LOCUS Exported 10949 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Lenti-mediated expression of Chronos-GFP under the CaMKII promoter.. ACCESSION . VERSION . KEYWORDS FCK-Chronos-GFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10949) AUTHORS Klapoetke NC, Murata Y, Kim SS, Pulver SR, Birdsey-Benson A, Cho YK, Morimoto TK, Chuong AS, Carpenter EJ, Tian Z, Wang J, Xie Y, Yan Z, Zhang Y, Chow BY, Surek B, Melkonian M, Jayaraman V, Constantine-Paton M, Wong GK, Boyden ES TITLE Independent optical excitation of distinct neural populations. JOURNAL Nat Methods. 2014 Mar;11(3):338-46. doi: 10.1038/nmeth.2836. Epub 2014 Feb 9. PUBMED 24509633 REFERENCE 2 (bases 1 to 10949) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10949 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(47..66) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 238..617 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 619..817 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 772..792 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 835..1015 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1062..1187 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1680..1913 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2098..2142 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2440..2557 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2602..3891 /label=CaMKII promoter /note="promoter for the mouse calcium/calmodulin-dependent kinase II alpha subunit" regulatory 3901..3910 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 4900..5613 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" primer_bind complement(4942..4963) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(5203..5222) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 5550..5571 /label=EGFP-C /note="EGFP, forward primer" misc_feature 5641..6229 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5694..5714) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6112..6123) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(6232..6248) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6233..6249) /label=pBluescriptKS /note="For pBluescript vector" LTR 6754..6934 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(6960..6977) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 6966..7190 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 7236..7664 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7323..7342) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7533..7554 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(7673..7693) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 7678..8007 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 7858..7993 /label=SV40 ori /note="SV40 origin of replication" primer_bind 7920..7939 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 8055..8102 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 8121..8495 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 8625..8746 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8662..8681) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8716..8735 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(8795..8811) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8795..8811) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8808..8830) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8819..8835 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8843..8873) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8888..8909 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(9026..9043) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(9197..9785) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(9277..9296) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9956..10816) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 10579..10598 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10817..10921) /gene="bla" /label=AmpR promoter ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggttaatta acattatggc cttaggtcac ttcatctcca tggggttctt 2641 cttctgattt tctagaaaat gagatggggg tgcagagagc ttcctcagtg acctgcccag 2701 ggtcacatca gaaatgtcag agctagaact tgaactcaga ttactaatct taaattccat 2761 gccttggggg catgcaagta cgatatacag aaggagtgaa ctcattaggg cagatgacca 2821 atgagtttag gaaagaagag tccagggcag ggtacatcta caccacccgc ccagccctgg 2881 gtgagtccag ccacgttcac ctcattatag ttgcctctct ccagtcctac cttgacggga 2941 agcacaagca gaaactggga caggagcccc aggagaccaa atcttcatgg tccctctggg 3001 aggatgggtg gggagagctg tggcagaggc ctcaggaggg gccctgctgc tcagtggtga 3061 cagatagggg tgagaaagca gacagagtca ttccgtcagc attctgggtc tgtttggtac 3121 ttcttctcac gctaaggtgg cggtgtgata tgcacaatgg ctaaaaagca gggagagctg 3181 gaaagaaaca aggacagaga cagaggccaa gtcaaccaga ccaattccca gaggaagcaa 3241 agaaaccatt acagagacta caagggggaa gggaaggaga gatgaattag cttcccctgt 3301 aaaccttaga acccagctgt tgccagggca acggggcaat acctgtctct tcagaggaga 3361 tgaagttgcc agggtaacta catcctgtct ttctcaagga ccatcccaga atgtggcacc 3421 cactagccgt taccatagca actgcctctt tgccccactt aatcccatcc cgtctgttaa 3481 aagggcccta tagttggagg tgggggaggt aggaagagcg atgatcactt gtggactaag 3541 tttgttcaca tccccttctc caaccccctc agtacatcac cctgggagaa caaggtccac 3601 ttgcttctgg gcccacacag tcctgcagta ttgtgtatat aaggccaggg caacggagga 3661 gcaggttttg aagtgaaagg caggcaggtg ttggggaggc agttaccggg gcaacgggaa 3721 cagggcgttt cggaggtggt tgccatgggg acctggatgc tgacgaaggc tcgcgaggct 3781 gtgagcagcc acagtgccct gctcagaagc cccaagctcg tcaatcaagc tggttctcca 3841 tttgcactca ggagcacggg caggcgagtg gcccctagtt ctgggggcag cgggggatcc 3901 gccaccatgg aaacagccgc cacaatgacc cacgccttta tctcagccgt gcctagcgcc 3961 gaagccacaa ttagaggcct gctgagcgcc gcagcagtgg tgacaccagc agcagacgct 4021 cacggagaaa cctctaacgc cacaacagcc ggagccgatc acggttgctt cccccacatc 4081 aaccacggaa ccgagctgca gcacaagatc gcagtgggac tccagtggtt caccgtgatc 4141 gtggctatcg tgcagctcat cttctacggt tggcacagct tcaaggccac aaccggctgg 4201 gaggaggtct acgtctgcgt gatcgagctc gtcaagtgct tcatcgagct gttccacgag 4261 gtcgacagcc cagccacagt gtaccagacc aacggaggag ccgtgatttg gctgcggtac 4321 agcatgtggc tcctgacttg ccccgtgatc ctgatccacc tgagcaacct gaccggactg 4381 cacgaagagt acagcaagcg gaccatgacc atcctggtga ccgacatcgg caacatcgtg 4441 tgggggatca cagccgcctt tacaaagggc cccctgaaga tcctgttctt catgatcggc 4501 ctgttctacg gcgtgacttg cttcttccag atcgccaagg tgtatatcga gagctaccac 4561 accctgccca aaggcgtctg ccggaagatt tgcaagatca tggcctacgt cttcttctgc 4621 tcttggctga tgttccccgt gatgttcatc gccggacacg