LOCUS Exported 9093 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION 3rd-generation lentiviral destination vector for gateway cloning with puromycin resistance.. ACCESSION . VERSION . KEYWORDS pEpic SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9093) AUTHORS Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne P TITLE A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate Expression Constructs with Diverse Research Applications. JOURNAL PLoS One. 2016 Aug 8;11(8):e0159277. doi: 10.1371/journal.pone.0159277. eCollection 2016. PUBMED 27500400 REFERENCE 2 (bases 1 to 9093) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9093 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 27..131 /gene="bla" /label=AmpR promoter CDS 132..992 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(350..369) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1163..1751 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1652..1671 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 1905..1922 /label=L4440 /note="L4440 vector, forward primer" protein_bind 2039..2060 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 2075..2105 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2113..2129 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2118..2140 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 2137..2153 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 2172..2192 /label=T3 /note="T3 promoter, forward primer" promoter 2174..2192 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" LTR 2448..2628 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 2672..2797 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 3290..3523 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 3708..3752 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 4019..4136 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" primer_bind 4175..4191 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind 4210..4334 /gene="mutant version of attR" /label=attR4 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" CDS complement(4375..4680) /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" primer_bind complement(4477..4496) /label=ccdB-fwd /note="ccdB gene, forward primer" CDS complement(5022..5681) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 5596..5615 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" promoter complement(5735..5765) /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" protein_bind complement(5791..5914) /gene="mutant version of attR" /label=attR3 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(5922..5938) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" misc_feature 5975..6563 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6028..6048) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6446..6457) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" promoter 6577..7076 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" primer_bind complement(6612..6635) /label=MSCV-rev /note="Murine stem cell virus, reverse primer" primer_bind 7009..7028 /label=mPGK-F /note="Mouse PGK promoter, forward primer" CDS 7097..7696 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(7097..7116) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 7593..7613 /label=Puro-F /note="Puromycin resistance gene, forward primer" LTR 7834..8067 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 8139..8260 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8176..8195) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8230..8249 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 8279..8414 /label=SV40 ori /note="SV40 origin of replication" primer_bind 8341..8360 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(8446..8465) /label=T7 /note="T7 promoter, forward primer" promoter complement(8447..8465) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(8475..8492) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(8475..8491) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8484..8506) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 8636..9091 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(8723..8742) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 8933..8954 /label=F1ori-F /note="F1 origin, forward primer" ORIGIN 1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta 2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt 2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat 2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc 2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg 2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc 2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt 2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa 2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga 2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag 2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag 2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca 2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac 2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga 3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga 3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac 3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt 3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca 3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt 3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg 3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta 3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa 3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct 3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc 3601 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca 3661 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag 3721 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc 3781 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt 3841 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga 3901 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag 3961 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt 4021 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag 4081 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg 4141 atcacgagac tagcctcgag aagcttgatt cgagcaggaa acagctatga ccatgattac 4201 gccaagctat caactttgta tagaaaagtt gaacgagaaa cgtaaaatga tataaatatc 4261 aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat 4321 atccagtcac tatggtcgac ctgcagactg gctgtgtata agggagcctg acatttatat 4381 tccccagaac atcaggttaa tggcgttttt gatgtcattt tcgcggtggc tgagatcagc 4441 cacttcttcc ccgataacgg agaccggcac actggccata tcggtggtca tcatgcgcca 4501 gctttcatcc ccgatatgca ccaccgggta aagttcacgg gggactttat ctgacagcag 4561 acgtgcactg gccaggggga tcaccatccg tcgcccgggc gtgtcaataa tatcactctg 4621 tacatccaca aacagacgat aacggctctc tcttttatag gtgtaaacct taaactgcat 4681 ttcaccagcc cctgttctcg tcggcaaagg agccgttcat ttcaataaac cgggcgacct 4741 cagccatccc ttcctgattt tccgctttcc agcgttcggc acgcagacga cgggcttcat 4801 tctgcatggt tgtgcttacc gaaccggaga tattgacatc atatatgcct tgagcaactg 4861 atagctgtcg ctgtcaactg tcactgtaat acgctgcttc atagcatacc tctttttgac 4921 atacttcggg tatacatatc agtatatatt cttataccgc aaaaatcagc gcgcaaatac 4981 gcatactgtt atctggcttt tagtaagccg gatcctctag attacgcccc gccctgccac 5041 tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg 5101 gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg 5161 cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg 5221 gtgaaactca cccagggatt ggctgagacg aaaaacatat tctcaataaa ccctttaggg 5281 aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc 5341 cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa 5401 acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata 5461 cggaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac 5521 ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg 5581 ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg 5641 gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct 5701 gaaaatctcg acggatccta actcaaaatc cacacattat acgagccgga agcataaagt 5761 gtaaagcctg ggggtgccta atgcggccgc catagtgact ggatatgttg tgttttacag 5821 tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt 5881 tacgtttctc gttcaacttt attatacata gttgataatt cactggccgt cgttttacgg 5941 taccatcgat ccagcacagt ggcggccgct cgacaatcaa cctctggatt acaaaatttg 6001 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc 6061 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta 6121 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt 6181 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca 6241 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc 6301 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt 6361 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg 6421 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg 6481 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat 6541 ctccctttgg gccgcctccc cgcctggaat tctaccgggt aggggaggcg cttttcccaa 6601 ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc 6661 tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg 6721 gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc 6781 tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg 6841 acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt 6901 gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg 6961 gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc 7021 ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct 7081 gcagcccaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga 7141 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca 7201 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac 7261 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt 7321 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat 7381 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc 7441 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca 7501 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg 7561 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg 7621 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg 7681 caagcccggt gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg 7741 accccatgca tctcgagggc ccggtacctt taagaccaat gacttacaag gcagctgtag 7801 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctagctcac tcccaacgaa 7861 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg 7921 agctctctgg ctgcctaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc 7981 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct 8041 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt 8101 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata 8161 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 8221 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat 8281 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt 8341 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg 8401 cttttttgga ggcctaggct tttgcgtcga gacgtaccca attcgcccta tagtgagtcg 8461 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt 8521 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag 8581 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcgacgcg 8641 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca 8701 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc 8761 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct 8821 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg 8881 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc 8941 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg 9001 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg 9061 aattttaaca aaatattaac gtttacaatt tcc //