan X, Wilson M, Bhattacharyya S, Ludwig S
  TITLE     Single plasmids expressing human steroid hormone receptors and a 
            reporter gene for use in yeast signaling assays.
  JOURNAL   Plasmid. 2009 Dec 3. ():.
  PUBMED    19962400
REFERENCE   2  (bases 1 to 14363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14363
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(11..28)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(262..281)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(941..1801)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1564..1583
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1802..1906)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    1943..2446
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     2487..2505
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2543..2565)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2665..2684
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        2851..3515
                     /gene="GAL1 and GAL10 genes of S. cerevisiae"
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     protein_bind    3067..3184
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     3479..3502
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     RBS             7309..7320
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      7356..7655
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     promoter        8322..8469
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             8470..9144
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(9247..9702)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9384..9405)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9596..9615
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9828..9850
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9842..9859
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9843..9859
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9866..9885
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9866..9884
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      10037..10224
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     10145..10169
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(14143..14159)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14143..14159)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14156..14178)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14167..14183
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(14191..14221)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14236..14257
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1921 aagtgccacc tgggtccttt tcatcacgtg ctataaaaat aattataatt taaatttttt
     1981 aatataaata tataaattaa aaatagaaag taaaaaaaga aattaaagaa aaaatagttt
     2041 ttgttttccg aagatgtaaa agactctagg gggatcgcca acaaatacta ccttttatct
     2101 tgctcttcct gctctcaggt attaatgccg aattgtttca tcttgtctgt gtagaagacc
     2161 acacacgaaa atcctgtgat tttacatttt acttatcgtt aatcgaatgt atatctattt
     2221 aatctgcttt tcttgtctaa taaatatata tgtaaagtac gctttttgtt gaaatttttt
     2281 aaacctttgt ttattttttt ttcttcattc cgtaactctt ctaccttctt tatttacttt
     2341 ctaaaatcca aatacaaaac ataaaaataa ataaacacag agtaaattcc caaattattc
     2401 catcattaaa agatacgagg cgcgtgtaag ttacaggcaa gcgatccgtc taagaaacca
     2461 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     2521 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     2581 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     2641 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     2701 gatcacgaca ttactatata tataatatag gaagcattta atagaacagc atcgtaatat
