LOCUS       Exported                 978 bp ds-DNA     linear   SYN 24-MAY-2021
DEFINITION  Lentiviral vector for doxycycline-inducible expression of 
            constitutively active c-Src in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    Tet-CA-Src-GFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Kim NG, Gumbiner BM
  TITLE     Adhesion to fibronectin regulates Hippo signaling via the 
            FAK-Src-PI3K pathway.
  JOURNAL   J Cell Biol. 2015 Aug 3;210(3):503-15. doi: 10.1083/jcb.201501025. 
            Epub 2015 Jul 27.
  PUBMED    26216901
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 24, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    8..32
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             32..73
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
ORIGIN
        1 gatatcaaca agtttgtaca aaaaagttgg catggggagc agcaagagca agcccaagga
       61 ccccagccag cgccggcgca gcctggagcc acccgacagc acccaccacg ggggattccc
      121 agcctcgcag acccccaaca agacagcagc ccccgacacg caccgcaccc ccagccgctc
      181 ctttgggacc gtggccaccg agcccaagct cttcgggggc ttcaacactt ctgacaccgt
      241 cacgtcgccg cagcgtgccg gggcactggc tggcggcgtc accactttcg tggctctcta
      301 tgactacgag tcccggactg aaacggactt gtccttcaag aaaggagaac gcctgcagat
      361 tgtcaacaac acggaaggtg actggtggct ggctcattcc ctcactacag gacagacggg
      421 ctacatcccc agtaactatg tcgcgccctc agactccatc caggctgaag agtggtactt
      481 tgggaagatc actcgtcggg agtccgagcg gctgctgctc aaccccgaaa acccccgggg
      541 aaccttcttg gtccgggaga gcgagacgac aaaaggtgcc tattgcctct ccgtttctga
      601 ctttgacaac gccaaggggc tcaatgtgaa gcactacaag atccgcaagc tggacagcgg
      661 cggcttctac atcacctcac gcacacagtt cagcagcctg cagcagctgg tggcctacta
      721 ctccaaacat gctgatggct tgtgccaccg cctgaccaac gtctgcccca cgtccaagcc
      781 ccagacccag ggactcgcca aggacgcgtg ggaaatcccc cgggagtcgc tgcggctgga
      841 ggtgaagctg gggcagggct gctttggaga ggtctggatg gggacctgga acggcaccac
      901 cagagtggcc ataaagactc tgaagcccgg caccatgtcc ccggaggcct tcctgcagga
      961 agcccaagtg atgaagaa
//
