LOCUS Exported 781 bp ds-DNA linear SYN 25-MAY-2021 DEFINITION Cas9, mCherry, and sgRNA expression. ACCESSION . VERSION . KEYWORDS pCAS9-mCherry empty SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 781) AUTHORS Schmid-Burgk JL, Honing K, Ebert TS, Hornung V TITLE CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism. JOURNAL Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338. PUBMED 27465542 REFERENCE 2 (bases 1 to 781) AUTHORS . TITLE Direct Submission JOURNAL Exported May 25, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..781 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 264..317 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" ORIGIN 1 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc 61 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg 121 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg 181 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagg 241 tcgctaccat cgagggatcc ggagagggca gaggaagtct gctaacatgc ggtgacgtcg 301 aggagaatcc tggcccaatg gacaagaagt acagcatcgg cctggacatc ggcaccaaca 361 gcgtgggctg ggccgtgatc accgacgagt acaaggtgcc cagcaagaag ttcaaggtgc 421 tgggcaacac cgaccgccac agcatcaaga agaacctgat cggcgccctg ctgttcgaca 481 gcggcgagac cgccgaggcc acccgcctga agcgcaccgc ccgccgccgc tacacccgcc 541 gcaagaaccg catctgctac ctgcaggaga tcttcagcaa cgagatggcc aaggtggacg 601 acagcttctt ccaccgcctg gaggagagct tcctggtgga ggaggacaag aagcacgagc 661 gccaccccat cttcggcaac atcgtggacg aggtggccta ccacgagaag taccccacca 721 tctaccacct gcgcaagaag ctggtggaca gcaccgacaa ggccgacctg cgcctgatct 781 a //