     2761 atgtgtactt tgcagttatg acgccagatg gcagtagtgg aagatattct ttattgaaaa
     2821 atagcttgtc accttaccta gaggatcccc tttcaaaaat tcttactttt tttttggatg
     2881 gacgcaaaga agtttaataa tcatattaca tggcattacc accatataca tatccatata
     2941 catatccata tctaatctta cttatatgtt gtggaaatgt aaagagcccc attatcttag
     3001 cctaaaaaaa ccttctcttt ggaactttca gtaatacgct taactgctca ttgctatatt
     3061 gaagtacgga ttagaagccg ccgagcgggt gacagccctc cgaaggaaga ctctcctccg
     3121 tgcgtcctcg tcttcaccgg tcgcgttcct gaaacgcaga tgtgcctcgc gccgcactgc
     3181 tccgaacaat aaagattcta caatactagc ttttatggtt atgaagagga aaaattggca
     3241 gtaacctggc cccacaaacc ttcaaatgaa cgaatcaaat taacaaccat aggatgataa
     3301 tgcgattagt tttttagcct tatttctggg gtaattaatc agcgaagcga tgatttttga
     3361 tctattaaca gatatataaa tgcaaaaact gcataaccac tttaactaat actttcaaca
     3421 ttttcggttt gtattacttc ttattcaaat gtaataaaag tatcaacaaa aaattgttaa
     3481 tatacctcta tactttaacg tcaaggagaa aaaacccggg taccgagctc gaattccacc
     3541 atgactgagc tgaaggcaaa gggtccccgg gctccccacg tggcgggcgg cccgccctcc
     3601 cccgaggtcg gatccccact gctgtgtcgc ccagccgcag gtccgttccc ggggagccag
     3661 acctcggaca ccttgcctga agtttcggcc atacctatct ccctggacgg gctactcttc
     3721 cctcggccct gccagggaca ggacccctcc gacgaaaaga cgcaggacca gcagtcgctg
     3781 tcggacgtgg agggcgcata ttccagagct gaagctacaa ggggtgctgg aggcagcagt
     3841 tctagtcccc cagaaaagga cagcggactg ctggacagtg tcttggacac tctgttggcg
     3901 ccctcaggtc ccgggcagag ccaacccagc cctcccgcct gcgaggtcac cagctcttgg
     3961 tgcctgtttg gccccgaact tcccgaagat ccaccggctg cccccgccac ccagcgggtg
     4021 ttgtccccgc tcatgagccg gtccgggtgc aaggttggag acagctccgg gacggcagct
     4081 gcccataaag tgctgccccg gggcctgtca ccagcccggc agctgctgct cccggcctct
     4141 gagagccctc actggtccgg ggccccagtg aagccgtctc cgcaggccgc tgcggtggag
     4201 gttgaggagg aggatggctc tgagtccgag gagtctgcgg gtccgcttct gaagggcaaa
     4261 cctcgggctc tgggtggcgc ggcggctgga ggaggagccg cggctgtccc gccgggggcg
     4321 gcagcaggag gcgtcgccct ggtccccaag gaagattccc gcttctcagc gcccagggtc
     4381 gccctggtgg agcaggacgc gccgatggcg cccgggcgct ccccgctggc caccacggtg
     4441 atggatttca tccacgtgcc tatcctgcct ctcaatcacg ccttattggc agcccgcact
     4501 cggcagctgc tggaagacga aagttacgac ggcggggccg gggctgccag cgcctttgcc
     4561 ccgccgcgga gttcaccctg tgcctcgtcc accccggtcg ctgtaggcga cttccccgac
     4621 tgcgcgtacc cgcccgacgc cgagcccaag gacgacgcgt accctctcta tagcgacttc
     4681 cagccgcccg ctctaaagat aaaggaggag gaggaaggtg cggaggcctc cgcgcgctcc
     4741 ccgcgttcct accttgtggc cggtgccaac cccgcagcct tcccggattt cccgttgggg
     4801 ccaccgcccc cgctgccgcc gcgagcgacc ccatccagac ccggggaagc ggcggtgacg
     4861 gccgcacccg ccagtgcctc agtctcgtct gcgtcctcct cggggtcgac cctggagtgc
     4921 atcctgtaca aagcggaggg cgcgccgccc cagcagggcc cgttcgcgcc gccgccctgc
     4981 aaggcgccgg gcgcgagcgg ctgcctgctc ccgcgggacg gcctgccctc cacctccgcc
     5041 tctgccgccg ccgccggggc ggcccccgcg ctctaccctg cactcggcct caacgggctc
     5101 ccgcagctcg gctaccaggc cgccgtgctc aaggagggcc tgccgcaggt ctacccgccc
     5161 tatctcaact acctgaggcc ggattcagaa gccagccaga gcccacaata cagcttcgag
     5221 tcattacctc agaagatttg tttaatctgt ggggatgaag catcaggctg tcattatggt
     5281 gtccttacct gtgggagctg taaggtcttc tttaagaggg caatggaagg gcagcacaac
     5341 tacttatgtg ctggaagaaa tgactgcatc gttgataaaa tccgcagaaa aaactgccca
     5401 gcatgtcgcc ttagaaagtg ctgtcaggct ggcatggtcc ttggaggtcg aaaatttaaa
     5461 aagttcaata aagtcagagt tgtgagagca ctggatgctg ttgctctccc acagccattg
     5521 ggcgttccaa atgaaagcca agccctaagc cagagattca ctttttcacc aggtcaagac
     5581 atacagttga ttccaccact gatcaacctg ttaatgagca ttgaaccaga tgtgatctat
