LOCUS       Exported                 835 bp ds-DNA     linear   SYN 23-MAY-2021
DEFINITION  Expression of redox sensitive GFP with a mitochondrial targeting 
            sequence in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pEGFP-N1-mt-ro1GFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Hanson GT, Aggeler R, Oglesbee D, Cannon M, Capaldi RA, Tsien RY, 
            Remington SJ
  TITLE     Investigating mitochondrial redox potential with redox-sensitive 
            green fluorescent protein indicators.
  JOURNAL   J Biol Chem. 2004 Mar 26;279(13):13044-53. Epub 2004 Jan 13.
  PUBMED    14722062
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 23, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      274..283
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             280..798
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFISTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNCHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(325..346)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(586..605)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tcagatccgc tagcgctacc ggactcagat ctcgagctcg aattcgggct gaaggagact
       61 tgggggcacc cgcgtcgtgc ctcctgggtt gtgaggagtc gccgctgccg ccactgcctg
      121 tgcttcatga ggaagatgct cgccgccgtc tcccgcgtgc tgtctggcgc ttctcagaag
      181 ccggcaagca gagtgctggt agcatcccgt aattttgcaa atgatgctac atttctgcag
      241 tcgacggtac cgcgggcccg ggatccaccg gtcgccacca tggtgagcaa gggcgaggag
      301 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
      361 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
      421 atctccacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgtcctac
      481 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
      541 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
      601 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
      661 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
      721 tgccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
      781 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcaga
//
