LOCUS Exported 958 bp ds-DNA linear SYN 23-MAY-2021 DEFINITION used to silence target human FASN expression via RNA interference (3rd gen lentiviral vector). ACCESSION . VERSION . KEYWORDS pLKO.1 puro-humanU6-shRNA FASN SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 958) TITLE Elizabeth Stoll plasmids JOURNAL Unpublished REFERENCE 2 (bases 1 to 958) AUTHORS . TITLE Direct Submission JOURNAL Exported May 23, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..958 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 117..234 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter complement(340..580) /label=U6 promoter /note="RNA polymerase III promoter for human U6 snRNA" primer_bind complement(390..409) /label=LKO.1 5' /note="Human U6 promoter, forward primer" primer_bind complement(560..580) /label=hU6-F /note="Human U6 promoter, forward primer" CDS complement(878..922) /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" ORIGIN 1 cacgcccaga gcagccgcgt ccctgcgcaa acccagggct gccttggaaa aggcgcaacc 61 ccaaccccct cgagccgcgg ccaaagtgga tctctgctgt ccctgtaata aacccgaaaa 121 ttttgaattt ttgtaatttg tttttgtaat tctttagttt gtatgtctgt tgctattatg 181 tctactattc tttcccctgc actgtacccc ccaatccccc cttttctttt aaaattgtgg 241 atgaatactg ccatttgtct cgaggtcgag aattcaaaaa cgagagcacc tttgatgaca 301 tctcgagatg tcatcaaagg tgctctcgac cggtgtttcg tcctttccac aagatatata 361 aagccaagaa atcgaaatac tttcaagtta cggtaagcat atgatagtcc attttaaaac 421 ataattttaa aactgcaaac tacccaagaa attattactt tctacgtcac gtattttgta 481 ctaatatctt tgtgtttaca gtcaaattaa ttccaattat ctctctaaca gccttgtatc 541 gtatatgcaa atatgaagga atcatgggaa ataggccctc ggtgaagggg gcggccgctc 601 gaggctagtc tcgtgatcga taccgtcgag atccgttcac taatcgaatg gatctgtctc 661 tgtctctctc tccaccttct tcttctattc cttcgggcct gtcgggtccc ctcggggttg 721 ggaggtgggt ctgaaacgat aatggtgaat atccctgcct aactctattc actatagaaa 781 gtacagcaaa aactattctt aaacctacca agcctcctac tatcattatg aataatttta 841 tataccacag ccaatttgtt atgttaaacc aattccacaa acttgcccat ttatctaatt 901 ccaataattc ttgttcattc ttttcttgct ggttttgcga ttcttcaatt aaggagtg //