LOCUS       Exported                 777 bp ds-DNA     linear   SYN 24-MAY-2021
DEFINITION  Cre dependent expression of GCaMP6f Ca sensor and physically 
            separate nuclear localized dTomato fluorophore .
ACCESSION   .
VERSION     .
KEYWORDS    AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 777)
  TITLE     Ting GCaMP6 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 24, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    48..81
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites (Lee and 
                     Saito, 1988)."
     protein_bind    132..165
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     primer_bind     complement(266..289)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(464..483)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     733..751
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 catttcaggt gtcgtgaggt accggatcct ctagagtcga ctccggaata acttcgtata
       61 ggatacttta tacgaagtta tgcagaatgg tagctggatt gtagctgcta ttagcaatat
      121 gaaacctctt aataacttcg tatagcatac attatacgaa gttatggcgc gccgtcgact
      181 tacttgtaca gctcgtccat gccgtacagg aacaggtggt ggcggccctc ggagcgctcg
      241 tactgttcca cgatggtgta gtcctcgttg tgggaggtga tgtccagctt ggtgtccacg
      301 tagtagtagc cgggcagttg cacgggcttc ttggccatgt agatggtctt gaactccacc
      361 aggtagtggc cgccgtcctt cagcttcagg gcctggtgga tctcgccctt cagcacgccg
      421 tcgcgggggt acaggcgctc ggtggaggcc tcccagccca tggtcttctt ctgcattacg
      481 gggccgtcgg gggggaagtt ggtgccgcgc atcttcacct tgtagatcag cgtgccgtcc
      541 tgcagggagg agtcctgggt cacggtcacc agaccgccgt cctcgaagtt catcacgcgc
      601 tcccacttga agccctcggg gaaggacagc ttcttgtaat cggggatgtc ggcggggtgc
      661 ttcacgtacg ccttggagcc gtacatgaac tggggggaca ggatgtccca ggcgaagggc
      721 agggggccgc ccttggtcac cttcagcttg gcggtctggg tgccctcgta ggggcgg
//
