LOCUS       Exported                 898 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  Constitutive expression of CyOFP in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    pNCS-CyOFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Chu J, Oh Y, Sens A, Ataie N, Dana H, Macklin JJ, Laviv T, Welf ES, 
            Dean KM, Zhang F, Kim BB, Tang CT, Hu M, Baird MA, Davidson MW, Kay 
            MA, Fiolka R, Yasuda R, Kim DS, Ng HL, Lin MZ
  TITLE     A bright cyan-excitable orange fluorescent protein facilitates 
            dual-emission microscopy and enhances bioluminescence imaging in 
            vivo.
  JOURNAL   Nat Biotechnol. 2016 May 30. doi: 10.1038/nbt.3550.
  PUBMED    27240196
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     RBS             46..68
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             88..105
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             109..141
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             145..168
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             175..876
                     /codon_start=1
                     /product="orange-red fluorescent protein that is excitable 
                     by cyan light and suitable as an acceptor for blue-emitting
                     luciferase (Chu et al., 2016)"
                     /label=CyOFP1
                     /translation="MVSKGEELIKENMRSKLYLEGSVNGHQFKCTHEGEGKPYEGKQTN
                     RIKVVEGGPLPFAFDILATHFMYGSKVFIKYPADLPDYFKQSFPEGFTWERVMVFEDGG
                     VLTATQDTSLQDGELIYNVKVRGVNFPANGPVMQKKTLGWEPSTETMYPADGGLEGRCD
                     KALKLVGGGHLHVNFKTTYKSKKPVKMPGVHYVDRRLERIKEADNETYVEQYEHAVARY
                     SNLGGGMDELYK"
ORIGIN
        1 ttcttgacac ctgatgcgat gaatgatata ataggaaagt actgttttgt ttaactttaa
       61 gaaggagata tacatatgcg gggttctcat catcatcatc atcatggtat ggctagcatg
      121 actggtggac agcaaatggg tcgggatctg tacgacgatg acgataagga tccgatggtg
      181 agcaagggcg aggagctgat caaggagaac atgagaagca agctgtacct ggaaggcagc
      241 gtgaacggcc accagttcaa gtgcacccac gaaggggagg gcaagcccta cgagggcaag
      301 cagaccaaca ggatcaaggt ggtggaggga ggccccctgc cgttcgcatt cgacatcctg
      361 gccacccact ttatgtacgg gagcaaggtg ttcatcaagt accccgccga cctccccgat
      421 tattttaagc agtccttccc tgagggcttc acatgggaga gagtcatggt gttcgaagac
      481 gggggcgtgc tgaccgccac ccaggacacc agcctccagg acggcgagct catctacaac
      541 gtcaaggtca gaggggtgaa cttcccagcc aacggccccg tgatgcagaa gaaaacactg
      601 ggctgggagc ccagcaccga gaccatgtac cccgctgacg gcggcctgga aggcagatgc
      661 gacaaggccc tgaagctcgt gggcgggggc cacctgcacg tcaacttcaa gaccacatac
      721 aagtccaaga aacccgtgaa gatgcccggc gtccactacg tggaccgcag actggaaaga
      781 atcaaggagg ccgacaacga gacctacgtc gagcagtacg agcacgctgt ggccagatac
      841 tccaacctgg gcggaggcat ggacgagctg tacaagtaat gaattcgaag cttgatcc
//
