LOCUS Exported 7463 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION High copy number plasmid containing Ple253 (PITX3 MiniPromoter) insert.. ACCESSION . VERSION . KEYWORDS pEMS1920 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7463) AUTHORS Korecki AJ, Hickmott JW, Lam SL, Dreolini L, Mathelier A, Baker O, Kuehne C, Bonaguro RJ, Smith J, Tan CV, Zhou M, Goldowitz D, Deussing JM, Stewart AF, Wasserman WW, Holt RA, Simpson EM TITLE Twenty-Seven Tamoxifen-Inducible iCre-Driver Mouse Strains for Eye and Brain; Including Seventeen Carrying a New Inducible-First Constitutive-Ready Allele. JOURNAL Genetics. 2019 Feb 14. pii: genetics.119.301984. doi: 10.1534/genetics.119.301984. PUBMED 30765420 REFERENCE 2 (bases 1 to 7463) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7463 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(496..513) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(496..512) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(611..628) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(782..1370) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(862..881) /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2741..2760 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" CDS complement(3645..4505) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 4268..4287 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(4506..4597) /gene="bla" /label=AmpR promoter primer_bind 4664..4683 /label=pENTR-R /note="pENTR vectors, reverse primer" ORIGIN 1 ggtctcctat acgtctctta tacgacatca ccgatgggga acccagacgc tgagtacgta 61 ttctaaatgc ataataaata ctgataacat cttatagttt gtattatatt ttgtattatc 121 gttgacatgt ataattttga tatcaaaaac tgattttccc tttattattt tcgagattta 181 ttttcttaat tctctttaac aaactagaaa tattgtatat acaaaaaatc ataaataata 241 gatgaatagt ttaattatag gtgttcatca atcgaaaaag caacgtatct tatttaaagt 301 gcgttgcttt tttctcattt ataaggttaa ataattctca tatatcaagc aaagtgacag 361 gcgcccttaa atattctgac aaatgctctt tccctaaact ccccccataa aaaaacccgc 421 cgaagcgggt ttttacgtta tttgcggatt aacgattact cgttatcaga accgcccagg 481 gggcccgagc ttaagactgg ccgtcgtttt acaacacaga aagagtttgt agaaacgcaa 541 aaaggccatc cgtcaggggc cttctgctta gtttgatgcc tggcagttcc ctactctcgc 601 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 661 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 721 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 781 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 841 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 901 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 961 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 1021 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 1081 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 1141 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggg 1201 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta 1261 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 1321 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 1381 tgatcttttc tacggggtct gacgctcagt ggaacgacgc gcgcgtaact cacgttaagg 1441 gattttggtc atgagcttgc gccgtcccgt caagtcagcg taatgctctg cttaggtggc 1501 ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag 1561 agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc 1621 gttggcctca tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc 1681 gccggagact gcgagatcat agatatagat ctcactacgc ggctgctcaa acttgggcag 1741 aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa 1801 tgtcttacta cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt 1861 ggctacgtca ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg 1921 gtcagggccg agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt 1981 agggcgactg ccctgctgcg taacatcgtt gctgctccat aacatcaaac atcgacccac 2041 ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtacaaaaa aacagtcata 2101 acaagccatg aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca aggttctgga 2161 ccagttgcgt gagcgcattt ttttttcctc ctcggcgttt acgccccgcc ctgccactca 2221 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acagacggca 2281 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc 2341 atagtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg 2401 aaactcaccc agggattggc gctgacgaaa aacatattct caataaaccc tttagggaaa 2461 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg 2521 aaatcgtcgt gtgcactcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac 2581 cagctcaccg tctttcattg ccatacggaa ctccggatga gcattcatca ggcgggcaag 2641 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc 2701 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc 2761 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt 2821 ctccattttt ttttcctcct ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta 2881 ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa 2941 aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact 3001 cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag 3061 aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg catttctttc 3121 cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa 3181 ccgttattca ttcgtgattg cgcctgagcg aggcgaaata cgcgatcgct gttaaaagga 3241 caattacaaa caggtgcaca ctgccagcgc atcaacaata ttttcacctg aatcaggata 3301 ttcttctaat acctggaacg ctgtttttcc ggggatcgca gtggtgagta accatgcatc 3361 atcaggagta cggataaaat gcttgatggt cggaagtggc ataaattccg tcagccagtt 3421 tagtctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa 3481 caactctggc gcatcgggct tcccatacaa gcgatagatt gtcgcacctg attgcccgac 3541 attatcgcga gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg 3601 cctcgacgtt tcccgttgaa tatggctcat ttttttttcc tcctttacca atgcttaatc 3661 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 3721 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagcgc tgcgatgata 3781 ccgcgagaac cacgctcacc ggctccggat ttatcagcaa taaaccagcc agccggaagg 3841 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 3901 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccatcgct 3961 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 4021 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 4081 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 4141 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 4201 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 4261 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 4321 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 4381 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 4441 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 4501 ctcatattct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 4561 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggtcagtgt tacaaccaat 4621 taaccaattc tgaacattat cgcgagccca tttatacctg aatatggctc ataacacccc 4681 ttgtttgcct ggcggcagta gcgcggtggt cccacctgac cccatgccga actcagaagt 4741 gaaacgccgt agcgccgatg gtagtgtggg gactccccat gcgagagtag ggaactgcca 4801 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgcccg ggctaattag 4861 gggggctgga tcgcttcgtg ttccccatcg gtgatgtcgt ataggaagca gtatacgaga 4921 cctataggag acgtatatgg tcttcttttg gcgcgcccgg ctgcggcggc gatctagagg 4981 gcaggcaggg gccaggggcc gggcacccgg ccggagtggg ggccgccccc ctgctcccgg 5041 gccgcctctc cgctcgggcg ctcctggact ctcggaggga gtgagcctca ccgcgtactg 5101 ccacccccag ccggcgccca ttcactttat ggcagaccag ggcgccccca gcccgccgcg 5161 gcgagccgcg cgcgtcaggc cccgcccctt tccagctgtc ctgctggggc tccgcccttt 5221 ccagctgtgg atctccaggc cccgcctttg agggaggggt ctggccggcg agacgccaag 5281 aaccccgccc tctggccaat cagaagcgct cttcagcaac tcggccgcgc ccctccccac 5341 gtggcagaga ccgcgctccg gctaggacgc ttaggcagag ccaagtgggc gagagtagag 5401 tggtcccggt agccacgggt aaaagggatc ggctggcagc gaagtgggtt gggccgctac 5461 cccgaggaaa gtggatcggc gccaggtggg aaggcgcacg gggccgccgg gtctcagcgc 5521 tcagacttct ctgccactca agtttcgccg attttctcca cttgtcgctg tccgtctctc 5581 ctcccttttg tctctgttcc aatcctccca cgtctcgcct ttctttcctc ttctttctcc 5641 tcagccgctt cgctcctgct gtttaccatt ctccttcctc tgccacttta aaagactcat 5701 ttctctttct ctctgctcct ctttcctcat atctttcctc ttcttcattt cccccttctt 5761 ctccaaggaa ggtgacttcg tggagacagc agggtttgga atgatacaaa ccaaagtagt 5821 attccaacgc tgccacgaac tgcaccatca ctgagtgaac ttggacgagt aactatcatc 5881 tccctccccg cgcaaaacaa ttaagaggag gttcctgctg gatctcaggg cttgggctgg 5941 gcactccaaa ggcattgcct ttcagtctgt acccttgtct tgcttaggct gtgctctccc 6001 tccttcagca gggctggttt ctcgctggtg gtaccaggtt aagtcctgtg ggcaaggtgt 6061 ggccttggtg cctgctggtt aggaatttgc aagggacagg acagatgacc aaggaacatc 6121 ctggcagact gtgacttcgg ccctctctga cttaggcctc aatttccagg aaacaggctc 6181 cttctcttct cccagttagg ctatgttgct tgggtgctcc gaagagccta agcccaggcc 6241 agacactgtg gctcatgcct gtaatcccag cactttggga ggccgaggcg ggcggatcac 6301 aaggtcagga gattgagacc accctggcta acacggtgaa accccatctc tactaaaaaa 6361 tacaaaaaat tagccgggct tggtggcggg tgcctgtagt ctcagctact ggggaggctg 6421 aggaagaaga atggcgtgaa cccaggaggc agagcttgca gtgagctgag atcgcgccac 6481 tgcactccag actgggcgac agagcaagac tctgtctcaa aaaaaaaaaa aagagcctaa 6541 gcccagtcac acaatatgcc cctaaggcaa atctgagcag tcatgtcttg actgttcagt 6601 gactcccatt gcccttagga taaaggtcaa gctccctagc ctggcataca agaccttcag 6661 gacctgacct tgtcttcatc tccaatttcc tagtagtgtc ctgcacacct ctggcccttc 6721 ctgtgaaagt aacccatctt tatattgtca attcctactc ctccaatgtt ccactcaagt 6781 cttattttct ctgggaagcc ttttctgaac tctcagttta gccccttgct ctcatgaccc 6841 tcagtgtata ccttctatta caccacccac catattgaag ggatcagctt atgtgttgtc 6901 tgctatgata ataaaattta ggagggtaag gaccatgtct gttattccca ggcataatgt 6961 agaatctcaa taaatgtcac actgaaatct atgaggaaga tgttctgcct ggtaagatga 7021 tgattagtgg ttaggaggga ctagggaatt gtcatctctc tcctccccat ctctattgca 7081 caatcacatg cttttagaat gatgaatttc tggaggtatg tagaagtaga tattggcgag 7141 cagaaatttt tggcccactt agaggatttt ccaatcggct ttccaggacc tctcaatctg 7201 agacaggccc aggacaccaa ctctcagttt tcaaatgaag aggaaaatgg ctttgaggta 7261 atgtggaaag aggtgctatg atggtatatc ctctttccct gactctcttt gggtgataga 7321 ggctagatcc tcagctggac ccaccgacca gaagcccaat gtgcaggtgt aggaagttac 7381 tgaggggttc ttcacgtctg atatctgatg ggtgctttac actagagcat cgtggccagg 7441 tggccggccc cgaagacact ata //