LOCUS       Exported                8710 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pBabe EGFR Del1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8710)
  AUTHORS   Yuza Y, Glatt KA, Jiang J, Greulich H, Minami Y, Woo MS, Shimamura 
            T, Shapiro G, Lee JC, Ji H, Feng W, Chen TH, Yanagisawa H, Wong KK, 
            Meyerson M
  TITLE     Allele-dependent variation in the relative cellular potency of 
            distinct EGFR inhibitors.
  JOURNAL   Cancer Biol Ther. 2007 May . 6(5):661-7.
  PUBMED    17495523
REFERENCE   2  (bases 1 to 8710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8710
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             120..589
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(810..1409)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(893..913)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     1390..1409
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(1419..1748)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1433..1568
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1487..1506)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     1733..1753
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(5435..5851)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(5451..5467)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(5483..5505)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    6052..6251
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             6316..6785
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        6816..6920
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6921..7781
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7139..7158)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7941..8529
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8430..8449
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8683..8700
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcaactatg
       61 gccatttaat gtaaatactt aagaaaaaaa accaaattaa ttttgataca tgctgcatgt
      121 gaaagacccc cgctgacggg tagtcaatca ctcagaggag accctcccaa ggaacagcga
      181 gaccacaagt cggatgcaac tgcaagaggg tttattggat acacgggtac ccgggcgact
      241 cagtcaatcg gaggactggc gccccgagtg aggggttgtg ggctctttta ttgagctcgg
      301 ggagcagaag cgcgcgaaca gaagcgagaa gcgaactgat tggttagttc aaataaggca
      361 cagggtcatt tcaggtcctt ggggcaccct ggaaacatct gatggttctc tagaaactgc
      421 tgagggctgg accgcatctg gggaccatct gttcttggcc ctgagccggg gcaggaactg
      481 cttaccacag atatcctgtt tggcccatat tcagctgttc catctgttct tggccctgag
      541 ccggggcagg aactgcttac cacagatatc ctgtttggcc catattcagc tgttccatct
      601 gttcctgacc ttgatctgaa cttctctatt ctcagttatg tatttttcca tgccttgcaa
      661 aatggcgtta cttaagctag cttgccaaac ctacaggtgg ggtctttcat tccccccttt
      721 ttctggagac taaataaaat cttttatttt atcgatgcat ggggtcgtgc gctcctttcg
      781 gtcgggcgct gcgggtcgtg gggcgggcgt caggcaccgg gcttgcgggt catgcaccag
      841 gtgcgcggtc cttcgggcac ctcgacgtcg gcggtgacgg tgaagccgag ccgctcgtag
      901 aaggggaggt tgcggggcgc ggaggtctcc aggaaggcgg gcaccccggc gcgctcggcc
      961 gcctccactc cggggagcac gacggcgctg cccagaccct tgccctggtg gtcgggcgag
     1021 acgccgacgg tggccaggaa ccacgcgggc tccttgggcc ggtgcggcgc caggaggcct
     1081 tccatctgtt gctgcgcggc cagccgggaa ccgctcaact cggccatgcg cgggccgatc
     1141 tcggcgaaca ccgcccccgc ttcgacgctc tccggcgtgg tccagaccgc caccgcggcg
     1201 ccgtcgtccg cgacccacac cttgccgatg tcgagcccga cgcgcgtgag gaagagttct
     1261 tgcagctcgg tgacccgctc gatgtggcgg tccggatcga cggtgtggcg cgtggcgggg
     1321 tagtcggcga acgcggcggc gagggtgcgt acggccctgg ggacgtcgtc gcgggtggcg
     1381 aggcgcaccg tgggcttgta ctcggtcatg gtaagctttt tgcaaaagcc taggcctcca
     1441 aaaaagcctc ctcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca
     1501 taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg
     1561 ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt
     1621 tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg
     1681 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac
     1741 tgacacacat tccacagggt cgaccactgt gctggcgaat tcctacatct catgctccaa
     1801 taaattcact gctttgtggc gcgaccctta ggtattctgc attttcagct gtggagccct
