LOCUS Exported 859 bp ds-DNA linear SYN 17-MAY-2021 DEFINITION expresses mouse Piezo1 with GFP. ACCESSION . VERSION . KEYWORDS mPiezo1-IRES-eGFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 859) AUTHORS Coste B, Mathur J, Schmidt M, Earley TJ, Ranade S, Petrus MJ, Dubin AE, Patapoutian A TITLE Piezo1 and Piezo2 are essential components of distinct mechanically activated cation channels. JOURNAL Science. 2010 Oct 1;330(6000):55-60. doi: 10.1126/science.1193270. Epub 2010 Sep 2. PUBMED 20813920 REFERENCE 2 (bases 1 to 859) AUTHORS . TITLE Direct Submission JOURNAL Exported May 17, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..859 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 49..68 /label=T7 /note="T7 promoter, forward primer" promoter 49..67 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 120..129 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" ORIGIN 1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca 61 ctatagggag acccaagctg gctagcgttt aaacgggccc tctagactcg agcggccgcg 121 ccaccatgga gccgcacgtg ctgggcgccg ggctctactg gctgttgctg ccctgcacgc 181 tcctggcggc ctccctgtta cgcttcaatg ctctctcgct ggtctatttg ctgtttctac 241 tgctgctgcc ctggcttcca ggcccctcaa gacacagcat accaggtcac acaggtcgcc 301 tgctccgtgc actactctgc ctcagcctcc tcttcctggt ggcccacctt gcctttcaga 361 tatgcctaca caccgtgcct cacctggacc agtttctggg acaaaacggt agcctttggg 421 tgaaggtgtc tcaacacata ggggttacga ggctggacct gaaggacatc tttaacacca 481 ccaggctggt agcacctgac ctgggagtgc tgctggcgtc ctccctttgc cttggcctct 541 gtggacgcct cacgaggaaa gccgggcaga gtcggcgcac ccaggagctg caggatgatg 601 acgatgacga tgatgacgac gatgaagaca tagatgctgc cccagccgtg gggctgaagg 661 gagcccctgc cctggcaacc aaacgcaggc tgtggctggc ctcccgcttc cgggtcacgg 721 cccactggct gctgatgacc tctggacgga cgctggtcat tgtgttgctg gccctggcag 781 gcatagccca cccttcggcc ttctccagca tctacctggt ggtgttcctg gccatctgca 841 cctggtggtc ctgtcactt //