agggactggg cctgatcaca 4681 ccttacacca gcggaatcgg ccacctgatc ctggatctga tcagcaagaa cacttggggc 4741 ttcctgggcc accacctgag agtgaagatc cacgagcaca tcctgatcca cggcgacatc 4801 cggaagacaa ccaccatcaa cgtggccggc gagaacatgg agatcgagac cttcgtcgac 4861 gaggaggagg agggaggagt ggcggcaccg gtagtagcag tgagcaaggg cgaggagctg 4921 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc 4981 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatt 5041 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc 5101 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc 5161 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag 5221 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc 5281 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc 5341 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc 5401 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc 5461 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg 5521 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc 5581 gggatcactc tcggcatgga cgagctgtac aagtaagaat tcgatatcaa gcttatcgat 5641 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct 5701 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt 5761 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg 5821 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact 5881 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct 5941 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg 6001 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc 6061 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc 6121 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt 6181 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt 6241 cgacctcgag acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat 6301 gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct 6361 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa 6421 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg 6481 tggatctacc acacacaagg ctacttccct gattggcaga actacacacc agggccaggg 6541 atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag 6601 gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg 6661 atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat 6721 cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga 6781 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 6841 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 6901 agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc 6961 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt 7021 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca 7081 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga 7141 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc 7201 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag 7261 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc 7321 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc 7381 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa 7441 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg 7501 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac 7561 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta 7621 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg 7681 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 7741 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt 7801 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc 7861 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt 7921 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc 7981 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga 8041 tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg 8101 acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc 8161 gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg 8221 tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc 8281 aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc 8341 tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca 8401 tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca 8461 actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca 8521 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga 8581 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag 8641 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt 8701 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac 8761 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt 8821 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 8881 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 8941 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 9001 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 9061 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 9121 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 9181 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 9241 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 9301 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 9361 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 9421 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 9481 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 9541 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 9601 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc 9661 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 9721 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 9781 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 9841 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 9901 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 9961 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 10021 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 10081 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 10141 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 10201 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 10261 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 10321 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 10381 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 10441 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 10501 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 10561 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 10621 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 10681 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 10741 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 10801 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 10861 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 10921 gcacatttcc ccgaaaagtg ccacctgac //