     1861 taaagatgcc atttggcttg gcttccttgg gaaagaagtc ctgctggtag tcagggttgt
     1921 ccaggctaat ttggtggctg cctttctggg cccagtgggc agggctgtcg aatgtgctgt
     1981 tgacacaggt gggctggaca gtgttgagat actcggggtt gcccactgca gtgctgtggg
     2041 ggtcctggta gtgtgggtct ctgctgggcg cggggttcag aggctgattg tgatagacag
     2101 gattctgcac agagccagcg ggccttttgg gaacggactg gtttatgtat tcaggcactg
     2161 ggaggaaggt gtcgtctatg ctgtcctcag tcaaggcgcc tgtggggtct gagctgtatc
     2221 gctgcaagaa gctgtcttcc ttgatgggac agctttgcag cccatttcta tcaatgcaag
     2281 ccacggtgga attgttgctg gttgcactca gagagctcag gaggggagtc cgtgacgtgg
     2341 aggggctgct gaagaagccc tgctgtggga tgaggtactc gtcggcatcc accacgtcgt
     2401 ccatgtcttc ttcatccatc agggcacggt agaagttgga gtctgtagga cttggcaaat
     2461 gcattctttc atccccctga atgacaaggt agcgctgggg gtctcgggcc attttggaga
     2521 attcgatgat caactcacgg aactttgggc gactatctgc gtctatcatc cagcacttga
     2581 ccatgatcat gtagacatcg atggtacata tgggtggctg agggaggcgt tctcctttct
     2641 ccaggatgga ggagatctcg ctggcaggga ttccgtcata tggcttggat ccaaaggtca
     2701 tcaactccca aacggtcacc ccgtagctcc agacatcact ctggtgggta tagattctgt
     2761 gtaaaattga ttccaatgcc atccacttga taggcacttt gcctccttct gcatggtatt
     2821 ctttctcttc cgcacccagc agtttggcca gcccaaaatc tgtgatcttg acatgctgcg
     2881 gtgttttcac cagtacgttc ctggctgcca ggtcgcggtg caccaagcga cggtcctcca
     2941 agtagttcat gccctttgcg atctgcacac accagttgag caggtactgg gagccaatat
     3001 tgtctttgtg ttcccggaca tagtccagga ggcagccgaa gggcatgagc tgcgtgatga
     3061 gttgcacggt ggaggtgagg cagatgccca gcaggcggca cacgtggggg ttgtccacgc
     3121 tggccatcac gtaggcttca tcgaggattt ccttgttggc tttcggagat gttttgatag
     3181 cgacgggaat tttaactttc tcaccttctg ggatccagag tcccttatac accgtgccga
     3241 acgcaccgga gcccagcact ttgatctttt tgaattcagt ttccttcaag atcctcaaga
     3301 gagcttggtt gggagcttct ccactgggtg taagaggctc cacaagctcc ctctcctgca
     3361 gcagcctccg cagcgtgcgc ttccgaacga tgtggcgcct tcgcatgaag aggccgatcc
     3421 ccagggccac caccagcagc aagaggaggg cccccaccat cccagtggcg atggacggga
     3481 tcttaggccc attcgttgga cagccttcaa gacctggccc agtgcatccg taggtgcagt
     3541 ttggatggca caggtggcac acatggccgg cgtctgcgta cttccagacc agggtgttgt
     3601 tttctcccat gactcctgcc gggcaggtct tgacgcagtg ggggccgtca atgtagtggg
     3661 cacactggat acagttgtct ggtccccgtc ctgtgcaggt gatgttcatg gcctgaggca
     3721 ggcactctgg gtggcactgt atgcactcag agttctccac aaactccctt ggctcaccct
     3781 ccagaaggtt gcacttgtcc acgcattccc tgcctcggct gacattccgg caagagacgc
     3841 agtccctggg ctccgggccc cagcagccct cgggggagca caaggcatgg cagacctggc
     3901 ctgtggcctt gcagctgttt tcacctctgt tgcttataat tttggttttc tgaccggagg
     3961 tcccaaacag ttttttccag tttattgtat ttgcatagca caaatttttg tttcctgaaa
     4021 ttatcacatc tccatcactt atctccttga gggagcgtaa tcccaaggat gttatgttca
     4081 ggctgacgac tgcaagagaa aactgaccat gttgcttggt cctgccgcgt atgatttcta
     4141 ggttctcaaa ggcatggagg tccgtcctgt tttcaggcca agcctgaatc agcaaaaacc
     4201 ctgtgatttc ctttacggtt ttcagaatat ccagttcctg tggatccaga ggaggagtat
     4261 gtgtgaagga gtcaccccta aatgccaccg gcaggatgtg gagatcgcca ctgatggagg
     4321 tgcagttttt gaagtgttta atattcgtag catttatgga gagtgagtct ttaaattcac
     4381 caatacctat tccgttacac actttgcggc aaggcccttc gcacttctta cacttgcgga
     4441 cgccgtcttc ctccatctca tagctgtcgg ccccacaggc tcggacgcac gagccgtgat
     4501 ctgtcaccac ataattacgg ggacacttct tcacgcaggt ggcaccaaag ctgtatttgc
     4561 cctcggggtt cacatccatc tggtacgtgg tggggttgta gagcatgagt ggggggcagg
     4621 tgtccttgca cgtggcttcg tctcggaatt tgcggcagac caggcagtcg ctctcccggg
     4681 ggcctgtgca gcctgcagca cactggttgt ggcagcagtc actgggggac ttgccacggc
     4741 agcgcccgga gcactgctgg gcacagatga ttttggtcag tttctggcag ttctcctctc
     4801 ctgcacccca gcagctccca ttgggacagc ttggatcaca cttttggcag ctgcccaggt
     4861 ggttctggaa gtccatcgac atgttgctga gaaagtcact gctgactatg tcccgccact
     4921 ggatgctctc cacgttgcac agggcagggt tgttgctgaa ccgcacggcg ccatgcagga
     4981 tttcctgtaa atttctcatg ggcagctcct tcagtccggt tttatttgca tcatagttag
     5041 ataagactgc taaggcatag gaattttcgt agtacatatt tcctctgatg atctgcaggt
