LOCUS Exported 998 bp ds-DNA linear SYN 30-MAY-2021 DEFINITION Sox10 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences. ACCESSION . VERSION . KEYWORDS pMT-sox10-NLS-BirA-2A-mCherry_Ras SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 998) AUTHORS Trinh LA, Chong-Morrison V, Gavriouchkina D, Hochgreb-Hagele T, Senanayake U, Fraser SE, Sauka-Spengler T TITLE Biotagging of Specific Cell Populations in Zebrafish Reveals Gene Regulatory Logic Encoded in the Nuclear Transcriptome. JOURNAL Cell Rep. 2017 Apr 11;19(2):425-440. doi: 10.1016/j.celrep.2017.03.045. PUBMED 28402863 REFERENCE 2 (bases 1 to 998) AUTHORS . TITLE Direct Submission JOURNAL Exported May 30, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..998 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 104..124 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" ORIGIN 1 cgtcccagat tacgcaagtt tgcccgggta tgatgttcct gattatgcta gcctcccggg 61 ttacgatgtg cccgactatg cctcacttcc aggcagatct cctccaaaaa agaagagaaa 121 ggtacgatct atgaaggata acaccgtgcc actgaaattg attgccctgt tagcgaacgg 181 tgaatttcac tctggcgagc agttgggtga aacgctggga atgagccggg cggctattaa 241 taaacacatt cagacactgc gtgactgggg cgttgatgtc tttaccgttc cgggtaaagg 301 atacagcctg cctgagccta tccagttact taatgctaaa cagatattgg gtcagctgga 361 tggcggtagt gtagccgtgc tgccagtgat tgactccacg aatcagtacc ttcttgatcg 421 tatcggagag cttaaatcgg gcgatgcttg cattgcagaa taccagcagg ctggccgtgg 481 tcgccggggt cggaaatggt tttcgccttt tggcgcaaac ttatatttgt cgatgttctg 541 gcgtctggaa caaggcccgg cggcggcgat tggtttaagt ctggttatcg gtatcgtgat 601 ggcggaagta ttacgcaagc tgggtgcaga taaagttcgt gttaaatggc ctaatgacct 661 ctatctgcag gatcgcaagc tggcaggcat tctggtggag ctgactggca aaactggcga 721 tgcggcgcaa atagtcattg gagccgggat caacatggca atgcgccgtg ttgaagagag 781 tgtcgttaat caggggtgga tcacgctgca ggaagcgggg atcaatctcg atcgtaatac 841 gttggcggcc atgctaatac gtgaattacg tgctgcgttg gaactcttcg aacaagaagg 901 attggcacct tatctgtcgc gctgggaaaa gctggataat tttattaatc gcccagtgaa 961 acttatcatt ggtgataaag aaatatttgg catttcac //