LOCUS       Exported                 907 bp ds-DNA     linear   SYN 22-MAY-2021
DEFINITION  MCS for cloning promoter to drive HA-tagged biotin ligase (BirA) 
            with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered 
            mCherry protein (membCherry); flanked by Tol2 sequences.
ACCESSION   .
VERSION     .
KEYWORDS    pMT-MCS-NLS-BirA-2A-mCherry_Ras
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Trinh LA, Chong-Morrison V, Gavriouchkina D, Hochgreb-Hagele T, 
            Senanayake U, Fraser SE, Sauka-Spengler T
  TITLE     Biotagging of Specific Cell Populations in Zebrafish Reveals Gene 
            Regulatory Logic Encoded in the Nuclear Transcriptome.
  JOURNAL   Cell Rep. 2017 Apr 11;19(2):425-440. doi: 
            10.1016/j.celrep.2017.03.045.
  PUBMED    28402863
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 22, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        291..309
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     291..308
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      371..380
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             494..514
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 caagaaccag aggtgtaaag tacttgagta attttacttg attactgtac ttaagtatta
       61 tttttgggga tttttacttt acttgagtac aattaaaaat caatactttt acttttactt
      121 aattacattt ttttagaaaa aaaagtactt tttactcctt acaattttat ttacagtcaa
      181 aaagtactta ttttttggag atcacttggg cccggctcga gctacgtact taagcttgag
      241 gatcccatcg attcgaattc tcggtaccta gtcgaccttc gcgaaggcct atttaggtga
      301 cactatagaa gagtacaatt caaggttaag cggccgcaag ctagattggc attccggtac
      361 tgttggtaaa gccaccatgg ccacctatga cgtcccagat tacgcaagtt tgcccgggta
      421 tgatgttcct gattatgcta gcctcccggg ttacgatgtg cccgactatg cctcacttcc
      481 aggcagatct cctccaaaaa agaagagaaa ggtacgatct atgaaggata acaccgtgcc
      541 actgaaattg attgccctgt tagcgaacgg tgaatttcac tctggcgagc agttgggtga
      601 aacgctggga atgagccggg cggctattaa taaacacatt cagacactgc gtgactgggg
      661 cgttgatgtc tttaccgttc cgggtaaagg atacagcctg cctgagccta tccagttact
      721 taatgctaaa cagatattgg gtcagctgga tggcggtagt gtagccgtgc tgccagtgat
      781 tgactccacg aatcagtacc ttcttgatcg tatcggagag cttaaatcgg gcgatgcttg
      841 cattgcagaa taccagcagg ctggccgtgg tcgccggggt cggaaatggt tttcgccttt
      901 tggcgca
//
