LOCUS       Exported                 816 bp ds-DNA     linear   SYN 03-AUG-2021
DEFINITION  Encodes the G protein to enable transsynaptic RAV infection.
ACCESSION   .
VERSION     .
KEYWORDS    pAM-CAGGS-FLEX-H2B-3XHA-P2A-N2c(G)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Reardon TR, Murray AJ, Turi GF, Wirblich C, Croce KR, Schnell MJ, 
            Jessell TM, Losonczy A
  TITLE     Rabies Virus CVS-N2c Strain Enhances Retrograde Synaptic Transfer 
            and Neuronal Viability.
  JOURNAL   Neuron. 2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 
            10.1016/j.neuron.2016.01.004.
  PUBMED    26804990
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 3, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    complement(61..94)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     protein_bind    complement(165..198)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites (Lee and 
                     Saito, 1988)."
     CDS             226..603
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSSK"
     CDS             622..711
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             721..777
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 ttttgtaatc cagaggttga ttatcgataa gctgttaaag cttgaacctc ttcgagggac
       61 ataacttcgt ataatgtatg ctatacgaag ttatgggtcg atggtgagat ctggactaga
      121 gggtcgatgg tgatgcttgg atcaaaatag gaagaatccc aatgataact tcgtataaag
      181 tatcctatac gaagttatga tatcagatct ctcgaggccg ccaccatgcc agagccagcg
      241 aagtctgctc ccgccccgaa aaagggctcc aagaaggcgg tgactaaggc gcagaagaaa
      301 ggcggcaaga agcgcaagcg cagccgcaag gagagctatt ccatctatgt gtacaaggtt
      361 ctgaagcagg tccaccctga caccggcatt tcgtccaagg ccatgggcat catgaactcg
      421 tttgtgaacg acattttcga gcgcatcgca ggtgaggctt cccgcctggc gcattacaac
      481 aagcgctcga ccatcacctc cagggagatc cagacggccg tgcgcctgct gctgcctggg
      541 gagttggcca agcacgccgt gtccgagggt actaaggcca tcaccaagta caccagctct
      601 aaggatggat ccggcgccgt ttacccatac gatgttcctg actatgcggg ctatccctat
      661 gacgtcccgg actatgcagg atcctatcca tatgacgttc cagattacgc tggaagcgga
      721 gctactaact tcagcctgct gaagcaggct ggagacgtgg aggagaaccc tggacctatg
      781 gttcctcagg ttcttttgtt tgtactcctt ctgggt
//
