LOCUS       Exported                 921 bp ds-DNA     linear   SYN 17-JUN-2022
DEFINITION  recombinant AAV vector packaging self-complementary GFP under the 
            CAG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pscAAV-CAG-GFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Pekrun K, De Alencastro G, Luo QJ, Liu J, Kim Y, Nygaard S, Galivo 
            F, Zhang F, Song R, Tiffany MR, Xu J, Hebrok M, Grompe M, Kay MA
  TITLE     Using a barcoded AAV capsid library to select for clinically 
            relevant gene therapy vectors.
  JOURNAL   JCI Insight. 2019 Nov 14;4(22). pii: 131610. doi: 
            10.1172/jci.insight.131610.
  PUBMED    31723052
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 17, 2022 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      9..18
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             15..734
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             15..731
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(60..81)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(321..340)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     668..689
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     778..797
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 caccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
       61 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
      121 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
      181 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
      241 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
      301 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
      361 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
      421 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
      481 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
      541 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
      601 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
      661 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
      721 agctgtacaa gtaaagcggc cgctaggcct cacctgcgat ctcgatgctt tatttgtgaa
      781 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
      841 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaact
      901 agtccactcc ctctctgcgc g
//
