LOCUS Exported 3801 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Gateway Donor vector containing DARC, part of the Target Accelerator Plasmid Collection.. ACCESSION . VERSION . KEYWORDS pDONR223_DARC_p.T211S SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3801) AUTHORS Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS TITLE High-throughput Phenotyping of Lung Cancer Somatic Mutations. JOURNAL Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28. PUBMED 27478040 REFERENCE 2 (bases 1 to 3801) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3801 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1680..1779) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1796..1815) /label=T7 /note="T7 promoter, forward primer" promoter complement(1797..1815) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1820..1836) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1820..1836) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" CDS 2267..3058 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 3151..3739 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3640..3659 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agttggcatg gggaactgtc tgcacagggc ggagctctcc ccctcaactg agaactcaag 721 tcagctggac ttcgaagatg tatggaattc ttcctatggt gtgaatgatt ccttcccaga 781 tggagactat ggtgccaacc tggaagcagc tgccccctgc cactcctgta acctgctgga 841 tgactctgca ctgcccttct tcatcctcac cagtgtcctg ggtatcctag ctagcagcac 901 tgtcctcttc atgcttttca gacctctctt ccgctggcag ctctgccctg gctggcctgt 961 cctggcacag ctggctgtgg gcagtgccct cttcagcatt gtggtgcccg tcttggcccc 1021 agggctaggt agcactcgca gctctgccct gtgtagcctg ggctactgtg tctggtatgg 1081 ctcagccttt gcccaggctt tgctgctagg gtgccatgcc tccctgggcc acagactggg 1141 tgcaggccag gtcccaggcc tcaccctggg gctcactgtg ggaatttggg gagtggctgc 1201 cctactgaca ctgcctgtca ccctggccag tggtgcttct ggtggactct gcaccctgat 1261 atacagcacg gagctgaagg ctttgcaggc cacacactct gtagcctgtc ttgccatctt 1321 tgtcttgttg ccattgggtt tgtttggagc caaggggctg aagaaggcat tgggtatggg 1381 gccaggcccc tggatgaaca tcctgtgggc ctggtttatt ttctggtggc ctcatggggt 1441 ggttctagga ctggatttcc tggtgaggtc caagctgttg ctgttgtcaa catgtctggc 1501 ccagcaggct ctggacctgc tgctgaacct ggcagaagcc ctggcaattt tgcactgtgt 1561 ggctacgccc ctgctcctcg ccctattctg ccaccaggcc acccgcaccc tcttgccctc 1621 tctgcccctc cctgaaggat ggttttctca tctggacacc cttggaagca aatcctgatt 1681 gccaactttc ttgtacaaag ttgtcattat aagaaagcat tgcttatcaa tttgttgcaa 1741 cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg atatccccta 1801 tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa 1861 tctctgatgt tacattgcac aagataaaaa tatatcatca tgcctcctct agaccagcca 1921 ggacagaaat gcctcgactt cgctgctacc caaggttgcc gggtgacgca caccgtggaa 1981 acggatgaag gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa 2041 gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg 2101 cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg 2161 ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa 2221 cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacattatga gggaagcggt 2281 gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga 2341 accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca 2401 cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc 2461 tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc 2521 tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg 2581 cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc 2641 cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt 2701 ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc 2761 gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa 2821 tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa 2881 ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact 2941 tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca 3001 gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataacc 3061 ctcgagccac ccatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc 3121 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg 3181 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct 3241 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct 3301 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 3361 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 3421 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 3481 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 3541 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 3601 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 3661 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 3721 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 3781 ctggcctttt gctcacatgt t //