     5641 gcaggacatg acaacacaaa acctgacacc tccagttctt tgctgacaag tcttaatcaa
     5701 ctaggcgaga ggcaacttct ttcagtagtc aagtggtcta aatcattgcc aggttttcga
     5761 aacttacata ttgatgacca gataactctc attcagtatt cttggatgag cttaatggtg
     5821 tttggtctag gatggagatc ctacaaacat gtcagtgggc agatgctgta ttttgcacct
     5881 gatctaatac taaatgaaca gcggatgaaa gaatcatcat tctattcatt atgccttacc
     5941 atgtggcaga tcccacagga gtttgtcaag cttcaagtta gccaagaaga gttcctctgt
     6001 atgaaagtat tgttacttct taatacaatt cctttggaag ggctacgaag tcaaacccag
     6061 tttgaggaga tgaggtcaag ctacattaga gagctcatca aggcaattgg tttgaggcaa
     6121 aaaggagttg tgtcgagctc acagcgtttc tatcaactta caaaacttct tgataacttg
     6181 catgatcttg tcaaacagct tcatctgtac tgcttgaata catttatcca gtcccgggca
     6241 ctgagtgttg aatttccaga aatgatgtct gaagttattg ctgcacaatt acccaagata
     6301 ttggcaggga tggtgaaacc ccttctcttt cataaaaagt gaatgtcatc tttttctttt
     6361 aaagaattaa attttgtggc atgtcttttt gttttggtca ggattatgag gtcttgagtt
     6421 tttataatgt tcttctgaaa gccttacatt tataacatca tagtgtgtaa atttaaaaga
     6481 aaaattgtga ggttctaatt attttctttt ataaagtata attagaatgt ttaactgttt
     6541 tgtttaccca tattttcttg aagaatttac aagattgaaa aagtactaaa attgttaaag
     6601 taaactatat cttatccata ttatttcata ccatgtaggt gaggattttt aacttttgca
     6661 tctaacaaat catcgactta agagaaaaaa tcttacatgt aataacacaa agctattata
     6721 tgttatttct aggtaactcc ctttgtgtca attatatttc caaaaatgaa cctttaaaat
     6781 ggtatgcaaa attttgtcta tatatatttg tgtgaggagg aaattcataa ctttcctcag
     6841 attttcaaaa gtatttttaa tgcaaaaaat gtagaaagag tttaaaacca ctaaaataga
     6901 ttgatgttct tcaaactagg caaaacaact catatgttaa gaccattttc cagattggaa
     6961 acacaaatct cttaggaagt taataagtag attcatatca ttatacaaat agtattgtgg
     7021 gttttgtagg tttttaaaat aacctttttt ggggagagaa ttgtcctcta atgaggtatt
     7081 gcgagtggac ataagaaatc agaagattat ggcctaactg tactccttac caactgtggc
     7141 atgctgaaag ttagtcactc ttactgattc tcaattctct cacctttgaa agtagtaaaa
     7201 tatctttcct gccaattgct cctttgggtc agagcttatt aacatctttt caaatcaaag
     7261 gaaagaagaa agggagagga ggaggaggga gatatcaatt cacatacctt tctcctcttt
     7321 atcctccact atcatgaatt cgacactcta aataagcgga tctcttatgt ctttacgatt
     7381 tatagttttc attatcaagt atgcctatat tagtatatag catctttaga tgacagtgtt
     7441 cgaagtttca cgaataaaag ataatattct actttttgct cccaccgcgt ttgctagcac
     7501 gagtgaacac catccctcgc ctgtgagttg tacccattcc tctaaactgt agacatggta
     7561 gcttcagcag tgttcgttat gtacggcatc ctccaacaaa cagtcggtta tagtttgtcc
     7621 tgctcctctg aatcgtctcc ctcgatattt ctcattttcc ttcgcatgcc ctcgagcgga
     7681 acgaagcatc tgtgcttcat tttgtagaac aaaaatgcaa cgcgagagcg ctaatttttc
     7741 aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaaa gcgctatttt
     7801 accaacgaag aatctgtgct tcatttttgt aaaacaaaaa tgcaacgcga gagcgctaat
     7861 ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg cgagagcgct
     7921 attttaccaa caaagaatct atacttcttt tttgttctac aaaaatgcat cccgagagcg
     7981 ctatttttct aacaaagcat cttagattac tttttttctc ctttgtgcgc tctataatgc
     8041 agtctcttga taactttttg cactgtaggt ccgttaaggt tagaagaagg ctactttggt
     8101 gtctattttc tcttccataa aaaaagcctg actccacttc ccgcgtttac tgattactag
     8161 cgaagctgcg ggtgcatttt ttcaagataa aggcatcccc gattatattc tataccgatg
     8221 tggattgcgc atactttgtg aacagaaagt gatagcgttg atgattcttc attggtcaga
     8281 aaattatgaa cggtttcttc tattttgtct ctatatacta cgtacaatct tgatccggag
     8341 cttttctttt tttgccgatt aagaattaat tcggtcgaaa aaagaaaagg agagggccaa
     8401 gagggagggc attggtgact attgagcacg tgagtatacg tgattaagca cacaaaggca
     8461 gcttggagta tgtctgttat taatttcaca ggtagttctg gtccattggt gaaagtttgc
