LOCUS Exported 986 bp ds-DNA linear SYN 29-MAY-2021 DEFINITION This plasmid encodes the kinase domain of MARK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. . ACCESSION . VERSION . KEYWORDS MARK3_HUMAN_D0 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 986) AUTHORS Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD TITLE An open library of human kinase domain constructs for automated bacterial expression. JOURNAL Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081. PUBMED 30004690 REFERENCE 2 (bases 1 to 986) AUTHORS . TITLE Direct Submission JOURNAL Exported May 29, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..986 /organism="synthetic DNA construct" /mol_type="other DNA" RBS 61..83 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" CDS 103..132 /codon_start=1 /product="10xHis affinity tag" /label=10xHis /translation="HHHHHHHHHH" CDS 133..153 /codon_start=1 /product="tobacco etch virus (TEV) protease recognition and cleavage site" /label=TEV site /translation="ENLYFQS" ORIGIN 1 ggctccggag agctctttaa ttaagcggcc gccctgcagg actcgagttc tagaaataat 61 tttgtttaac tttaagaagg agatatacat atgaaatctt ctcaccatca ccatcaccat 121 caccatcacc atgaaaacct gtacttccaa tccaatgcag atgaacaacc tcacatcgga 181 aactacagac tgttgaaaac aatcggcaag gggaattttg caaaagtaaa attggcaaga 241 catatcctta caggcagaga ggttgcaata aaaataattg acaaaactca gttgaatcca 301 acaagtctac aaaagctctt cagagaagta agaataatga agattttaaa tcatcccaat 361 atagtgaagt tattcgaagt cattgaaact gaaaaaacac tctacctaat catggaatat 421 gcaagtggag gtgaagtatt tgactatttg gttgcacatg gcaggatgaa ggaaaaagaa 481 gcaagatcta aatttagaca gattgtgtct gcagttcaat actgccatca gaaacggatc 541 gtacatcgag acctcaaggc tgaaaatcta ttgttagatg ccgatatgaa cattaaaata 601 gcagatttcg gttttagcaa tgaatttact gttggcggta aactcgacac gttttgtggc 661 agtcctccat acgcagcacc tgagctcttc cagggcaaga aatatgacgg gccagaagtg 721 gatgtgtgga gtctgggggt cattttatac acactagtca gtggctcact tccctttgat 781 gggcaaaacc taaaggaact gagagagaga gtattaagag ggaaatacag aattcccttc 841 tacatgtcta cagactgtga aaaccttctc aaacgtttcc tggtgctaaa tccaattaaa 901 cgcggcactc tagagcaaat catgaaggac aggtggatca atgcagggca tgaagaagat 961 gaactcaaac catttgttga accaga //