LOCUS Exported 816 bp ds-DNA linear SYN 17-MAY-2021 DEFINITION expresses mouse Piezo1 with GFP. ACCESSION . VERSION . KEYWORDS mPiezo1-IRES-eGFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 816) AUTHORS Coste B, Mathur J, Schmidt M, Earley TJ, Ranade S, Petrus MJ, Dubin AE, Patapoutian A TITLE Piezo1 and Piezo2 are essential components of distinct mechanically activated cation channels. JOURNAL Science. 2010 Oct 1;330(6000):55-60. doi: 10.1126/science.1193270. Epub 2010 Sep 2. PUBMED 20813920 REFERENCE 2 (bases 1 to 816) AUTHORS . TITLE Direct Submission JOURNAL Exported May 17, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..816 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(8..727) /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS complement(11..727) /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(53..74) /label=EGFP-C /note="EGFP, forward primer" primer_bind 402..421 /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 661..682 /label=EGFP-N /note="EGFP, reverse primer" ORIGIN 1 gccggcctta cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact 61 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactgggtgc 121 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt 181 agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct 241 tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact 301 ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc 361 ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt 421 ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt 481 cgtgctgctt catgtggtcg gggtagcggc tgaagcactg cacgccgtag gtcagggtgg 541 tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca 601 gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta 661 cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc 721 tcaccatggt tgtggccata ttatcatcgt gtttttcaaa ggaaaaccac gtccccgtgg 781 ttcggggggc ctagacgttt ttttaacctc gactaa //