LOCUS Exported 4005 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Gateway Donor vector containing PTEN, part of the Target Accelerator Plasmid Collection.. ACCESSION . VERSION . KEYWORDS pDONR223_PTEN_p.H123Y SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4005) AUTHORS Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS TITLE High-throughput Phenotyping of Lung Cancer Somatic Mutations. JOURNAL Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28. PUBMED 27478040 REFERENCE 2 (bases 1 to 4005) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4005 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1884..1983) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(2000..2019) /label=T7 /note="T7 promoter, forward primer" promoter complement(2001..2019) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2024..2040) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2024..2040) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" CDS 2471..3262 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 3355..3943 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3844..3863 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agttggcacc atgacagcca tcatcaaaga gatcgttagc agaaacaaaa ggagatatca 721 agaggatgga ttcgacttag acttgaccta tatttatcca aacattattg ctatgggatt 781 tcctgcagaa agacttgaag gcgtatacag gaacaatatt gatgatgtag taaggttttt 841 ggattcaaag cataaaaacc attacaagat atacaatctt tgtgctgaaa gacattatga 901 caccgccaaa tttaattgca gagttgcaca atatcctttt gaagaccata acccaccaca 961 gctagaactt atcaaaccct tttgtgaaga tcttgaccaa tggctaagtg aagatgacaa 1021 tcatgttgca gcaatttact gtaaagctgg aaagggacga actggtgtaa tgatatgtgc 1081 atatttatta catcggggca aatttttaaa ggcacaagag gccctagatt tctatgggga 1141 agtaaggacc agagacaaaa agggagtaac tattcccagt cagaggcgct atgtgtatta 1201 ttatagctac ctgttaaaga atcatctgga ttatagacca gtggcactgt tgtttcacaa 1261 gatgatgttt gaaactattc caatgttcag tggcggaact tgcaatcctc agtttgtggt 1321 ctgccagcta aaggtgaaga tatattcctc caattcagga cccacacgac gggaagacaa 1381 gttcatgtac tttgagttcc ctcagccgtt acctgtgtgt ggtgatatca aagtagagtt 1441 cttccacaaa cagaacaaga tgctaaaaaa ggacaaaatg tttcactttt gggtaaatac 1501 attcttcata ccaggaccag aggaaacctc agaaaaagta gaaaatggaa gtctatgtga 1561 tcaagaaatc gatagcattt gcagtataga gcgtgcagat aatgacaagg aatatctagt 1621 acttacttta acaaaaaatg atcttgacaa agcaaataaa gacaaagcca accgatactt 1681 ttctccaaat tttaaggtga agctgtactt cacaaaaaca gtagaggagc cgtcaaatcc 1741 agaggctagc agttcaactt ctgtaacacc agatgttagt gacaatgaac ctgatcatta 1801 tagatattct gacaccactg actctgatcc agagaatgaa ccttttgatg aagatcagca 1861 tacacaaatt acaaaagtct gagacccagc tttcttgtac aaagttggca ttataagaaa 1921 gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat 1981 ttgccatcca gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg 2041 gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc 2101 atcatgcctc ctctagacca gccaggacag aaatgcctcg acttcgctgc tacccaaggt 2161 tgccgggtga cgcacaccgt ggaaacggat gaaggcacga acccagtgga cataagcctg 2221 ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt 2281 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt 2341 ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg 2401 atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa 2461 gttaaacatt atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt 2521 tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc 2581 agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag 2641 gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc 2701 tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat 2761 tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat 2821 tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa 2881 agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt 2941 tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc 3001 cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc 3061 agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc 3121 ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga 3181 tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat 3241 caccaaggta gtcggcaaat aaccctcgag ccacccatga ccaaaatccc ttaacgtgag 3301 ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga 3361 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt 3421 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag 3481 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa 3541 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag 3601 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca 3661 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac 3721 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa 3781 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc 3841 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg 3901 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc 3961 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtt //