LOCUS Exported 986 bp ds-DNA linear SYN 08-JUN-2021 DEFINITION mammalian expression of LOV V416I. ACCESSION . VERSION . KEYWORDS pTriEx-mCherry-LOV2 V416I SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 986) AUTHORS Wang H, Vilela M, Winkler A, Tarnawski M, Schlichting I, Yumerefendi H, Kuhlman B, Liu R, Danuser G, Hahn KM TITLE LOVTRAP: an optogenetic system for photoinduced protein dissociation. JOURNAL Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926. PUBMED 27427858 REFERENCE 2 (bases 1 to 986) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 8, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..986 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 219..241 /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" primer_bind 303..322 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" primer_bind 364..383 /label=T7 /note="T7 promoter, forward primer" promoter 364..382 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind 383..407 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter 423..532 /label=p10 promoter /note="baculovirus promoter for expression in insect cells" primer_bind complement(692..710) /label=mCherry-R /note="mCherry, reverse primer" ORIGIN 1 ctatataagc agacgtcgtt tagtgaaccg tcagatcact agatgcttta ttgcggtagt 61 ttatcacagt taaattgcta acgccagtct cgaacttaac gtgcagaagt tggtcgtgag 121 gcactgggca ggtaagtatc gggccctttg tgcgggggga gcggctcggg gctgtccgcg 181 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac 241 cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct 301 gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaattgg atcggaccga 361 aattaatacg actcactata ggggaattgt gagcggataa caattccccg gagttaatcc 421 gggaccttta attcaaccca acacaatata ttatagttaa ataagaatta ttatcaaatc 481 atttgtatat taattaaaat actatactgt aaattacatt ttatttacaa tcaaaggaga 541 tataccatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc 601 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc 661 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc 721 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg 781 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg 841 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg 901 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc 961 cctgtaatgc agaagaagac tatggg //