LOCUS       Exported                 852 bp ds-DNA     linear   SYN 24-MAY-2021
DEFINITION  Cre dependent expression of GCaMP6f Ca sensor and physically 
            separate nuclear localized dTomato fluorophore .
ACCESSION   .
VERSION     .
KEYWORDS    AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 852)
  TITLE     Ting GCaMP6 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 24, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(90..111)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(351..370)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ctctcggcat ggacgagctg tacaagggcg gtaccggagg gagcatggtg agcaagggcg
       61 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
      121 acaagttcag cgtgtccggc gagggtgagg gcgatgccac ctacggcaag ctgaccctga
      181 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
      241 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
      301 agtccgccat gcccgaaggc tacatccagg agcgcaccat cttcttcaag gacgacggca
      361 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
      421 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaacc
      481 tgccggacca actgactgaa gagcagatcg cagaatttaa agaggaattc tccctatttg
      541 acaaggacgg ggatgggaca ataacaacca aggagctggg gacggtgatg cggtctctgg
      601 ggcagaaccc cacagaagca gagctgcagg acatgatcaa tgaagtagat gccgacggtg
      661 acggcacaat cgacttccct gagttcctga caatgatggc aagaaaaatg aaatacaggg
      721 acacggaaga agaaattaga gaagcgttcg gtgtgtttga taaggatggc aatggctaca
      781 tcagtgcagc agagcttcgc cacgtgatga caaaccttgg agagaagtta acagatgaag
      841 aggttgatga aa
//
