LOCUS Exported 879 bp ds-DNA linear SYN 29-MAY-2021 DEFINITION CRISPaint gene tagging. ACCESSION . VERSION . KEYWORDS pCRISPaint-cTEV-PuroR SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 879) AUTHORS Schmid-Burgk JL, Honing K, Ebert TS, Hornung V TITLE CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism. JOURNAL Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338. PUBMED 27465542 REFERENCE 2 (bases 1 to 879) AUTHORS . TITLE Direct Submission JOURNAL Exported May 29, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..879 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(612..665) /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" ORIGIN 1 gaagcgggcc ctcacgctcc aggcttcctc gtcatgcacc aagtgcgggg gccttcgggg 61 acttccacgt cggctgtcac ggtgaagccc aaccgttcat agaagggaag attccttgga 121 gcggaggtct ccaagaaggc aggtactcct gctctctcag ctgcctccac gccagggagc 181 actacggcag agcccagacc tttcccctga tgatcgggag atacaccgac ggttgccagg 241 aaccaggcgg gttctttggg cctgtgtggt gccagcagtc cctccatttg ctgctgtgcc 301 gcgagcctac tcccgctgag ctcagccatt ctcgggccga tctcagcgaa tacggcgcca 361 gcctcaactg attcgggagt ggtccagacg gccacggcgg cgccgtcgtc cgccacccac 421 accttgccta tgtcgaggcc aactcttgta agaaacagct cctgcagttc agtcactctc 481 tcaatatgcc gatctgggtc aacggtgtgt ctcgtggcgg ggtagtcggc gaaggctgca 541 gccagagttc tcacggctct aggaacgtcg tctcttgtag ccaacctgac agtaggcttg 601 tattcagtca ttgggccagg attctcctcg acgtcaccgc atgttagcag acttcctctg 661 ccctctcctc cggactggct gtacaccagc tcgttcatca gctgggtggc ctccttcacg 721 ggctggaagg gctcctcggg cttgctcatg aacaccttgt ggccgcccca cagcacgctg 781 tcggcgttca gtctccagcc gctcacccac tgctgggcct cctggttggt cagcagctcc 841 atgaagttct tgggcacgct ggtgaagtag ttgttggtg //