LOCUS Exported 888 bp ds-DNA linear SYN 25-MAY-2021 DEFINITION Beta-actin2 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences. ACCESSION . VERSION . KEYWORDS pMTB2-NLS-BirA-2A-mCherry_Ras SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 888) AUTHORS Trinh LA, Chong-Morrison V, Gavriouchkina D, Hochgreb-Hagele T, Senanayake U, Fraser SE, Sauka-Spengler T TITLE Biotagging of Specific Cell Populations in Zebrafish Reveals Gene Regulatory Logic Encoded in the Nuclear Transcriptome. JOURNAL Cell Rep. 2017 Apr 11;19(2):425-440. doi: 10.1016/j.celrep.2017.03.045. PUBMED 28402863 REFERENCE 2 (bases 1 to 888) AUTHORS . TITLE Direct Submission JOURNAL Exported May 25, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..888 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 73..82 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 196..216 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" ORIGIN 1 caggatccca tcgattcgaa ttcaaggtta agcggccgca agctagattg gcattccggt 61 actgttggta aagccaccat ggccacctat gacgtcccag attacgcaag tttgcccggg 121 tatgatgttc ctgattatgc tagcctcccg ggttacgatg tgcccgacta tgcctcactt 181 ccaggcagat ctcctccaaa aaagaagaga aaggtacgat ctatgaagga taacaccgtg 241 ccactgaaat tgattgccct gttagcgaac ggtgaatttc actctggcga gcagttgggt 301 gaaacgctgg gaatgagccg ggcggctatt aataaacaca ttcagacact gcgtgactgg 361 ggcgttgatg tctttaccgt tccgggtaaa ggatacagcc tgcctgagcc tatccagtta 421 cttaatgcta aacagatatt gggtcagctg gatggcggta gtgtagccgt gctgccagtg 481 attgactcca cgaatcagta ccttcttgat cgtatcggag agcttaaatc gggcgatgct 541 tgcattgcag aataccagca ggctggccgt ggtcgccggg gtcggaaatg gttttcgcct 601 tttggcgcaa acttatattt gtcgatgttc tggcgtctgg aacaaggccc ggcggcggcg 661 attggtttaa gtctggttat cggtatcgtg atggcggaag tattacgcaa gctgggtgca 721 gataaagttc gtgttaaatg gcctaatgac ctctatctgc aggatcgcaa gctggcaggc 781 attctggtgg agctgactgg caaaactggc gatgcggcgc aaatagtcat tggagccggg 841 atcaacatgg caatgcgccg tgttgaagag agtgtcgtta atcagggg //