LOCUS       Exported                3702 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Gateway Donor vector containing STC2, part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    pDONR223_STC2_p.R5W
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      268..295
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      387..473
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1581..1680)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1697..1716)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1698..1716)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1721..1737)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1721..1737)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2168..2959
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3052..3640
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3541..3560
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atgtgtgccg agtggctggg ccagttcatg accctggctt tggtgttggc
      721 cacctttgac ccggcgcggg ggaccgacgc caccaaccca cccgagggtc cccaagacag
      781 gagctcccag cagaaaggcc gcctgtccct gcagaataca gcggagatcc agcactgttt
      841 ggtcaacgct ggcgatgtgg ggtgtggcgt gtttgaatgt ttcgagaaca actcttgtga
      901 gattcggggc ttacatggga tttgcatgac ttttctgcac aacgctggaa aatttgatgc
      961 ccagggcaag tcattcatca aagacgcctt gaaatgtaag gcccacgctc tgcggcacag
     1021 gttcggctgc ataagccgga agtgcccggc catcagggaa atggtgtccc agttgcagcg
     1081 ggaatgctac ctcaagcacg acctgtgcgc ggctgcccag gagaacaccc gggtgatagt
     1141 ggagatgatc catttcaagg acttgctgct gcacgaaccc tacgtggacc tcgtgaactt
     1201 gctgctgacc tgtggggagg aggtgaagga ggccatcacc cacagcgtgc aggttcagtg
     1261 tgagcagaac tggggaagcc tgtgctccat cttgagcttc tgcacctcgg ccatccagaa
     1321 gcctcccacg gcgccccccg agcgccagcc ccaggtggac agaaccaagc tctccagggc
     1381 ccaccacggg gaagcaggac atcacctccc agagcccagc agtagggaga ctggccgagg
     1441 tgccaagggt gagcgaggta gcaagagcca cccaaacgcc catgcccgag gcagagtcgg
     1501 gggccttggg gctcagggac cttccggaag cagcgagtgg gaagacgaac agtctgagta
     1561 ttctgatatc cggaggtgat tgccaacttt cttgtacaaa gttggcatta taagaaagca
     1621 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg
     1681 ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca
     1741 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
     1801 atgcctcctc tagaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc
     1861 cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc
     1921 ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac
     1981 cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt
     2041 tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg
     2101 tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt
     2161 aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg
     2221 cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt
     2281 ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct
     2341 tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg
     2401 agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc
     2461 gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct
     2521 tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc
     2581 aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc
     2641 tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga
     2701 ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt
     2761 aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc
     2821 ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg
     2881 cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac
     2941 caaggtagtc ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta
     3001 cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     3061 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     3121 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     3181 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     3241 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     3301 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     3361 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     3421 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     3481 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     3541 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     3601 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     3661 tttacggttc ctggcctttt gctggccttt tgctcacatg tt
//