     5101 tttccaaagg aattcgctcc actgtgttga gggcaatgag gacataacca gccacctcct
     5161 ggatggtctt taagaaggaa agatcataat tcctctgcac ataggtaatt tccaaattcc
     5221 caaggaccac ctcacagtta ttgaacatcc tctggaggct gagaaaatga tcttcaaaag
     5281 tgcccaactg cgtgagcttg ttactcgtgc cttggcaaac tttcttttcc tccagagccc
     5341 gactcgccgg gcagagcgca gccagcagcg ccaggagcgc tgccccggcc gtcccggagg
     5401 gtcgcatgat gtaccaccac actgggatcc ggccggcgcc tagagaagga gtgagggctg
     5461 gataaaggga ggatcgaggc ggggtcgaac gaggaggttc aagggggaga gacggggcgg
     5521 atggaggaag aggaggcgga ggcttagggt gtacaaaggg cttgacccag ggaggggggt
     5581 caaaagccaa ggcttcccag gtcacgatgt aggggacctg gtctgggtgt ccatgcgggc
     5641 caggtgaaaa gaccttgatc ttaacctggg tgatgaggtc tcggttaaag gtgccgtctc
     5701 gcggccatcc gacgttaaag gttggccatt ctgcagagca gaaggtaacc caacgtctct
     5761 tcttgacatc taccgactgg ttgtgagcga tccgctcgac atctttccag tgacctaagg
     5821 tcaaacttaa gggagtggta acagtctggc cctaattttc agacaaatac agaaacacag
     5881 tcagacagag acaacacaga acgatgctgc agcgctgcag cagacaagac gcgcggcttc
     5941 ggttccaaac cgaaagcaaa aattcagacg gaggcgggaa ctgttttagg ttctcgtctc
     6001 ctaccagaac cacatatcct gacggggtcg gattccacat cgactccctt cctcaggtcg
     6061 ggccacaaaa acggccccca aagtccctgg gacgtctccc agggttgcgg ccgggtgttc
     6121 agaactcgtc agttccacca cgggtccgcc agatacagag ctagttagct aactagtacc
     6181 gacgcaggcg cataaaatca gtcatagaca ctagacaatc ggacagacac agataagttg
     6241 ctggccagct tgcctcccgg tggtgggtcg gtggtccctg ggcaggggtc tcccgatccc
     6301 ggacgagccc ccaaatgaaa gacccccgct gacgggtagt caatcactca gaggagaccc
     6361 tcccaaggaa cagcgagacc acaagtcgga tgcaactgca agagggttta ttggatacac
     6421 gggtacccgg gcgactcagt caatcggagg actggcgccc cgagtgaggg gttgtgggct
     6481 cttttattga gctcggggag cagaagcgcg cgaacagaag cgagaagcga actgattggt
     6541 tagttcaaat aaggcacagg gtcatttcag gtccttgggg caccctggaa acatctgatg
     6601 gttctctaga aactgctgag ggctggaccg catctgggga ccatctgttc ttggccctga
     6661 gccggggcag gaactgctta ccacagatat cctgtttggc ccatattcag ctgttccatc
     6721 tgttcttggc cctgagccgg ggcaggaact gcttaccaca gatatcctgt ttggcccata
     6781 ttcaggctgc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     6841 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     6901 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     6961 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     7021 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     7081 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     7141 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     7201 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     7261 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     7321 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     7381 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     7441 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     7501 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     7561 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     7621 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     7681 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     7741 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     7801 catatatact ttagattgat ttgcggccgc aaaaggatct aggtgaagat cctttttgat
     7861 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     7921 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     7981 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     8041 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     8101 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     8161 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     8221 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     8281 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     8341 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     8401 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     8461 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     8521 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     8581 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     8641 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     8701 gaagcggaag
//
