LOCUS Exported 3266 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), Q61L mutation. ACCESSION . VERSION . KEYWORDS Hs.NRAS Q61L SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3266) AUTHORS Esposito TITLE RAS mutant collection V2.0 JOURNAL unpublished REFERENCE 2 (bases 1 to 3266) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3266 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 23..45 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 37..54 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 38..54 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 84..183 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(757..856) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" CDS 1233..2024 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 2117..2705 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2606..2625 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2859..2876 /label=L4440 /note="L4440 vector, forward primer" terminator 3035..3062 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 3154..3240 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" ORIGIN 1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa 61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt 121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg 181 gcaccatgac tgagtacaaa ctggtggtgg ttggagcagg tggtgttggg aaaagcgcac 241 tgacaatcca gctaatccag aaccactttg tagatgaata tgatcccacc atagaggatt 301 cttacagaaa acaagtggtt atagatggtg aaacctgttt gttggacata ctggatacag 361 ctggactaga agagtacagt gccatgagag accaatacat gaggacaggc gaaggcttcc 421 tctgtgtatt tgccatcaat aatagcaagt catttgcgga tattaacctc tacagggagc 481 agattaagcg agtaaaagac tcggatgatg tacctatggt gctagtggga aacaagtgtg 541 atttgccaac aaggacagtt gatacaaaac aagcccacga actggccaag agttacggga 601 ttccattcat tgaaacctca gccaagacca gacagggtgt tgaagatgct ttttacacac 661 tggtaagaga aatacgccag taccgaatga aaaaactcaa cagcagtgat gatgggactc 721 agggttgtat gggattgcca tgtgtggtga tgtaataatc aactttcttg tacaaagttg 781 gcattatagg aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa 841 ataaaatcat tatttgcatg caagcttaga tcctctagac cagccaggac agaaatgcct 901 cgacttcgct gctacccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac 961 gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct 1021 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt 1081 tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc 1141 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc 1201 agggcagtcg ccctaaaaca aagttaaaca ttatgaggga agcggtgatc gccgaagtat 1261 cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg 1321 ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt 1381 tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc 1441 ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca 1501 ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg 1561 gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg 1621 atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg 1681 cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct 1741 taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt 1801 tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg 1861 actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg 1921 cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg 1981 tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg agccacccat 2041 gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa 2101 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 2161 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 2221 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 2281 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 2341 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 2401 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 2461 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg 2521 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 2581 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 2641 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 2701 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 2761 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 2821 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 2881 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca 2941 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatacgc 3001 gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt 3061 ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg 3121 ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac 3181 cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc gttttatttg 3241 atgcctggca gttccctact ctcgcg //