LOCUS Exported 807 bp ds-DNA linear SYN 20-MAY-2021 DEFINITION pRS416 Ura marked Cen/Ars plasmid with dCas9-Mxi1 under Tef1 promoter, and tet-incucibile RPR1 promoter with NotI cloning site adjacent to gRNA. ACCESSION . VERSION . KEYWORDS pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 807) AUTHORS Smith JD, Suresh S, Schlecht U, Wu M, Wagih O, Peltz G, Davis RW, Steinmetz LM, Parts L, St Onge RP TITLE Quantitative CRISPR interference screens in yeast identify chemical-genetic interactions and new rules for guide RNA design. JOURNAL Genome Biol. 2016 Mar 8;17(1):45. doi: 10.1186/s13059-016-0900-9. PUBMED 26956608 REFERENCE 2 (bases 1 to 807) AUTHORS . TITLE Direct Submission JOURNAL Exported May 20, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..807 /organism="synthetic DNA construct" /mol_type="other DNA" ORIGIN 1 atcactttac ttttatctaa tctagacatt ttaatatatt gtaatatgtg tgtttgtttg 61 gattattaag aagaataatt acaaaaaaaa ttacaaagga aggtaattac aacagaatta 121 agaaaggaca agaaggagga agagaatcag ttcattattt cttctttgtt atataacaaa 181 cccaagtagc gatttggcca tacattaaaa gttgagaacc accctccctg gcaacagcca 241 caactcgtta ccattgttca tcacgatcat gaaactcgct gtcagctgaa atttcacctc 301 agtggatctc tctttttatt cttcatcgtt ccactaacct ttttccatca gctggcaggg 361 aacggaaagt ggaatcccat ttagcgagct tcctcttttc ttcaagaaaa gacgaagctt 421 gtgtgtgggt gcgcgcgcta gtatctttcc acattaagaa atataccata aaggttactt 481 agacatcact atggctatac atatatatat atatgtaact tagcaccatc gcgcgtgcat 541 cactgcatgt gttaaccgaa aagtttggcg aacacttcac cgacacggtc atttagatct 601 gtcgtctgca ttgcacgtcc cttagcctta aatcctaggc gggagcattc tcgtgtaatt 661 gtgcagcctg cgtagcaact caacatagcg tagtctaccc agtttttcaa gggtttatcg 721 ttagaagatt ctcccttttc ttcctgctca caaatcttaa agtcatacat tgcacgacta 781 cctgcaggaa agaacaacaa gagttcc //