     8521 ggcttgcaga gcacagaggc cgcagaatgt gctctagatt ccgatgctga cttgctgggt
     8581 attatatgtg tgcccaatag aaagagaaca attgacccgg ttattgcaag gaaaatttca
     8641 agtcttgtaa aagcatataa aaatagttca ggcactccga aatacttggt tggcgtgttt
     8701 cgtaatcaac ctaaggagga tgttttggct ctggtcaatg attacggcat tgatatcgtc
     8761 caactgcatg gagatgagtc gtggcaagaa taccaagagt tcctcggttt gccagttatt
     8821 aaaagactcg tatttccaaa agactgcaac atactactca gtgcagcttc acagaaacct
     8881 cattcgttta ttcccttgtt tgattcagaa gcaggtggga caggtgaact tttggattgg
     8941 aactcgattt ctgactgggt tggaaggcaa gagagccccg aaagcttaca ttttatgtta
     9001 gctggtggac tgacgccaga aaatgttggt gatgcgctta gattaaatgg cgttattggt
     9061 gttgatgtaa gcggaggtgt ggagacaaat ggtgtaaaag actctaacaa aatagcaaat
     9121 ttcgtcaaaa atgctaagaa ataggttatt actgagtagt atttatttaa gtattgtttg
     9181 tgcacttgcc gatctatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     9241 atcaggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     9301 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     9361 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     9421 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     9481 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     9541 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     9601 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     9661 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggct
     9721 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     9781 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     9841 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggatccgt
     9901 gtggaagaac gattacaaca ggtgttgtcc tctgaggaca taaaatacac accgagattc
     9961 atcaactcat tgctggagtt agcatatcta caattgggtg aaatggggag cgatttgcag
    10021 gcatttgctc ggcatgccgg tagaggtgtg gtcaataaga gcgacctcat gctatacctg
    10081 agaaagcaac ctgacctaca ggaaagagtt actcaagaat aagaattttc gttttaaaac
    10141 ctaagagtca ctttaaaatt tgtatacact tatttttttt ataacttatt taataataaa
    10201 aatcataaat cataagaaat tcgcttattt agaagtgtca acaacgtatc taccaacgat
    10261 ttgacccttt tccatctttt cgtaaatttc tggcaaggta gacaagccga caaccttgat
    10321 tggagacttg accaaacctc tggcgaagaa gtccaaagct ccaccgcggt ggcggccgtt
    10381 acttacttag agctcgacgt cttacttact tagcggccgc actagtagat ccgtctggta
    10441 cagggtgttc tttttgtctg gtacagggtg ttctttttgt ctggtacagg gtgttctttt
    10501 tgtcgacgtc tggtacaggg tgttcttttt gtctggtaca gggtgttctt tttcccgggg
    10561 tcgacctcga ggcatgtgct ctgtatgtat ataaaactct tgttttcttc ttttctctaa
    10621 atattctttc cttatacatt aggtcctttg tagcataaat tactatactt ctatagacac
    10681 gcaaacacaa atacacacac taaattaata atgaccggat ccggagcttg gctgttgccc
    10741 gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc
    10801 gcgttggccg attcattaat gcagctggca cgacaggttt cccgacttaa tcgccttgca
    10861 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
    10921 caacagttgc gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc accagaagcg
    10981 gtgccggaaa gctggctgga gtgcgatctt cctgaggccg atactgtcgt cgtcccctca
    11041 aactggcaga tgcacggtta cgatgcgccc atctacacca acgtgaccta tcccattacg
    11101 gtcaatccgc cgtttgttcc cacggagaat ccgacgggtt gttactcgct cacatttaat
    11161 gttgatgaaa gctggctaca ggaaggccag acgcgaatta tttttgatgg cgttaactcg
    11221 gcgtttcatc tgtggtgcaa cgggcgctgg gtcggttacg gccaggacag tcgtttgccg
    11281 tctgaatttg acctgagcgc atttttacgc gccggagaaa accgcctcgc ggtgatggtg
    11341 ctgcgctgga gtgacggcag ttatctggaa gatcaggata tgtggcggat gagcggcatt
    11401 ttccgtgacg tctcgttgct gcataaaccg actacacaaa tcagcgattt ccatgttgcc
