LOCUS       Exported                 912 bp ds-DNA     linear   SYN 19-MAY-2021
DEFINITION  Expresses FLAG-tagged ZnT1-WT in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    FLAG-ZnT1-WT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Zogzas CE, Aschner M, Mukhopadhyay S
  TITLE     Structural Elements in the Transmembrane and Cytoplasmic Domains of 
            the Metal Transporter SLC30A10 Are Required for Its Manganese Efflux
            Activity.
  JOURNAL   J Biol Chem. 2016 Jul 29;291(31):15940-57. doi: 
            10.1074/jbc.M116.726935. Epub 2016 Jun 15.
  PUBMED    27307044
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..912
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             46..111
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagaattaa ccatggacta caaagaccat
       61 gacggtgatt ataaagatca tgacatcgat tacaaggatg acgatgacaa gcttgcggcc
      121 gcgatggggt gttggggtcg gaaccggggc cggctgctgt gcatgctggc gctgaccttc
      181 atgttcatgg tgctggaggt ggtggtgagc cgggtgacct cgtcgctggc gatgctctcc
      241 gactccttcc acatgctgtc ggacgtgctg gcgctggtgg tggcgctggt ggccgagcgc
      301 ttcgcccggc ggacccacgc cacccagaag aacacgttcg gctggatccg agccgaggta
      361 atgggggctc tggtgaacgc catcttcctg actggcctct gtttcgccat cctgctggag
      421 gccatcgagc gcttcatcga gccgcacgag atgcagcagc cgctggtggt ccttggggtc
      481 ggcgtggccg ggctgctggt caacgtgctg gggctctgcc tcttccacca tcacagcggc
      541 ttcagccagg actccggcca cggccactcg cacgggggtc acggccacgg ccacggcctc
      601 cccaaggggc ctcgcgttaa gagcacccgc cccgggagca gcgacatcaa cgtggccccg
      661 ggcgagcagg gtcccgacca ggaggagacc aacaccctgg tggccaatac cagcaactcc
      721 aacgggctga aattggaccc cgcagaccca gaaaacccca gaagtggtga tacagtggaa
      781 gtacaagtga atggaaatct tgtcagagaa cctgaccata tggaactgga agaagatagg
      841 gctggacaac ttaacatgcg tggagttttt ctgcatgtcc ttggagatgc cttgggttca
      901 gtgattgtag ta
//
