LOCUS       Exported                 233 bp ds-DNA     linear   SYN 23-MAY-2021
DEFINITION  used to silence target human FASN expression via RNA interference 
            (3rd gen lentiviral vector).
ACCESSION   .
VERSION     .
KEYWORDS    pLKO.1 puro-humanU6-shRNA FASN
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 233)
  TITLE     Elizabeth Stoll plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 233)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 23, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     128..147
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 caaggctgtt agagagataa ttggaattaa tttgactgta aacacaaaga tattagtaca
       61 aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt
      121 taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata
      181 tatcttgtgg aaaggacgaa acaccggtcg agagcacctt tgatgacatc tcg
//