    11461 actcgcttta atgatgattt cagccgcgct gtactggagg ctgaagttca gatgtgcggc
    11521 gagttgcgtg actacctacg ggtaacagtt tctttatggc agggtgaaac gcaggtcgcc
    11581 agcggcaccg cgcctttcgg cggtgaaatt atcgatgagc gtggtggtta tgccgatcgc
    11641 gtcacactac gtctgaacgt cgaaaacccg aaactgtgga gcgccgaaat cccgaatctc
    11701 tatcgtgcgg tggttgaact gcacaccgcc gacggcacgc tgattgaagc agaagcctgc
    11761 gatgtcggtt tccgcgaggt gcggattgaa aatggtctgc tgctgctgaa cggcaagccg
    11821 ttgctgattc gaggcgttaa ccgtcacgag catcatcctc tgcatggtca ggtcatggat
    11881 gagcagacga tggtgcagga tatcctgctg atgaagcaga acaactttaa cgccgtgcgc
    11941 tgttcgcatt atccgaacca tccgctgtgg tacacgctgt gcgaccgcta cggcctgtat
    12001 gtggtggatg aagccaatat tgaaacccac ggcatggtgc caatgaatcg tctgaccgat
    12061 gatccgcgct ggctaccggc gatgagcgaa cgcgtaacgc gaatggtgca gcgcgatcgt
    12121 aatcacccga gtgtgatcat ctggtcgctg gggaatgaat caggccacgg cgctaatcac
    12181 gacgcgctgt atcgctggat caaatctgtc gatccttccc gcccggtgca gtatgaaggc
    12241 ggcggagccg acaccacggc caccgatatt atttgcccga tgtacgcgcg cgtggatgaa
    12301 gaccagccct tcccggctgt gccgaaatgg tccatcaaaa aatggctttc gctacctgga
    12361 gagacgcgcc cgctgatcct ttgcgaatac gcccacgcga tgggtaacag tcttggcggt
    12421 ttcgctaaat actggcaggc gtttcgtcag tatccccgtt tacagggcgg cttcgtctgg
    12481 gactgggtgg atcagtcgct gattaaatat gatgaaaacg gcaacccgtg gtcggcttac
    12541 ggcggtgatt ttggcgatac gccgaacgat cgccagttct gtatgaacgg tctggtcttt
    12601 gccgaccgca cgccgcatcc agcgctgacg gaagcaaaac accagcagca gtttttccag
    12661 ttccgtttat ccgggcaaac catcgaagtg accagcgaat acctgttccg tcatagcgat
    12721 aacgagctcc tgcactggat ggtggcgctg gatggtaagc cgctggcaag cggtgaagtg
    12781 cctctggatg tcgctccaca aggtaaacag ttgattgaac tgcctgaact accgcagccg
    12841 gagagcgccg ggcaactctg gctcacagta cgcgtagtgc aaccgaacgc gaccgcatgg
    12901 tcagaagccg ggcacatcag cgcctggcag cagtggcgtc tggcggaaaa cctcagtgtg
    12961 acgctccccg ccgcgtccca cgccatcccg catctgacca ccagcgaaat ggatttttgc
    13021 atcgagctgg gtaataagcg ttggcaattt aaccgccagt caggctttct ttcacagatg
    13081 tggattggcg ataaaaaaca actgctgacg ccgctgcgcg atcagttcac ccgtgcaccg
    13141 ctggataacg acattggcgt aagtgaagcg acccgcattg accctaacgc ctgggtcgaa
    13201 cgctggaagg cggcgggcca ttaccaggcc gaagcagcgt tgttgcagtg cacggcagat
    13261 acacttgctg atgcggtgct gattacgacc gctcacgcgt ggcagcatca ggggaaaacc
    13321 ttatttatca gccggaaaac ctaccggatt gatggtagtg gtcaaatggc gattaccgtt
    13381 gatgttgaag tggcgagcga tacaccgcat ccggcgcgga ttggcctgaa ctgccagctg
    13441 gcgcaggtag cagagcgggt aaactggctc ggattagggc cgcaagaaaa ctatcccgac
    13501 cgccttactg ccgcctgttt tgaccgctgg gatctgccat tgtcagacat gtataccccg
    13561 tacgtcttcc cgagcgaaaa cggtctgcgc tgcgggacgc gcgaattgaa ttatggccca
    13621 caccagtggc gcggcgactt ccagttcaac atcagccgct acagtcaaca gcaactgatg
    13681 gaaaccagcc atcgccatct gctgcacgcg gaagaaggca catggctgaa tatcgacggt
    13741 ttccatatgg ggattggtgg cgacgactcc tggagcccgt cagtatcggc ggaattacag
    13801 ctgagcgccg gtcgctacca ttaccagttg gtctggtgtc aaaaataata ataaccgggc
    13861 aggccatgtc tgcccgtatt tcgcgtaagg aaatccatta tgtactattt aaaaaacaca
    13921 aacttttgga tgttcggttt attctttttc ttttactttt ttatcatggg agcctacttc
    13981 ccgtttttcc cgatttggct acatgacatc aaccatatca gcaaaagtga tacgggtatt
    14041 atttttgccg ctatttctct gttctcgcta ttattccaac cgctgtttgg tctgctttct
    14101 gacaaactcg gcctcgactc tagcgaatta attcgtaatc atgtcatagc tgtttcctgt
    14161 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
    14221 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
    14281 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
    14341 aggcggtttg cgtattgggc gct
//
