LOCUS       Exported                 452 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  3rd generation lentiviral plasmid for inducible expression of shRNA;
            neomycin selection. See manual for detailed protocols. No further 
            technical support is available from the depositing laboratory..
ACCESSION   .
VERSION     .
KEYWORDS    Tet-pLKO-neo
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Wiederschain D, Wee S, Chen L, Loo A, Yang G, Huang A, Chen Y, 
            Caponigro G, Yao YM, Lengauer C, Sellers WR, Benson JD
  TITLE     Single-vector inducible lentiviral RNAi system for oncology target 
            validation.
  JOURNAL   Cell Cycle. 2009 Feb 1. 8(3):498-504.
  PUBMED    19177017
REFERENCE   2  (bases 1 to 452)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    8..26
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 tataagttcc ctatcagtga tagagacacc ggtgtgttgt aaatgagcac acaaaataca
       61 catgctaaaa tattatattc tatgaccttt ataaaatcaa ccaaaatctt ctttttaata
      121 actttagtat caataattag aatttttatg ttcctttttg caaactttta ataaaaatga
      181 gcaaaataaa aaaacgctag ttttagtaac tcgcgttgtt ttcttcacct ttaataatag
      241 ctactccacc acttgttcct aagcggtcag ctcctgcttc aatcattttt tgagcatctt
      301 caaatgttct aactccacca gctgctttaa ctaaagcatt gtctttaaca actgacttca
      361 ttagtttaac atcttcaaat gttgcacctg attttgaaaa tcctgttgat gttttaacaa
      421 attctaatcc agcttcaaca gctatttcac aa
//
