LOCUS Exported 3260 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway ORF Entry clone of human HRAS [NM_005343.2 ] with stop codon (for native or N-terminal fusions). ACCESSION . VERSION . KEYWORDS Hs.HRAS SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3260) AUTHORS Esposito TITLE RAS mutant collection V2.0 JOURNAL unpublished REFERENCE 2 (bases 1 to 3260) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3260 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 23..45 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 37..54 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 38..54 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 84..183 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" CDS 183..752 /codon_start=1 /product="human small GTPase proto-oncoprotein" /label=H-Ras /translation="MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVK DSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREI RQHKLRKLNPPDESGPGCMSCKCVLS" protein_bind complement(751..850) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" CDS 1227..2018 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 2111..2699 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2600..2619 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2853..2870 /label=L4440 /note="L4440 vector, forward primer" terminator 3029..3056 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 3148..3234 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" ORIGIN 1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa 61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt 121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg 181 gcatgacgga atataagctg gtggtggtgg gcgccggcgg tgtgggcaag agtgcgctga 241 ccatccagct gatccagaac cattttgtgg acgaatacga ccccactata gaggattcct 301 accggaagca ggtggtcatt gatggggaga cgtgcctgtt ggacatcctg gataccgccg 361 gccaggagga gtacagcgcc atgcgggacc agtacatgcg caccggggag ggcttcctgt 421 gtgtgtttgc catcaacaac accaagtctt ttgaggacat ccaccagtac agggagcaga 481 tcaaacgggt gaaggactcg gatgacgtgc ccatggtgct ggtggggaac aagtgtgacc 541 tggctgcacg cactgtggaa tctcggcagg ctcaggacct cgcccgaagc tacggcatcc 601 cctacatcga gacctcggcc aagacccggc agggagtgga ggatgccttc tacacgttgg 661 tgcgtgagat ccggcagcac aagctgcgga agctgaaccc tcctgatgag agtggccccg 721 gctgcatgag ctgcaagtgt gtgctctcct aatcaacttt cttgtacaaa gttggcatta 781 taggaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa 841 tcattatttg catgcaagct tagatcctct agaccagcca ggacagaaat gcctcgactt 901 cgctgctacc caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc 961 agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca 1021 actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg 1081 cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc 1141 gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca 1201 gtcgccctaa aacaaagtta aacattatga gggaagcggt gatcgccgaa gtatcgactc 1261 aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac 1321 atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg 1381 ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg 1441 aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg 1501 tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat 1561 ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg 1621 ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg 1681 aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc 1741 tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc 1801 gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg 1861 caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc 1921 ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact 1981 acgtgaaagg cgagatcacc aaggtagtcg gcaaataacc ctcgagccac ccatgaccaa 2041 aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca 2101 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac 2161 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg 2221 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag 2281 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac 2341 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt 2401 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg 2461 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc 2521 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc 2581 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc 2641 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa 2701 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt 2761 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg 2821 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag 2881 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 2941 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg 3001 ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt 3061 agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt 3121 cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa 3181 acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct 3241 ggcagttccc tactctcgcg //